From animal models to patients: the role of placental microRNAs, miR-210, miR-126, and miR-148a/152 in preeclampsia

Frazier, Sonya; McBride, Martin W.; Mulvana, Helen; Graham, Delyth

doi:10.1042/cs20200023

Cited by 37 publications

(30 citation statements)

References 267 publications

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“…MiR-126 influences the VEGF pathway through inhibition of Spred1 and lower levels of placental miR-126 in PE were associated with decreased VEGF placental expression [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

“…RNA enriched for small RNAs (including miRNAs) was isolated using the mirVana Paris Kit (Thermo Fisher). Reverse transcription and pre-amplification were performed using TaqMan miRNA primers (Thermo Fisher) and multiplex qPCR was done in a CFX96 thermal cycler (Bio-Rad) [ 25 ]. Raw C q values were calculated in Bio-Rad CFX manager software v.3.1 using automatic baseline and threshold settings.…”

Section: Methodsmentioning

confidence: 99%

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MicroRNAs targeting VEGF are related to vascular dysfunction in preeclampsia

et al. 2021

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In preeclampsia (PE) pre-existent maternal endothelial dysfunction leads to impaired placentation and vascular maladaptation. The vascular endothelial growth factor (VEGF) pathway is essential in the placentation process and VEGF expression is regulated through post-transcriptional modification by microRNAs. We investigated the expression of VEGF related circulating miR-16, miR-29b, miR-126, miR-155 and miR-200c in PE versus healthy pregnancies (HP), and their relation with vascular function, oxidative stress and systemic inflammation.In this case-control study, 24 women with early PE (in vivo vascular function (flow mediated dilation (FMD), modified FMD (mFMD), carotid-femoral pulse wave velocity (CF-PWV), augmentation index (AIx75) and reactive hyperaemia index (RHI)). FMD, CF-PWV, AIx75 and RHI were all significantly impaired in PE (p<0.05). PE patients had reduced levels of miR-16 (5.53±0.36vs5.84±0.61) and increased levels of miR-200c (1.34±0.57vs0.97±0.68) (p<0.05). Independent of age and parity, miR-16 was related to impaired FMD (ß 2.771, 95% C.I. 0.023-5.519, p=0.048) and mFMD (ß3.401, 95% C.I. 0.201-6.602, p=0.038). Likewise, miR-200c was independently associated with CF-PWV (ß0.513, 95% C.I. 0.034-0.992, p=0.036). In conclusion, circulating levels of miR-16 were lower in PE, which correlated with impaired endothelial function. Circulating miR-200c was increased in PE and correlated with higher arterial stiffness. These findings suggest a post-transcriptional dysregulation of the VEGF pathway in PE and identify miR-16 and miR-200c as possible diagnostic biomarkers for PE.

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“…MiR-126 influences the VEGF pathway through inhibition of Spred1 and lower levels of placental miR-126 in PE were associated with decreased VEGF placental expression [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

MicroRNAs targeting VEGF are related to vascular dysfunction in preeclampsia

et al. 2021

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“…In recent years, studies on early‐onset PE have gradually deepened into the molecular mechanism, and the role of miRNA in PE cannot be ignored 18, 25 . However, the pathway of action of miRNA is still unclear, so we studied the role of miR‐126 and VCAM‐1 in EOPE placental tissues and trophoblast cells, providing the possibility to elucidate the pathogenesis and treatment of EOPE.…”

Section: Discussionmentioning

confidence: 99%

“…Some studies have indicated that miR‐126 can regulate the expression of VCAM‐1 in lung cancer, gestational diabetes mellitus, atherosclerosis, and other diseases 15–17 . Previous studies have found that miR‐126 and VCAM‐1 are respectively involved in the occurrence and development of PE, 18–20 however, there is no report on the correlation between miR‐126 and VCAM‐1 in PE. In this study, we detected the expression of miR‐126 and VCAM‐1 in placental tissue and trophoblast to explore the role of miR‐126 and VCAM‐1 in the pathogenesis of early‐onset PE.…”

Section: Introductionmentioning

confidence: 99%

Expression and significance of microRNA‐126 and VCAM‐1 in placental tissues of women with early‐onset preeclampsia

Liu

Cui

et al. 2021

J of Obstet and Gynaecol

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Objective To investigate the expression of microRNA‐126 (miR‐126) and vascular endothelial cell adhesion molecule‐1 (VCAM‐1) in the placental tissues of women with early‐onset preeclampsia (EOPE) and their effects on trophoblast invasion. Materials and Methods The placental tissues of 30 pregnant women with EOPE who delivered in the Third Affiliated Hospital of Zhengzhou University from November 2019 to May 2020 were selected as the preeclampsia (PE) group, and the placental tissues of 30 healthy pregnant women with normal prenatal examination were selected as the normal group. Immunohistochemistry was used to localize VCAM‐1 in placental tissues，the expression of miR‐126 and VCAM‐1 in placenta tissues of two groups and HTR‐8/SVneo cells transfected with miR‐126 were detected by real‐time polymerase chain reaction (RT‐PCR) and Western blot, and the correlation between them was analyzed. The invasion ability of cells transfected with miR‐126 was observed by Transwell invasion test. Results Compared with the normal group, the expression of miR‐126 was higher and VCAM‐1 was lower in the placental tissues of the PE group, and the difference were statistically significant (p < 0.01). Moreover, VCAM‐1 was negatively correlated with the expression of miR‐126 (r = −0.391, p < 0.05). In vitro experiment, the expression level of VCAM‐1 in miR‐126 mimics transfection group was decreased, and the expression level of VCAM‐1 in miR‐126 inhibitor transfection group was increased; the invasion ability of HTR‐8/SVneo cells transfected with miR‐126 mimics was decreased, and the invasion ability of HTR‐8/SVneo cells transfected with miR‐126 inhibitor was enhanced. Conclusion There was a negative correlation between the expression of miR‐126 and VCAM‐1 in EOPE.MiR‐126 and VCAM‐1 may participate in the occurrence and development of EOPE by affecting the invasion ability of trophoblast cells.

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“…[35,36] Another three preeclampsia rodent model studies show elevated miR-210 in part driven by HIF-1α and NF-κBp50, induced by hypoxia and/or immune-mediated processes may contribute in preeclampsia by inhibiting anti-inflammatory Th2-cytokines. [37] miRNA125b as Biomarker Third Trimester of Pregnancy Hypertensive Disorder miR-125b in pregnant women can be detected from several different sites, the plasma from the mother, where it is often called the maternal circulating miR-125b and the placental cells, where it is called as placental miR-125b. [38][39][40] Circulating miR-125b supposedly comes from the placenta itself, as one in vivo study suggests, making the maternal circulating miR-125b and placental miR-125 closely related to one another.…”

Section: Methodsmentioning

confidence: 99%

MicroRNA-125b as a Preeclampsia Biomarker

2021

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Pregnancy hypertensive disorder (PHD) is a broad term referring to conditions related to disorders of high blood pressure during pregnancy. Preeclampsia is one of 4 types of PHD characterized by an increase in systolic blood pressure ≤ 140 mmHg with a diastolic pressure ≤ 90 mmHg accompanied by proteinuria of 1+ or more. Complications of PHD include peripartum cardiomyopathy, maternal mortality, pulmonary edema, cerebrovascular accident and renal failure. As consequence, early detection of PHD is crucial in preventing complications. “Preeclampsia”, “microRNA-125b” and “Biomarker” were the keywords applied to scientific online databases, such as ScienceDirect, PubMed, and Researchgate. A total of 46 journals were screened, reviewed and utilized with utmost precision to construct this literature review. Looking at the dangers of preeclampsia on infants and mothers, miRNA-125b has potential to be used as a biomarker of preeclampsia that can occur in the third trimester. The increase in the concentration of miRNA-125b in the first trimester causes inhibition of trophoblast invasion, which is followed by inhibition of KCNA1, GPC1, and SGPL1. All of these proteins play a role in the invasion trophoblast which is also exacerbated by the increase in the concentration of mir-125b. All in all, these symptoms and the increase of mir-125b that appear in the first trimester yield as a promising result as biomarkers of preeclampsia in the third trimester. Keywords: preeclampsia, microRNA-125b, biomarker, SGPL1, maternal, infants.

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From animal models to patients: the role of placental microRNAs, miR-210, miR-126, and miR-148a/152 in preeclampsia

Cited by 37 publications

References 267 publications

MicroRNAs targeting VEGF are related to vascular dysfunction in preeclampsia

MicroRNAs targeting VEGF are related to vascular dysfunction in preeclampsia

Expression and significance of microRNA‐126 and VCAM‐1 in placental tissues of women with early‐onset preeclampsia

MicroRNA-125b as a Preeclampsia Biomarker

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