Freezing tolerance and its relationship with soluble carbohydrates, proline and water content in 12 grapevine cultivars

Ahmad, Ejaz; Karimi, Rouhollah; Mahdei, Karim Naderi

doi:10.1007/s11738-015-2021-6

Cited by 61 publications

(26 citation statements)

References 27 publications

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“…LT50 is used as a standard index to assess cold hardiness in plants [31]. According to our results, the REL at different temperatures showed an acceptable 'S' curve; therefore, LT50 could be calculated using the logistic sigmoid function method.…”

Section: Evaluation Of Cold Resistancementioning

confidence: 77%

Genome-wide DNA polymorphisms in four Actinidia arguta genotypes based on whole-genome re-sequencing

Lin¹,

Fang²,

Hu³

et al. 2020

PLoS ONE

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Among the genus Actinidia, Actinidia arguta possesses the strongest cold resistance and produces fresh fruit with an intense flavor. To investigate genomic variation that may contribute to variation in phenotypic traits, we performed whole-genome re-sequencing of four A. arguta genotypes originating from different regions in China and identified the polymorphisms using InDel markers. In total,4,710,650,4,787,750,4,646,026,and 4,590,616 SNPs and 1,481,002,1,534,198,1,471,304,and 1,425,393 InDels were detected in the 'Ruby-3', 'Yongfeng male', 'Kuilv male', and 'Hongbei male' genomes, respectively, compared with the reference genome sequence of cv 'Hongyang'. A subset of 120 InDels were selected for re-sequencing validation. Additionally, genes related to non-synonymous SNPs and InDels in coding domain sequences were screened for functional analysis. The analysis of GO and KEGG showed that genes involved in cellular responses to water deprivation, sucrose transport, decreased oxygen levels and plant hormone signal transduction were significantly enriched in A. arguta. The results of this study provide insight into the genomic variation of kiwifruit and can inform future research on molecular breeding to improve cold resistance in kiwifruit. OPEN ACCESS Citation: Lin M, Fang J, Hu C, Qi X, Sun S, Chen J, et al. (2020) Genome-wide DNA polymorphisms in four Actinidia arguta genotypes based on wholegenome re-sequencing. PLoS ONE 15(4): e0219884. https://doi.org/10.regulated by CBF transcription factors. In a previous study, CBF was cloned from the fruit of kiwifruit plants, and the expression of CBF was found to be increased when fruit was stored at low temperatures [6]. Proteomics analysis showed differences in proteins involved in photosynthesis, sugar metabolism, gene regulation, signal transduction, and stress resistance under lowtemperature stress in A. arguta leaves [7]. Despite the extensive knowledge regarding the coldrelated changes in A. arguta, the genetic components underlying these differences remain poorly understood, and associated genomic information for this species is lacking.The basic chromosome number of kiwifruit (x = 29) is high compared with those of other horticultural crops, and the genus presents extensive inter-taxal and intra-taxal variations in ploidy [1]. The sequence of the kiwifruit variety cv 'Hongyang', which was the first species in Ericales to be sequenced, represents a valuable resource not only for biological discovery and crop improvement but also for evolutionary and comparative genomic analysis. The sequence assembly covers~80% of the estimated genome size of 758 Mb [8], and its annotation revealed 39,040 predicted genes [9]. The genome annotation data of cv 'Hongyang' were updated in 2015, including 20 genes that were revised and 30 genes that were created (http://bdg.hfut.edu. cn/kir/index.html). Mining of microRNAs in the cv 'Hongyang' genome and transcriptome has led to the identification of 58 putative microRNAs in kiwifruit [10]. Li utilized this genome sequence to profi...

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Section: Evaluation Of Cold Resistancementioning

confidence: 77%

Genome-wide DNA polymorphisms in four Actinidia arguta genotypes based on whole-genome re-sequencing

Lin¹,

Fang²,

Hu³

et al. 2020

PLoS ONE

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show abstract

“…LT50 is used as a standard index to assess cold hardiness in plants(Ershadi et al 2015). According to our results, the REL at different temperatures showed an acceptable ‘S’ curve; therefore, LT50 could be calculated using the logistic sigmoid function method.…”

Section: Resultsmentioning

confidence: 99%

Genome-wide DNA polymorphisms in four Actinidia arguta genotypes based on whole-genome re-sequencing

Fang¹,

Lin

et al. 2019

Preprint

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AbstractAmong the genus Actinidia, Actinidia arguta possesses the strongest cold resistance and produces fresh fruit with an intense flavor. To investigate genomic variation that may contribute to variation in phenotypic traits, we performed whole-genome re-sequencing of four A. arguta genotypes originating from different regions in China and identified the polymorphisms using InDel markers. In total, 4,710,650, 4,787,750, 4,646,026, and 4,590,616 SNPs and 1,481,002, 1,534,198, 1,471,304, and 1,425,393 InDels were detected in the ‘Ruby-3’, ‘Yongfeng male’, ‘Kuilv male’, and ‘Hongbei male’ genomes, respectively, compared with the reference genome sequence of ‘Hongyang’. A subset of 120 InDels were selected for re-sequencing validation. Additionally, genes related to non-synonymous SNPs and InDels in coding domain sequences were screened for functional analysis. The analysis of GO and KEGG showed that genes involved in cellular responses to water deprivation, sucrose transport, decreased oxygen levels and plant hormone signal transduction were significantly enriched in A. arguta. The results of this study provide insight into the genomic variation of kiwifruit and can inform future research on molecular breeding to improve cold resistance in kiwifruit.

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“…The indicators of the water regime were determined by the gravimetric method and were expressed in % of the wet weight. The content of soluble sugars was determined using an anthrone reagent according to the method [15]. The content of soluble proteins was determined using a Unico 2800 spectrophotometer [16].…”

Section: Objects -4 Varieties Of Apple Treesmentioning

confidence: 99%

Adaptive resistance of apple trees in the unstable climate of southern Russia

et al. 2021

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The purpose of these studies is to assess the adaptive resistance of the apple tree to winter stresses in terms of physiological and biochemical parameters, to identify the most resistant varieties for cultivation in the Krasnodar region. Objects of research are apple varieties of various origins: Orfey, Idared, Ligol, Prikubanskoe. It was found that the cryoprotective function of water-soluble sugars was maximally manifested in the varieties Orfey and Ligol, their content increased by 2.56 and 2.7 times, respectively. Soluble proteins made the greatest contribution to the formation of a protective response in Orfey and Prikubanskoye varieties in January, their content increased 1.8 and 1.9 times, respectively, compared to December. The protective function of anthocyanins was maximally manifested in December in the Orfey variety, in the bark of which their content increased by 5.6 times. Physiological and biochemical adaptation of apple varieties to low negative temperatures of the winter period is achieved by increasing the content of water-soluble sugars, proteins in anthocyanins in the bark and buds. According to the obtained data, the apple varieties Orfey and Prikubanskoye showed themselves to be more adaptive in comparison with other studied varieties and are recommended for cultivation in the Krasnodar region.

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Freezing tolerance and its relationship with soluble carbohydrates, proline and water content in 12 grapevine cultivars

Cited by 61 publications

References 27 publications

Genome-wide DNA polymorphisms in four Actinidia arguta genotypes based on whole-genome re-sequencing

Genome-wide DNA polymorphisms in four Actinidia arguta genotypes based on whole-genome re-sequencing

Genome-wide DNA polymorphisms in four Actinidia arguta genotypes based on whole-genome re-sequencing

Adaptive resistance of apple trees in the unstable climate of southern Russia

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