Free radical scavenging activity of Lactobacillus fermentum in vitro and its antioxidative effect on growing-finishing pigs

Abstract

Aims: To evaluate the free radical‐scavenging capacity of Lactobacillus fermentum and its effects on antioxidant enzyme levels in finishing pigs. Methods and Results: The free radical‐scavenging activity of Lact. fermentum was analysed in vitro. The tested Lactobacillus showed a high scavenging ability against DPPH (1,1‐diphenyl‐2‐picrylhydrazyl), superoxide and hydroxyl radicals which was dose dependent. Subsequently, 108 crossbred pigs weighing 20·67 BW, were allotted to dietary treatments including a basal diet or the basal diet supplemented with either aureomycin or 10·2 × 107 Lact. fermentum CFU g−1 diet. Supplementation of Lact. fermentum increased total antioxidant capacity (P < 0·01) in serum from 50 kg pigs, while serum superoxide dismutase (P = 0·01) and glutathione peroxidase (P < 0·01) increased, and malondialdehyde levels decreased (P < 0·01) in 90 kg pigs. Hepatic catalase (P = 0·04), muscle superoxide dismutase (P < 0·01) and copper–zinc‐superoxide dismutase were enhanced (P = 0·01), whereas malondialdehyde levels were reduced (P = 0·05) by Lact. fermentum. Conclusions: The free radical‐scavenging capacity of Lact. fermentum was dose dependent and its supplementation improved the antioxidant status of pigs. Significance and Impact of the Study: Lactobacillus fermentum could be used to alleviate oxidative stress and increase pig performance and improve pork quality.

Probiotic and Antioxidative Properties ofL. LactisLL27 Isolated from Milk

“…Free radical scavenging is one of the mechanisms by which antioxidants inhibit lipid peroxidation (32,45). An in vitro ability of Lactobacillus and Bifidobacterium to quench free radicals has been reported in a few studies as well (29,57). These data, together with our findings, suggest that the DPPh radical scavenging activity is genus-, strain-and dosedependent.…”

Administration of <i>Lactobacillus fermentum</i> I5007 to young piglets improved their health and growth

“…We previously reported that L. fermentum had several properties of an excellent probiotic, including high adhesion ability to Caco-2 cells as well as competitiveness against Salmonella and E. coli . Moreover, our group has shown that this L. fermentum strain had the ability to scavenge free radicals in vitro (Wang et al, 2009). In the present experiment, the glutathione concentration, glutathione peroxidase activity and total antioxidant capacity in the plasma of piglets administered L. fermentum were all greater compared with the control group.…”

“…also found that oral administration of L. fermentum increased growth performance and improved immune function in weaned piglets. Moreover, our group has shown that this strain of L. fermentum had the ability to scavenge free radicals in vitro, enhanced the antioxidant status of piglets (Wang et al, 2009(Wang et al, , 2013, and affected the small intestinal proteomes of weaning piglets .…”

Antioxidative potential of folate producing probiotic Lactobacillus helveticus CD6

“…The ICFE of isolate showed hydroxyl radical scavenging activity equivalent with~8 μg of ascorbic acid in vitro. The % hydroxyl radical scavenging activity was found to be comparable with Lactobacillus fermentum (Wang et al 2009). In addition, the iron chelation states inhibition of hydroxyl radical formation via Fenton reaction.…”