Antioxidative potential of folate producing probiotic Lactobacillus helveticus CD6

Abstract

Folate producing Lactobacillus sp. CD6 isolated from fermented milk showed 98% similarity with Lactobacillus helveticus based on 16S rRNA gene sequence analysis. It was found to produce a folic acid derivative 5-methyl tetrahydrofolate (5-MeTHF). The intracellular cell-free extract of strain demonstrated antioxidative activity with the inhibition rate of ascorbate autoxidation in the range of 27.5%±3.7%. It showed highest metal ion chelation ability for Fe 2+ (0.26±0.06 ppm) as compared to Cu 2+. The DPPH (α,α-Diphenyl-β-Picrylhydrazyl) radical scavenging activity for intact cells were found to be 24.7%±10.9% proved its antioxidative potential. Furthermore, it demonstrated 14.89% inhibition of epinephrine autoxidation, 20.9±1.8 μg cysteine equivalent reducing activity and 20.8%±0.9% hydroxyl radical scavenging effect. The strain was evaluated for probiotic properties as per WHO and FAO guidelines. It showed 90.61% survival at highly acidic condition (pH 2.0), 90.66% viability in presence of synthetic gastric juice and 68% survivability at 0.5% bile concentration for 24 h. It was susceptible to many antibiotics which reduces the prospect to offer resistance determinants to other organisms if administered in the form of probiotic preparations. It showed in vitro mucus binding and antimicrobial activity against enteric pathogens like Salmonella typhimurium (NCIM 2501), Streptococcus pyogenes (NCIM 2608), and Staphylococcus aureus (NCIM 5021) and moreover it showed nonhemolytic activity on sheep blood agar.

Preparation, characterization and in vitro antioxidative potential of synbiotic fermented dairy products

“…Free hydroxyl radicals are extremely reactive by either removing hydrogen from or adding hydroxyl radicals to biological entities and are also involved in several pathological conditions as well as known for lipids peroxidation and DNA damages (Halliwell and Chirico 1993). Hydroxyl radicals are formed through Fenton reaction by using iron (Ahire et al 2013). DPPH radical scavenging activity of both synbiotic products ( Fig.…”

“…The activity was comparable to present results. Ahire et al (2013) studied antioxidative activity of intact cells and ICFE of L. helveticus CD6. The DPPH activity of intact cells was found to be 24.7 ± 10.9 %, more than the cell extracts (6.8 ± 2.0 %).…”

“…Antioxidants in food are of interest because of their ability to protect components of the food itself against oxidative damage (Halliwell et al 2000). Besides the probiotic properties, there are some reports of the antioxidative potentials of lactic acid bacteria, including starter cultures (Ahire et al 2013;Kullisaar et al 2002;Achuthan et al 2012;Lin and Chang 2000). But very few reports are available on antioxidative potential of probiotics and synbiotic products.…”

Probiotic attributes, antioxidant, anti-inflammatory and neuromodulatory effects of Enterococcus faecium CFR 3003: in vitro and in vivo evidence

“…They cause cellular oxidative damage that is related to many diseases (Ahire et al, 2013). DPPH is a relatively stable organic radical that has been widely used in the determination of the antioxidant activities of probiotic cell-free extracts (Afify et al, 2012).…”

Growth promoting effects of some lichen metabolites on probiotic bacteria

“…Many of the lichen extracts and their metabolites are reported to have variety of biological actions including antioxidative potential (Müller 2001;. Very recently Ahire et al (2011) reported that antioxidant activity of intracellular cell-free extract of a Lactobacillus species. In our study supplementation of lichen metabolites significantly enhanced the growth of L. casei.…”