Free amino acid profiling in grain Amaranth using LC–MS/MS

Nimbalkar, Mansingraj S.; Pai, Sandeep R.; Pawar, Nilesh V.; Oulkar, Dasharath; Dixit, G. B.

doi:10.1016/j.foodchem.2012.04.057

Cited by 78 publications

(49 citation statements)

References 23 publications

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“…Similar values were obtained by other authors except for the absence of proline, gamma aminobutyric acid, and ornithine but containing arginine and triptophane (Wang and Larkins 2001;Seebauer et al 2004). The results suggest that free amino acid determination in inbred lines studied could help the selection of genotype and may improve functional and nutritional qualities (Olesen, Meyer, and Stahnke 2004;Nimbalkar et al 2012). …”

supporting

confidence: 87%

“…Reliable and reproducible techniques for the identification and quantification of amino acids usually require derivatization (Nimbalkar et al 2012).The method involves extraction (Sarani et al 2013;Nimalaratne et al 2011;Pérez-Palacios et al 2014), purification, derivatization and analysis. Gas chromatography coupled with mass spectrometry (GC-MS) is an excellent technique for amino acid identification and quantitation (Pérez-Palacios et al 2014;Culea et al 2009;Culea, Horj, Iordache, and Florescu 2011;Culea, Horj, Iordache, and Cozar 2011;Hodisan et al 1998;Horj, Iordache, and Culea 2011;Iordache et al 2010;Bara et al 2010;Rö hlig, Eder, and Engel 2009).…”

Section: Introductionmentioning

confidence: 99%

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Determination of Amino Acids in Corn Seed by Gas Chromatography–Mass Spectrometry

Culea¹,

Scrob²,

Suvar³

et al. 2014

Analytical Letters

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A gas chromatography-mass spectrometry method was developed for the determination of free amino acids from corn inbred lines flour. Amino acids were extracted using 6% trichloroacetic acid. The extracts were purified using an ion-exchange solid phase, derivatized as trifluoroacetic butyl esters, and analyzed. The 15 N-methionine was used as the internal standard. The method was repeatable for most of the amino acids (coefficient of variation was in the order of 20%), and good accuracy and limits of detection were obtained. Among the free amino acids determined, the highest concentrations were obtained for aspartic acid, proline, gamma-aminobutyric acid, lysine, alanine, glutamic acid, and histidine. The study established the variation of the free amino acids within the inbred lines.

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supporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Determination of Amino Acids in Corn Seed by Gas Chromatography–Mass Spectrometry

Culea¹,

Scrob²,

Suvar³

et al. 2014

Analytical Letters

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“…[9,10] Techniques such as HPLC and LC-MS/MS are commonly used to analyze plant metabolites. [11][12][13] Several methods have been reported for determination of mangiferin, viz., high performance thin layer chromatography (HPTLC), high performance liquid chromatography (HPLC), ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS), capillary electrophoresis-mass spectroscopy (CE)-MS, and liquid chromatography-mass spectroscopy (LC)-MS from different sources. [14][15][16][17][18][19] Presence of mangiferin in S. chinensis has been reported earlier.…”

Section: Introductionmentioning

confidence: 99%

Optimization of Extraction Techniques and RP-HPLC Analysis of Antidiabetic and Anticancer Drug Mangiferin from Roots of ‘Saptarangi’ (Salacia chinensis L.)

Chavan¹,

Ghadage²,

Kshirsagar

et al. 2015

Journal of Liquid Chromatography & Related Technologies

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Saptarangi (Salacia chinensis L.) is a chief source of mangiferin, which has antidiabetic and anticancer properties. Extraction using continuous shaking extraction (CSE), ultrasonic extraction (UE), microwave assisted extraction (MAE), and steam bath assisted extraction (SBAE) were evaluated for extraction of mangiferin (MF) from roots of S. chinensis. The extracts were analyzed by reverse phase-high performance liquid chromatography (RP-HPLC) and Fourier transform-infra red spectroscopy (FT-IR) analysis. Operational parameters such as different solvents and exposure period were also evaluated. N,N-Dimethyl formamide (DMF 30%, v/v) extracted higher quantity of MF compared to other solvents. The results shows that the extraction of MF by SBAE was more efficient followed by MAE, CSE, and UE methods. Maximum quantity of MF (391.80 ppm) was obtained by SBAE technique and DMF (30%) as a solvent system. SBAE is the easiest and best method for screening of MF from S. chinensis. Furthermore, use of FT-IR validates the RP-HPLC technique for identification and quantification.

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“…Two major parameters affecting content yield of analytes, includes method of extraction and time required for it. There are a number of methods identified for extraction of plant based activities and metabolites (Pai et al, 2011a(Pai et al, ,b, 2015Murugan and Parimelazhagan, 2014;Nimbalkar et al, 2012;Patil et al, 2012;Pawar et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Effect of method and time of extraction on total phenolic content in comparison with antioxidant activities in different parts of Achyranthes aspera

Upadhya

Pai

Hegde

2015

Journal of King Saud University - Science

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The main purpose of this study was to evaluate the effect of extraction method with respect to time of exposure on total phenolic content and antioxidant potential of methanolic extracts (95%) from Achyranthes aspera leaves, stem and roots. Total phenolic content (TPC) was determined using the Folin-Ciocalteu method and antioxidant potential was tested using DPPH radical scavenging and FRAP assays. Plant populations aged 60 and 120 days were considered during the study. Overall, highest TPC was evident in mature plants (120 days) compared to younger (60 days). Similarly, leaves accumulated higher phenolics followed by roots and stem. Results showed the MAE technique to be efficient over USE and CSE methods. Same was evident in both the antioxidant assays tested. A similar trend was observed in both antioxidant assays as that of TPC, indicating phenolics to be the major contributor in the antioxidant potential of the plant. In conclusion it can be said that the yield of phenolic compounds depends on parameters viz. age of plant, part used for extraction, method of extraction and time required for the same. ª 2015 The Authors. Production and hosting by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Free amino acid profiling in grain Amaranth using LC–MS/MS

Cited by 78 publications

References 23 publications

Determination of Amino Acids in Corn Seed by Gas Chromatography–Mass Spectrometry

Determination of Amino Acids in Corn Seed by Gas Chromatography–Mass Spectrometry

Optimization of Extraction Techniques and RP-HPLC Analysis of Antidiabetic and Anticancer Drug Mangiferin from Roots of ‘Saptarangi’ (Salacia chinensis L.)

Effect of method and time of extraction on total phenolic content in comparison with antioxidant activities in different parts of Achyranthes aspera

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