Free Radicals Scavenging Capacity, Antidiabetic and Antihypertensive Activities of Flavonoid‐Rich Fractions from Leaves of Trichilia emetica and Opilia amentacea in an Animal Model of Type 2 Diabetes Mellitus

Abstract

Trichilia emetica and Opilia amentacea traditional Burkinabe medicinal plants were investigated to determine their therapeutic potential to inhibit key enzymes in carbohydrate metabolism, which has relevance to the management of type 2 diabetes. In vitro and in vivo antioxidant and antihypertensive potential and antilipidemia and antihyperglycemia activities in an animal model of type 2 diabetes mellitus have been studied. The antioxidant activity of the flavonoids from leaves of Trichilia emetica and Opilia amentacea has been evaluated using β-carotene-linoleic acid system, 1,1-diphenyl-2-picrylhydrazyl inhibitory activity, chelation of iron (II) ions, and lipid peroxidation which showed more pronounced antioxidant capacities of Trichilia emetica. Total cholesterol concentrations decreased in an animal model of type 2 diabetes mellitus under effects of flavonoid-rich fractions from leaves of Trichilia emetica and Opilia amentacea has been observed. Extract of flavonoid-rich fractions from Trichilia emetica shown maximum radical scavenging activity and possessed marked antiamylase activity which may be due to the presence of certain secondary metabolites. Suggested better antihyperglycemia, antilipidemia, and antihypertensive properties of flavonoid-rich fractions from Trichilia emetica compared to the extract of Opilia amentacea are demonstrating antidiabetic potential of Trichilia emetica as therapeutic targets for the management of type 2 diabetes.

In Vitro and In Vivo Antioxidant Properties of Taraxacum officinale in Nω-Nitro-l-Arginine Methyl Ester (L-NAME)-Induced Hypertensive Rats

“…This study corroborates the results presented by Yarnel and Abascal (2009) who showed that up to 6 g/kg, the T. officinale extract was not toxic in rabbits [29]. Plant extracts and pharmacological substances with LD 50 less than 5 mg/kg bw are classified in the range of highly toxic, those with LD 50 between 5 mg/kg and 5000 mg/kg bw are classified as moderately toxic; while those with LD 50 more than 5000 mg/kg bw are not toxic [30].…”

Phytochemical composition, and analgesic and antiinflammatory properties of essential oil of <i>Chamaemelum nobile</i> (Asteraceae L All) in rodents

“…xenobiotics. Pharmacological substances with LD 50 lower than 5 mg/kg body weight are considered highly toxic; those with LD 50 between 5 mg/kg and 5000 mg/kg body weight are classified as moderately toxic; while those with LD 50 greater than 5000 mg/kg body weight are described as being non-toxic [19].…”

Preventive effect of lemon seed flavonoids on carbon tetrachloride-induced liver injury in mice

“…4 At the same time, the research shows that the Trichilia emetica leaf is rich in avonoids, which has the in vivo effect of treating type 2 diabetes by target therapy. 5 It has also been shown that a polyoxyavone from citrus, nobiletin, shows anti-inammatory and anti angiogenic activities in zebrash in vivo. 6 Natural polyphenol avonoid also has anticancer effect, quercetin can inhibit the establishment of tumor in nude mice by subcutaneously inoculating HepG2 cells, and its anticancer effect can be achieved by regulating the expression of cyclin D1 in cancer cells.…”