Free Calvarial Periosteum Graft Vascularized by an Omental Flap in a Rat Model

Abstract

Because omental flaps are useful for flap prefabrication and the cambium layer of the periosteum can be osteogenic, we examined whether calvarial periosteum grafted onto greater omentum of rats was osteogenic and suitable for a flap. Distal omentum was wrapped with calvarial periosteum and so the cambium faced the omentum. Grafted omentum was harvested at 1 to 9 days. In other rats, grafted omentum was elevated as a pedicled flap and moved to the abdominal subcutis, to be harvested later at 1 to 5 months after the initial surgery. Bone formation was evaluated histologically, histochemically, and radiographically. On day 3, osteoid had formed. From day 4, calvarial periosteum was revascularized by omentum and bone was forming. New bone was maintained after grafting to subcutis for 5 months. Thus, bone formed by periosteum on the omentum could be used to reconstruct defects of the bone.

A Novel Method of Neo-osseous Flap Prefabrication: Induction of Free Calvarial Periosteum with Bioactive Glass

“…19 The osteogenic potential of free periosteal grafts was previously demonstrated in an omental flap prefabrication model. 20 Likewise, the use of bioactive glass as a tool of flap prefabrication is not a novel concept. In this experimental study, free periosteal graft and bioactive glass were used to create a vascularized prefabricated flap through vascular induction from a superficial epigastric artery and vein.…”

Viabilidade Do Enxerto De Omento-Maior Sobre O Osso in Vivo Em Ratos

“…onde foi injetado a fração lipídica do omento na córnea de coelhos, em resumo, a capacidade angiogênica precoce do omento já foi confirmada por estudos experimentais. No trabalho de Nazakato e outros(NAKAZATO et al, 2011b) é exibida a capacidade do retalho de omento suprir a vascularização do periósteo da calvária exposta de ratos, que em comparação ao músculo, tem ação superior.Os dois grupos também foram comparados quanto às freqüências da presença ou ausência macroscópica de tecido de granulação, hemorragia, necrose e pus, bem como a presença ou ausência ou quanto ao aumento, diminuição e característica fisiológica microscópica de fibroblastos, crosta, vasos trombosados, hemorragia, rejeição tecidual e vascularização local. Em todas estas comparações não foi possível demonstrar diferenças estatisticamente importantes entre os percentuais comparados.…”