Free DNA precipitates calcium phosphate apatite crystals in the arterial wall in vivo

Coscas, Raphaël; Bensussan, Marie; Jacob, Marie‐Paule; Louedec, Liliane; Massy, Ziad A.; Sadoine, Jérémy; Daudon, Michel; Miller, Catherine; Bazin, Dominique; Michel, Jean‐Baptiste

doi:10.1016/j.atherosclerosis.2017.03.005

Cited by 42 publications

(28 citation statements)

References 37 publications

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“…But calcifications could also illustrate more cell deaths. Our observation of exact co-localization of calcifications with free DNA in this model confirms our precedent results that free DNA could serve of phosphate matrix for ionized calcium precipitation and hydroxyapatite crystal formation [37]. Calcifications have been reported in both morphologies of aneurysms in human and reported to be both protective [36] and pathogenic [38].…”

Section: Discussionsupporting

confidence: 89%

Persistence of Intraluminal Thrombus Makes Saccular Aneurysm More Biologically Active than Fusiform in an Experimental Rat Model

et al. 2020

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Introduction: Saccular aneurysms are thought to have a worse prognosis than fusiform aneurysms in humans, due to hemodynamic reasons. However, data comparing hemodynamic and biology in saccular and fusiform aneurysms are lacking. The main objective was to evaluate the impact of aneurysm morphology on intra-luminal thrombus (ILT) formation and activity. Methods: Forty Lewis rats were randomly divided into 2 groups of 20: "saccular" (Group A) and "fusiform" (Group B) aneurysms. Decellularized thoracic aortas from guinea pigs were xenografted to create saccular or fusiform aneurysms. Final imaging evaluation of the aneurysms was carried out during the third week, by quantitative Doppler ultrasound and magnetic resonance imaging. As-says of myeloperoxidase (MPO), platelet factor 4 (PF4), advanced oxidation protein products (AOPPs) iron and matrix metallopeptidase-9 (MMP-9) were performed as biological criteria. Results: Quantitatively, saccular aneurysms are characterized by a more thicker ILT, lower inflow velocities and more important relative backflow velocities as compared to fusiform aneurysms. Compared to fusiform, saccular aneurysms released significantly more MPO (p = 0.004), PF4 (p = 0.02), AOPPs (p < 0.002), iron (p < 0.0001) and MMP-9 (p < 0.04). Conclusion: Experimental saccular and fusiform aneurysms show differential specific hemodynamics, which seem to impact the histology and the biology of the ILT in each type of aneurysm.

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Section: Discussionsupporting

confidence: 89%

Persistence of Intraluminal Thrombus Makes Saccular Aneurysm More Biologically Active than Fusiform in an Experimental Rat Model

et al. 2020

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“…Once in the extracellular environment, some nucleic acids may have a different function in addition to their intracellular roles. For instance, nucleic acids have pronounced affinity for apatite mineral, 24 and may participate in biomineralization, 25 as calcium sequestering and mineral templating scaffolds, a role that is already proposed for structurally related polynucleotides like poly-ADP-ribose. 4 Phospholipids 26 are abundant in mineralized bone.…”

Section: N Dnpmentioning

confidence: 97%

Detection of nucleic acids and other low abundance components in native bone and osteosarcoma extracellular matrix by isotope enrichment and DNP-enhanced NMR

Goldberga

Chow

et al. 2019

RSC Adv.

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“…The DNA extraction method selected must be adapted for bacterial DNA extraction and to the specificity of the human tissue. Calcifications are a common macroscopic feature of atherothrombotic samples ( Figure S1), which prevent optimal DNA extraction because they saturate and block the extraction column filter or interfere with free DNA [41] or extraction reagents. This technical point is not addressed in the literature, and details are lacking on how to adjust for this limitation when using the DNA extraction kits (Table 3), while it is very important to limit false negative due to low yield.…”

Section: Dna Extraction From Atherothrombotic Tissuesmentioning

confidence: 99%

Innovative application of nested PCR for detection of Porphyromonas gingivalis in human highly calcified atherothrombotic plaques

Brun

Rangé

Prouvost

et al. 2020

Journal of Oral Microbiology

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Atherothrombosis, leading to stroke and myocardial infarction, is responsible for most of the deaths in the world. An increased risk of atherothrombotic vascular events has been reported in patients with periodontitis. Periodontitis is a chronic multifactorial inflammatory disease, which involves a dysbiotic microbiota, and leads to a progressive destruction of the tooth-supporting apparatus. Transcient periodontal pathogen blood translocation, mainly bacteremia, has been associated with the severity of gingival inflammation. The identification of periodontal bacteria within atherothrombotic plaques is challenging and unpredictable. This review aims to summarize existing molecular technics for identifying periodontal microbiota in human atherothrombotic samples. A secondary objective is to describe a protocol for the identification of Porphyromonas gingivalis from highly calcified, atherothrombotic human samples that is based on our experience in translational cardiovascular research. Compared to direct real-time PCR, our protocol based on nested PCR has increased the detection of Porphyromonas gingivalis by 22.2% with good specificity. ARTICLE HISTORY

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Free DNA precipitates calcium phosphate apatite crystals in the arterial wall in vivo

Abstract: This translational study demonstrates that free DNA could be involved in arterial calcification formation by precipitating calcium phosphate apatite crystals in the vessel wall.

Cited by 42 publications

References 37 publications

Persistence of Intraluminal Thrombus Makes Saccular Aneurysm More Biologically Active than Fusiform in an Experimental Rat Model

Persistence of Intraluminal Thrombus Makes Saccular Aneurysm More Biologically Active than Fusiform in an Experimental Rat Model

Detection of nucleic acids and other low abundance components in native bone and osteosarcoma extracellular matrix by isotope enrichment and DNP-enhanced NMR

Innovative application of nested PCR for detection of Porphyromonas gingivalis in human highly calcified atherothrombotic plaques

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