Assessment of <scp>HNA</scp> alloimmunisation risk in Northeastern Thais, Burmese and Karen

Simtong, Piyapong; Puapairoj, Chintana; Leelayuwat, Chanvit; Romphruk, Amornrat

doi:10.1111/tme.12431

Cited by 9 publications

(23 citation statements)

References 45 publications

(57 reference statements)

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“…The genotype frequencies of heterozygous HNA-1a/b between the Chinese and populations of German and British were similar, whereas the gene frequencies of HNA-1a/b between the Chinese and populations of Danish and Zambians were quite different. Similar findings have also been reported by Simtong et al (2017). As antibodies against HNA-1b have been associated with ANN (Tomicic et al, 2009), the lower HNA-1b gene frequency, consistent with reports in other Asian populations, may suggest that there may be a lower incidence rate of ANN in Asians compared to some Western countries.…”

Section: Comparing Hna Allele and Genotype Frequencies In Hong Kong Csupporting

confidence: 88%

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A study of human neutrophil antigen genotype frequencies in Hong Kong

Tam

Tang

et al. 2017

Transfusion Medicine

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This is the first study to define HNA genotype and allele frequencies using a validated modified in-house PCR-SSP method in the Hong Kong Chinese blood donor population. Our approach provides a cost-effective assay for conducting routine HNA typing and facilitates the incorporation of these assays into routine clinical service. Our results are comparable with those reported in the Guangzhou Chinese population, but the allele frequencies in our Hong Kong Chinese population are significantly different from the reported European frequencies, confirming that a geographical difference exists for HNA allele frequencies.

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Section: Comparing Hna Allele and Genotype Frequencies In Hong Kong Csupporting

confidence: 88%

“…Similar findings have also been reported by Simtong et al . (). As antibodies against HNA‐1b have been associated with ANN (Tomicic et al, ), the lower HNA‐1b gene frequency, consistent with reports in other Asian populations, may suggest that there may be a lower incidence rate of ANN in Asians compared to some Western countries.…”

Section: Discussionsupporting

confidence: 60%

A study of human neutrophil antigen genotype frequencies in Hong Kong

Tam

Tang

et al. 2017

Transfusion Medicine

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“…Although there has been studied on RBC phenotype frequencies in ethnic Thai populations (Fongsarun et al, ), there are no data from north‐east Thailand. Our previous studies on other aspects of molecular genetics, such as human leucocyte antigens (HLA), human neutrophil antigens (HNA) and killer immunoglobulin‐like receptors (KIRs), revealed significant differences in frequency of alleles/molecules in different regions of Thailand (Chaisri et al, ; Romphruk et al, ; Simtong et al, ). Here, we report for the first time the antigen and phenotype frequencies of blood group systems (Rh, M, Mi a and Kidd) by gel technology among ethnic Thai blood donors in the north‐east of Thailand.…”

Section: Discussionmentioning

confidence: 99%

Phenotype frequencies of Rh (C, c, E, e), M, Mi^a and Kidd blood group systems among ethnic Thai blood donors from the north‐east of Thailand

Romphruk

Butryojantho

Jirasakonpat

et al. 2019

Int J Immunogenetics

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We here report the first study of antigen and phenotype frequencies of Rh (C, c, E, e), M, Mia and Kidd antigens in north‐east Thai blood donors. Blood transfusion services aim to ensure availability of adequate and safe blood to minimize the development of transfusion reactions. For pre‐transfusion testing, the most important blood group systems are ABO and RhD. The transfusion of ABO‐compatible otherwise unknown phenotype blood may result in alloimmunization, especially in multi‐transfused patients. Extended red blood cell (RBC) phenotyping and selection of blood negative for specific antigens reduce post‐transfusion complications and allow for effective blood transfusion regimens to be achieved. A total of 13,567 regular repeated, voluntary Thai blood donors were included for red‐cell antigen typing of Rh (D, C, E, c, e). Samples from 12,768, 9,389 and 13,059 donors were typed for Kidd, M and Mia antigens, respectively. Amongst Rh antigens, e was the most common (96.80%) followed by C (95.50%), c (34.40%) and E (32.20%) with CCDee (60.00%) being the most common phenotype. For Kidd phenotypes, Jk(a+b+) was the most common (46.73%) and Jk(a−b−) was rare (0.07%). For the M and Mia antigen, M(+) was most frequently found (94.96%) and Mia(+) was found in 17.97% of individuals. Knowledge of red‐cell antigen phenotype frequencies in a population is helpful for creating a phenotype database of blood donors which can provide antigen‐negative compatible blood to patients with multiple alloantibodies. Moreover, provision of antigen‐matched blood can prevent alloimmunization in multi‐transfused patients.

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“…Until today, this fact impedes routine antibody screening for many laboratories. In addition, neutrophil donors expressing rare HNA antigen (such as HNA‐1c) are difficult to obtain, especially in certain regions with low allelic frequency (Asian compared to Caucasian population) . However, anti‐HNA‐1c alloantibodies play an important role on the mechanism of severe NAIN .…”

Section: Discussionmentioning

confidence: 99%

“…In addition, neutrophil donors expressing rare HNA antigen (such as HNA-1c) are difficult to obtain, especially in certain regions with low allelic frequency (Asian compared to Caucasian population). 22 However, anti-HNA-1c alloantibodies play an important role on the mechanism of severe NAIN. 23 Recent data from our laboratory indicated that anti-HNA-1c alloantibodies aroused more frequently than expected, but may often be covered by the presence of anti-HNA-1b alloantibodies in serum samples.…”

Section: Discussionmentioning

confidence: 99%

Improvement of monoclonal antibody–immobilized granulocyte antigen assay for the detection of anti‐HNA‐1 alloantibodies

et al. 2017

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BACKGROUND Currently, the gold standard for the identification of antibodies against human neutrophil antigens (HNAs) is the monoclonal antibody–immobilized granulocyte antigen (MAIGA) assay. However, the handling of this assay is laborious and therefore cumbersome for the rapid screening of neutrophil antibodies. STUDY DESIGN AND METHODS In this study, we simplified the performance of the conventional MAIGA procedure and approved it for the identification of anti‐HNA‐1 with HNA‐1–typed neutrophils and stable transfected (HEK293) cell line expressing HNA‐1a, ‐1b, and ‐1c. RESULTS In contrast to the conventional MAIGA, all working steps including the incubation of antibodies with cells, washings, cell lysis, and subsequent antibody detection could be performed on microtiter plates. This modification accelerates the work schedule of MAIGA and reduces pipetting errors. Comparison between both assay performances using neutrophils as target showed concordant results. Subsequently, stable mammalian cell lines were tested. In comparison to neutrophils, cell lines were stable for a longer period of time (>4 weeks) and results obtained with these cell lines showed better interassay precision. Analysis of different FcγRIIIb capture monoclonal antibodies (MoAbs) for the MAIGA assay showed that MoAb LNK16 is superior for the detection of anti‐HNA‐1a, ‐1b, and ‐1c, whereas MoAb 3G8 showed false‐negative results, caused by competitive inhibition of anti‐HNA‐1c alloantibodies. CONCLUSION The modification of MAIGA and the use of transfected HEK293 cells improve the detection of anti‐HNA‐1 alloantibodies that may allow screening analysis in large cohort of samples.

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Assessment of HNA alloimmunisation risk in Northeastern Thais, Burmese and Karen

Abstract: This is the first study that reported the allele frequencies of HNA-1, -3, -4, and -5 in a large sample of healthy unrelated individuals from ethnic Thais, Burmese and Karen. Our results indicated the high possible risk of HNA-1, -3 and -5 alloimmunisation in these populations.

Cited by 9 publications

References 45 publications

A study of human neutrophil antigen genotype frequencies in Hong Kong

A study of human neutrophil antigen genotype frequencies in Hong Kong

Phenotype frequencies of Rh (C, c, E, e), M, Mi^a and Kidd blood group systems among ethnic Thai blood donors from the north‐east of Thailand

Improvement of monoclonal antibody–immobilized granulocyte antigen assay for the detection of anti‐HNA‐1 alloantibodies

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Assessment of HNA alloimmunisation risk in Northeastern Thais, Burmese and Karen

Abstract: This is the first study that reported the allele frequencies of HNA-1, -3, -4, and -5 in a large sample of healthy unrelated individuals from ethnic Thais, Burmese and Karen. Our results indicated the high possible risk of HNA-1, -3 and -5 alloimmunisation in these populations.

Cited by 9 publications

References 45 publications

A study of human neutrophil antigen genotype frequencies in Hong Kong

A study of human neutrophil antigen genotype frequencies in Hong Kong

Phenotype frequencies of Rh (C, c, E, e), M, Mia and Kidd blood group systems among ethnic Thai blood donors from the north‐east of Thailand

Improvement of monoclonal antibody–immobilized granulocyte antigen assay for the detection of anti‐HNA‐1 alloantibodies

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Phenotype frequencies of Rh (C, c, E, e), M, Mi^a and Kidd blood group systems among ethnic Thai blood donors from the north‐east of Thailand