Frequency of FCGR3B Alleles in Thai Blood Donors

Abstract

BackgroundHuman neutrophil antigens (HNAs) are involved in autoimmune and alloimmune neutropenia and transfusion-related acute lung injury. The HNA-1 system is important in immunogenetics, and allele frequencies have been described in different populations. This study investigated the frequency of FCGR3B alleles encoding HNA-1a, HNA-1b, and HNA-1c among Thai blood donors and compared these frequencies with those previously reported for other populations.MethodsEight hundred DNA samples obtained from unrelated healthy blood donors at the National Blood Centre, Thai Red Cross Society, Bangkok, and the Blood Bank, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand, were included. Samples were simultaneously typed for each FCGR3B allele using an in-house polymerase chain reaction with sequence-specific primer (PCR-SSP) technique.ResultsThe frequencies of FCGR3B*1, FCGR3B*2, and FCGR3B*3 alleles in central Thai blood donors were 0.548, 0.452, and 0.004, respectively; only FCGR3B*1 and FCGR3B*2 alleles were found in northern Thai blood donors (0.68 and 0.32, respectively). Compared with other Asian populations, central Thais had higher frequencies of the FCGR3B*2 allele (P<0.001), while the frequencies of the FCGR3B*1 and FCGR3B*2 alleles in northern Thais were similar to those previously reported in Taiwanese and Japanese populations. In contrast, the frequencies of the FCGR3B*1 and FCGR3B*2 alleles in the northern Thai population were statistically different from those observed in central Thai, Korean, German, and Turkish populations.ConclusionsFCGR3B allele frequencies were significantly different between central and northern Thai blood donors. Our in-house PCR-SSP method is a simple, cost-effective, and convenient method for FCGR3B allele detection.

Assessment of HNA alloimmunisation risk in Northeastern Thais, Burmese and Karen

“…In ethnic Thai populations, the predicted risk of HNA-1 incompatibility observed in Northeastern Thais was different from those reported in Central Thais. The reason for this discrepancy may simply be reflected by sampling and sample size of the cohorts (Kaset et al, 2013;Changsri et al, 2014). Compared with previous studies of other populations, the predicted risk of HNA-1 incompatibility and alloimmunisation in our study was similar to Asian populations (except Asian Indian and Central Thais) but was significantly different (P < 0·05) from Caucasian and African populations.…”

“…Although there have been studies of HNA allele frequencies in some ethnic Thai populations (Kaset et al, 2013;Changsri et al, 2014;Kongmaroeng & Kumkaen, 2015), none of these studies involved the population from northeastern Thailand as well as the Burmese and Karen populations.…”

A study of human neutrophil antigen genotype frequencies in Hong Kong

“…The current cohort examined samples of 300 subjects randomly selected from healthy blood donors in Red Cross Blood Transfusion Service, and it provides a good representation of the local Chinese population and is found comparable with other studies (Fujiwara et al, ; Steffensen et al, ; Kissel et al, ; Flesch et al, ; Hauck et al, ; Xia et al, ; Matsuhashi et al, ; Nielsen et al, ; Cardoso et al, ; Kaset et al, ; Changsri et al, ; He et al, ). We also added additional information on the HNA‐2 allele of the Chinese population.…”

“…Han (Xia et al, 2011) Japanese (Matsuhashi et al, 2012) Central Thai (Kaset et al, 2013) Thai (Changsri et al, 2014) German (Hauck et al, 2011) British (Cardoso et al, 2013) Australian (Wu et al, 2016) USA (Li et al, 2015) Antigen…”

FCGR3B gene frequencies among ethnic Thai blood donors from a regional hospital in Eastern Thailand

“…In this study, the result shows that the frequency of FCGR3B in the Eastern Thais is similar to the previous published data regarding Northern Thais and other Asian populations (Chinese Han, Taiwanese, and Japanese), in whom FCGR3B*01 was found more frequently than FCGR3B*02 . Comparison of the allele frequencies between ethnic Thai populations shows a similarity in allele frequencies between Eastern and Northern Thais, whereas these frequencies are significantly different from Central Thais, where FCGR3B*02 is the high‐frequency antigen . These results suggest the genetic difference of the Central Thais, resulting in migration and marriage between Thais and different ethnic populations, particularly around the area of Bangkok.…”