Frequency of sister chromatid exchanges in the lymphocytes of patients with atopic dermatitis

Abstract

The combination of genetic susceptibility and environmental factors can induce allergic sensitization and subsequent local inflammation, resulting in atopic dermatitis (AD). Sister chromatid exchange (SCE) is a sensitive method that may reflect an instability in DNA or a deficiency in DNA repair. The aim of the present study was to investigate whether patients with AD have defects in DNA repair and whether SCE frequency can be used as a genetic marker in the pathogenesis of AD. Between September 2004 and July 2005, SCE was analyzed in the peripheral blood lymphocyte chromosomes of 32 patients with AD and 28 control subjects at the Dermatology Unit of Erzurum State Hospital. This study found that the SCE frequency was significantly increased in patients with AD (P < 0.00001). The prevalence of SCE was not correlated with patient age, sex, disease duration or AD disease severity. Our results indicate that increased chromosome instability may play an important role in the etiology of AD.

Sister chromatid exchange analysis in patients with psoriasis

“…However, these observations were based on limited number of patients; the results may be explained by elevated rate of DNA damage and defect in DNA repair in psoriasis. Similarly, we demonstrated increased SCE frequencies in patients with atopic dermatitis (42).…”

Genotoxicity assessment in iron deficiency anemia patients using sister chromatid exchanges and chromosomal aberrations assays

“…SCEs and CAs occur spontaneously in all cells and increase by certain treatments or associated with diseases [29][30][31].…”

The algorithm for identification of the etiology of atopic dermatitis torpid forms

“…Вполне вероятно, что мы недооцениваем роль хромосомной нестабильности и дефектов репара-ции ДНК в этиологии АД. Так A. Karaman и C. Aliağaoğlu [21] показали, что сестринские хрома-тидные обмены (SCE) значительно повышены у па-циентов с АтД независимо от пола, возраста, про-должительности и тяжести заболевания. Расшире-ние технических возможностей молекулярно-био-логических исследований в настоящее время суще-ственно опережает их практическое использование в медицинской практике.…”