Bone Tissue Engineering in the Greater Omentum is Enhanced by a Periosteal Transplant in a Miniature Pig Model

Naujokat, Hendrik; Lipp, Maximilian; Açil, Yahya; Wieker, Henning; Birkenfeld, Falk; Sengebusch, André; Böhrnsen, Florian; Wiltfang, Jens

doi:10.2217/rme-2018-0031

Cited by 12 publications

(9 citation statements)

References 46 publications

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“…Naujokat et al 11 performed a trial of bone tissue engineering using a scaffold implanted in the omentum. Bone scaffolds were soaked in bone marrow aspirate and bone morphogenetic protein and wrapped with either collagen membranes or autogenous periosteum.…”

Section: Prefabricated Flapsmentioning

confidence: 99%

Techniques and Innovations in Flap Engineering: A Review

Kouniavski

Egozi

Wolf

2022

Plastic and Reconstructive Surgery - Global Open

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Background: Currently, the gold standard for complex defect reconstruction is autologous tissue flaps, with vascularized composite allografts as its highest level. Good clinical results are obtained despite considerable obstacles, such as limited donor sites, donor site morbidity, and complex operations. Researchers in the field of tissue engineering are trying to generate novel tissue flaps requiring small or no donor site sacrifice. At the base of existing technologies is the tissue’s potential for regeneration and neovascularization. Methods: A review was conducted identifying relevant published articles in PubMed on the subject of flap engineering, with the focus on plastic surgery. This review article surveys contemporary technologies in flap engineering, including cell sheet technology, prefabricated flaps, and tissue engineering chambers. Conclusions: Some of the described procedures, though not yet ready for clinical use, are certainly ready for trial in large animal models and even human studies. Tissue engineering is a promising field for the handling of large and complex tissue defects.

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Section: Prefabricated Flapsmentioning

confidence: 99%

Techniques and Innovations in Flap Engineering: A Review

Kouniavski

Egozi

Wolf

2022

Plastic and Reconstructive Surgery - Global Open

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“…This approach is promising for augmenting the available rib bone stock; however, it is restricted by the size of the rib periosteum and involves a relative morbidity for rib segments harvesting. The second approach has involved nonvascularized periosteal grafts transplanted into the greater omentum of minipigs (Naujokat et al 2019(Naujokat et al , 2020. Although this approach violates the anterior abdominal wall, it has reflected the versatility of transplanting periosteal grafts into a distant IVB site for customized defect-specific bone tissue regeneration.…”

Section: Introductionmentioning

confidence: 99%

Periosteal Flaps Enhance Prefabricated Engineered Bone Reparative Potential

et al. 2021

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The clinical translation of bone tissue engineering for reconstructing large bone defects has not advanced without hurdles. The in vivo bioreactor (IVB) concept may therefore bridge between bone tissue engineering and reconstructive surgery by employing the patient body for prefabricating new prevascularized tissues. Ideally, IVB should minimize the need for exogenous growth factors/cells. Periosteal tissues are promising for IVB approaches to prefabricate tissue-engineered bone (TEB) flaps. However, the significance of preserving the periosteal vascular supply has not been adequately investigated. This study assessed muscle IVB with and without periosteal/pericranial grafts and flaps for prefabricating TEB flaps to reconstruct mandibular defects in sheep. The sheep ( n = 14) were allocated into 4 groups: muscle IVB (M group; nM = 3), muscle + periosteal graft (MP group; nMP = 4), muscle + periosteal flap (MVP group; nMVP = 4), and control group ( nControl = 3). In the first surgery, alloplastic bone blocks were implanted in the brachiocephalic muscle (M) with a periosteal graft (MP) or with a vascularized periosteal flap (MVP). After 9 wk, the prefabricated TEB flaps were transplanted to reconstruct a mandibular angle defect. In the control group, the defects were reconstructed by non-prevascularized bone blocks. Computed tomography (CT) scans were performed after 13 wk and after 23 wk at termination, followed by micro-CT (µCT) and histological analyses. Both CT and µCT analysis revealed enhanced new bone formation and decreased residual biomaterial volume in the MVP group compared with control and MP groups, while the M group showed less new bone formation and more residual biomaterial. The histological analysis showed that most of the newly formed bone emerged from defect edges, but larger areas of new bone islands were found in MP and MVP groups. The MVP group showed enhanced vascularization and higher biomaterial remodeling rates. The periosteal flaps boosted the reconstructive potential of the prefabricated TEB flaps. The regenerative potential of the periosteum was manifested after the transplantation into the mechanically stimulated bony defect microenvironment.

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“…In addition to the above deficiencies, the risk of varying degrees of immune response after autogenous bone transplantation needs to be assessed . Bone tissue engineering, which comprises seed cells, biological active factors, and signaling molecule scaffold materials, provides a new method for the reconstruction of bone defects . The key to successful bone defect repairing depends on the selection of seed cells .…”

Section: Introductionmentioning

confidence: 99%

“…8 Bone tissue engineering, which comprises seed cells, biological active factors, and signaling molecule scaffold materials, provides a new method for the reconstruction of bone defects. 9 The key to successful bone defect repairing depends on the selection of seed cells. 10 Mesenchymal stem cells (MSCs), with low immunogenicity and multidirectional differentiation potential, are regarded as ideal seed cells in tissue engineering.…”

Section: Introductionmentioning

confidence: 99%

BMP9 promotes osteogenic differentiation of SMSCs by activating the JNK/Smad2/3 signaling pathway

Zheng

Chen

Zhang

et al. 2019

J of Cellular Biochemistry

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Synovial mesenchymal stem cells (SMSCs) with high proliferation and multi differentiation ability, and low immunogenicity have attracted research attention for their potential application in tissue engineering. Once their ability of osteogenesis is strengthened, it will be of practical value to apply the SMSCs in the field of bone regeneration. The current study aimed to investigate the osteogenic characteristics of SMSCs induced by bone morphogenetic protein 9 (BMP9) both in vitro and in vivo and to elucidate the mechanism underlying these characteristics. Specifically, different BMPs were assessed to determine the protein that would be the most favorable for stimulating osteogenic differentiation of SMSCs following their separation. The BMP9‐enhanced osteogenesis of SMSCs was fully investigated in vitro and in vivo, and the c‐Jun N‐terminal kinase (JNK)/Smad2/3 signaling pathway stimulated by BMP9 was further explored. Our data suggested that BMP9 could significantly promote gene and protein expression of runt‐related transcription factor 2, alkaline phosphatase, osteopontin, and osteocalcin, and SP600125, a JNK‐specific inhibitor, could effectively decrease this tendency. Similar results were also confirmed in rats with cranial defects. In conclusion, our study indicated that BMP9 promotes bone formation both in vitro and in vivo possibly by activating the JNK/Smad2/3 signaling pathway.

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Bone Tissue Engineering in the Greater Omentum is Enhanced by a Periosteal Transplant in a Miniature Pig Model

Cited by 12 publications

References 46 publications

Techniques and Innovations in Flap Engineering: A Review

Techniques and Innovations in Flap Engineering: A Review

Periosteal Flaps Enhance Prefabricated Engineered Bone Reparative Potential

BMP9 promotes osteogenic differentiation of SMSCs by activating the JNK/Smad2/3 signaling pathway

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