Probiotic and Antioxidative Properties of<i>L. Lactis</i>LL27 Isolated from Milk

Özdoğan, Dilek Kaya; Akçelik, Nefise; Aslım, Belma; Suludere, Zekiye; Akçelik, Mustafa

doi:10.5504/bbeq.2011.0091

Cited by 7 publications

(3 citation statements)

References 56 publications

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“…The indiscriminate use of antibiotics as growth promoters in human and veterinary medicine leads to the production of drug-resistant strains among microorganisms (Robredo et al, 2000). Strong susceptibility had shown by NMCC-M16 towards all the selected antibiotics, while for ampicillin it showed intermediate resistance previous finding is correspondent with the susceptibility spectrum of Lactococcus lactis (Khemariya et al, 2013;Liasi et al, 2009;Ozdogan et al, 2012). In contrast NMCC-f13, NMCC-f17 and NMCC-M1 are sensitive to Chloramphenicol, Gentamycin, Kanamycin and Tetracycline, but mainly resistant to ampicillin and streptomycin.…”

Section: Listeria Monocytogenesmentioning

confidence: 92%

Tracing probiotic producing bacterial species from gut of buffalo (Bubalus bubalis), South-East-Asia

et al. 2024

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Due to extensive application of antibiotics as growth promoters in animal feed, antimicrobial resistance has been increased. To overcome this challenge, rumen microbiologists search for new probiotics to improve the rate of livestock production. The present study was aimed to isolate and evaluate breed-specific lactic acid bacteria (LAB) as potential animal probiotics. The current study was conducted during 10 months from July 2020 to April 2021, in which a total of n=12 strains were isolated from different samples including milk, rumen, and feces of Nilli Ravi Buffaloes. These isolates were evaluated for their antimicrobial potential against common animal pathogens (Bacillus spp., E. coli, Staphylococcus aureus, Salmonella spp., Listeria spp.). All the isolates were identified using 16S rRNA gene sequencing and the phylogenetic analyses inferred that these strains showed close relations to the species of various genera; Enterococcus lactis, Pediococcus pentosaceus, Bacillus subtilis Weissella cibaria, Weissella soli, Bacillus tequilensis, Weissella bombi, Bacillus licheniformis, Lactococcus lactis, Bacillus megaterium, Lactobacillus ruminis, and Lactococcus lactis. NMCC-Ru2 has exhibited the enormous potential of antimicrobial activity, 28 mm, for Salmonella typhimurium;23 mm for Listeria monocytogenes 21 mm for E.coil. Highest resistance was seen in NMCC-Ru2 agasint test antbiotic, like 25.5 mm for Tetracycline. Overall results revesl that the probiotic profile of isolates was achieved using standard criteria, particularly with animal probiotic properties

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Section: Listeria Monocytogenesmentioning

confidence: 92%

Tracing probiotic producing bacterial species from gut of buffalo (Bubalus bubalis), South-East-Asia

et al. 2024

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“…Ox-gall contains unconjugated and conjugated bile salts, including glycocholic acid, taurocholic acid, cholesterol, and lecithin. A strain is considered to be an efficient probiotic when it is able to withstand 0.2–0.6% bile salt, which resembles the environment of human intestinal bile salt 47 . In our study, MKL8 demonstrated strong tolerance to all bile salt concentrations, indicating its essential ability to thrive in the gastrointestinal tract (GIT) by resisting bile salts.…”

Section: Discussionmentioning

confidence: 99%

Probiotic evaluation, adherence capability and safety assessment of Lactococcus lactis strain isolated from an important herb “Murraya koenigii”

Shivani,

Sathiavelu

2024

Sci Rep

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Lactic acid bacteria (LAB) isolated from medicinal herb Murraya koenigii, commonly known as curry leaf, which promotes the growth and maintenance of gut microbiota, were studied for their probiotic potential. The key objective of this research was to isolate and evaluate probiotic characteristics, test adherence capabilities, and confirm their safety. Lactococcus lactis (MKL8), isolated from Murraya koenigii, was subjected to in vitro analysis to assess its resistance to the gastric environment, ability to adhere Caco-2 cells, anti-microbial activity, hydrophobicity, auto-aggregation, and safety profiling through MTT assay and hemolytic. MKL8 exhibited growth at 0.5% phenol concentrations (> 80%) and was able to survive in conditions with high bile concentrations (> 79%) and a relatively low pH (72%-91%). It shows high tolerance to high osmotic conditions (> 73%) and simulated gastric juice (> 72%). Additionally, MKL8 demonstrated strong hydrophobicity (85%), auto-aggregation (87.3%-91.7%), and adherence to Caco-2 cells. Moreover, it had an inhibitory effect against pathogens too. By performing the hemolytic and MTT assays, the non-toxicity of MKL8 isolate was examined, and it exhibited no harmful characteristics. Considering MKL8's resistance to gastrointestinal tract conditions, high surface hydrophobicity, non-toxicity, and ability to inhibit the tested pathogens, it can be concluded that MKL8 demonstrated promising probiotic properties and has potential for use in the food industry.

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“…The genera Lactobacillus and Bifidobacterium include the largest number of probiotic strains, although many studies have reported the probiotic properties of several Lactococcus lactis strains [ 21 , 22 , 23 , 24 ]. Due to its historical use in food fermentation, Lactococcus lactis is “generally recognized as safe” (GRAS) and has been proposed as a possible vehicle to deliver therapeutic molecules in the gastrointestinal tract [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

Probiotic Properties of Lactococcus lactis Strains Isolated from Natural Whey Starter Cultures

De Chiara,

Marasco,

Della Gala

et al. 2024

Foods

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Lactococcus lactis is a lactic acid bacterium (LAB), generally recognized as safe, and has been widely used in the food industry, especially in fermented dairy products. Numerous studies have evaluated the technological and probiotic properties of lactococci; however, few studies have reported the probiotic characteristics of L. lactis strains isolated from dairy products. In this work, probiotic potential, including survival in simulated gastric juice, tolerance to bile salts, hydrophobicity, and auto- and co-aggregation, was evaluated in L. lactis strains from natural whey starter cultures. The results highlighted the potential probiotic properties of some strains under study, which showed high values of hydrophobicity and auto-aggregation and low values of co-aggregation with the tested pathogenic strains. In addition, studies of safety parameters, such as antibiotic susceptibility and haemolytic activity, confirmed the safety status of all strains under study. Finally, the four most promising strains were investigated for their ability to inhibit the enteroinvasive Escherichia coli (EIEC) and Salmonella Typhimurium adhesion to epithelial cells, using a model of co-cultured epithelial cells. The results demonstrated that L. lactis strains A3-A5-I4-I7 showed the ability to compete with pathogens as well as the ability to exert a protective effect on cells previously infected with E. coli or S. Typhimurium. The identification of new probiotic LAB strains from dairy products aims to produce novel functional foods.

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Probiotic and Antioxidative Properties ofL. LactisLL27 Isolated from Milk

Cited by 7 publications

References 56 publications

Tracing probiotic producing bacterial species from gut of buffalo (Bubalus bubalis), South-East-Asia

Tracing probiotic producing bacterial species from gut of buffalo (Bubalus bubalis), South-East-Asia

Probiotic evaluation, adherence capability and safety assessment of Lactococcus lactis strain isolated from an important herb “Murraya koenigii”

Probiotic Properties of Lactococcus lactis Strains Isolated from Natural Whey Starter Cultures

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