Effect of method and time of extraction on total phenolic content in comparison with antioxidant activities in different parts of Achyranthes aspera

Upadhya, Vinayak; Pai, Sandeep R.; Hegde, Harsha V.

doi:10.1016/j.jksus.2015.04.004

Cited by 34 publications

(26 citation statements)

References 24 publications

(20 reference statements)

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“…In the present study, plant part and method of extraction were found to be the most important factors in extraction optimization, however time of extraction least affected variation of yield of TPC as well as TFC. Recently, similar observations were made for Clinacanthus nutans [28] and Achyranthes aspera [29] . It can be noted that, TPC and TFC content in the studied plant parts differ significantly from each other and the contents of TPC, and TFC was higher in roots than other plant parts.…”

Section: Resultssupporting

confidence: 76%

Extraction efficiency, phytochemical profiles and antioxidative properties of different parts of Saptarangi ( Salacia chinensis L.) – An important underutilized plant

Ghadage¹,

Kshirsagar

Pai

et al. 2017

Biochemistry and Biophysics Reports

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The study aimed to evaluate extraction efficiency, detection and quantification of phytochemicals, minerals and antioxidative capacity of different parts of Salacia chinensis L. Continuous shaking extraction, steam bath assisted extraction, ultrasonic extraction and microwave assisted extraction with varied time intervals were employed for extraction of phenolics, flavonoids, and antioxidants. Preliminary screening revealed the presence of wide array of metabolites along with carbohydrates and starch. Steam bath assisted extraction for 10 min exposure was found most suitable for extraction phenolics (46.02 ± 2.30 mg of gallic acid equivalent per gram of dry weight and 48.57 ± 2.42 mg of tannic acid equivalent per gram of dry weight) and flavonoids (35.26 ± 1.61 mg of quercetin equivalent per gram of dry weight and 51.60 ± 2.58 mg of ellagic acid equivalent per gram of dry weight). In support, reverse phase-high performance liquid chromatography- diode array detector confirmed the presence of seven pharmaceutically important phenolic acids. Antioxidant capacity was measured by 1, 1- diphenyl-1-picryl hydrazyl (DPPH), ferric reducing antioxidant power (FRAP), 2, 2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) scavenging (ABTS) and N, N-dimethyl-p-phenylenediamine (DMPD) assays and represented as trolox equivalent antioxidant capacity (TEAC) and ascorbic acid equivalent antioxidant capacity (AEAC). Antioxidant capacity ranged from 121.02 ± 6.05 to 1567.28 ± 78.36 µM trolox equivalent antioxidant capacity and 56.62 ± 2.83 to 972.48 ± 48.62 µM ascorbic acid equivalent antioxidant capacity. Roots showed higher yields of illustrated biochemical parameters, however fresh fruit pulp was found a chief source of minerals. Gas chromatography-mass spectroscopic analysis revealed the presence of a vast array of phytoconstituents associated with different plant parts. The present study revealed the amounts of minerals and diverse phytoconstituents in various parts of S. chinensis and confirmed its medicinal and nutritional implications.

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Section: Resultssupporting

confidence: 76%

Extraction efficiency, phytochemical profiles and antioxidative properties of different parts of Saptarangi ( Salacia chinensis L.) – An important underutilized plant

Ghadage¹,

Kshirsagar

Pai

et al. 2017

Biochemistry and Biophysics Reports

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“…3. Conventional methods like CSE and SBAE have been traditionally used for extraction of terpenes, phenolics and other antioxidant compounds 16,17 .…”

Section: Scientific Reports |mentioning

confidence: 99%

“…These enable us in obtaining a better quality and high efficiency of targeted yield from the plant. Hence, one has to optimize the recovery of the metabolites using extraction methods 16,17 .…”

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confidence: 99%

Efficient extraction of cucurbitacins from Diplocyclos palmatus (L.) C. Jeffrey: Optimization using response surface methodology, extraction methods and study of some important bioactivities

Patel

Attar

Sakate

et al. 2020

Sci Rep

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Diplocyclos palmatus (L.) C. Jeffrey is an important medicinal plant used in several reproductive medicines. It serves as a wide source of tetracyclic triterpens called cucurbitacins. Response surface methodology (RSM) with Box-Behnken design (BBD) was studied to optimize the production of cucurbitacins. RSM put forth the ideal conditions such as 1:30 SS ratio (g/mL), 80 rpm (mixing extraction speed), 150 µm mean particle size, 30 min extraction time and 50 °C using chloroform in continuous shaking extraction (CSE) and showed the highest cucurbitacin I (CUI) content (2.345 ± 0.1686 mg/g DW). Similarly, the highest yield of cucurbitacin B (CUB) (1.584 ± 0.15 mg/g DW) was recorded at ideal conditions (1:40 g/mL SS ratio and 60 min time and others similar to CUI). Among the tested extraction methods, the highest CUI, CUB, and CUI + B yield (1.437 ± 0.03, 0.782 ± 0.10, 2.17 ± 0.35 mg/g DW, respectively) as well as promising DPPH radical scavenging activity (25.06 ± 0.1 µgAAE/g DW) were recorded from the SBAE (steam bath assisted extraction). In addition, MAE and UAE revealed the highest inhibition of α-amylase (68.68%) and α-glucosidase (56.27%) enzymes, respectively. Fruit extracts showed potent anticancer activity against breast (MCF-7) and colon (HT-29) cancer cell lines (LC 50 -44.27 and 46.88 µg/mL, respectively). Our study proved that SS ratio, particle size and temperature were the most positively influencing variables and served to be the most efficient for the highest recovery of CUI and CUB. Based on the present study, the fruits of D. palmatus were revealed as a potent antioxidant, anti-diabetic and anticancer bio-resource that could be explored further to develop novel drug to manage diabetes, cancer and oxidative stress related disorders.The plants from the family Cucurbitaceae are commonly called as melons, squashes, and gourds which are traditionally used in the human diet. It comprises 122 genera and 940 species of which 31 genera and 94 species are found in India 1 . Diplocyclos palmatus (L.) C. Jeffrey is a slender-stemmed tendril climber commonly called as Shivlingi. Traditionally, this plant has been used in the folk medicine and possesses several activities such as gynaecological, anti-asthmatic, anti-convulsant, anti-venom, anti-inflammatory, androgenic and antioxidant 2-4 . The family Cucurbitaceae has been recognized as a rich source of bitter compounds, called cucurbitacins 5 . The cucurbitacins are highly unsaturated triterpenes containing many keto-, hydroxy-and acetoxy-groups. They are the major active components in traditional medicine, herbal remedies and Pharmacopoeia to treat various health issues such as hepatoprotective, anti-fertility, diabroticites cardiovascular and anti-inflammatory activities 6 . In a recent study, cucurbitacin I (CUI) has been identified as a strong inhibitor of the JAK2/STAT3 signaling pathway (a common oncogenic signaling pathway), which is constitutively activated in many types of cancer; hence, it is considered as a milestone in cancer therapy 7 . Similarly...

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“…Extraction is the first important step in the recovery and purification of active substrates from biological materials (Tan (Spigno et al, 2007). Moreover, the extraction method and the extraction time are two of the most important factors in the extraction process (Upadhya et al, 2015). Different methods have been used to extract ant alarm pheromones.…”

Section: Efficiency Of Extraction Of Alarm Pheromone Component Relatementioning

confidence: 99%

Intra- and inter-specific variation in alarm pheromone produced bySolenopsisfire ants

Balusu

Zhang

et al. 2017

Bull. Entomol. Res.

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Some fire ants of the genus Solenopsis have become invasive species in the southern United States displacing native species by competition. Although the displacement pattern seems clear, the mechanisms underlying competitive advantage remain unclear. The ability of ant workers to produce relatively larger amount of alarm pheromone may correspond to relative greater fitness among sympatric fire ant species. Here we report on quantitative intra-specific (i.e. inter-caste) and inter-specific differences of alarm pheromone component, 2-ethyl-3,6-dimethylpyrazine (2E36DMP), for several fire ant species. The alarm pheromone component was extracted by soaking ants in hexane for 48 h and subsequently quantified by gas chromatography-mass spectrometry at single ion monitoring mode. Solenopsis invicta workers had more 2E36DMP than male or female alates by relative weight; individual workers, however, contained significantly less pyrazine. We thus believe that alarm pheromones may serve additional roles in alates. Workers of Solenopsis richteri, S. invicta, and hybrid (S. richteri × S. invicta) had significantly more 2E36DMP than a native fire ant species, Solenopsis geminata. The hybrid fire ant had significantly less 2E36DMP than the two parent species, S. richteri and S. invicta. It seems likely that higher alarm pheromone content may have favored invasion success of exotic fire ants over native species. We discuss the potential role of inter-specific variation in pyrazine content for the relationship between the observed shifts in the spatial distributions of the three exotic fire ant species in southern United States and the displacement of native fire ant species.

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Effect of method and time of extraction on total phenolic content in comparison with antioxidant activities in different parts of Achyranthes aspera

Cited by 34 publications

References 24 publications

Extraction efficiency, phytochemical profiles and antioxidative properties of different parts of Saptarangi ( Salacia chinensis L.) – An important underutilized plant

Extraction efficiency, phytochemical profiles and antioxidative properties of different parts of Saptarangi ( Salacia chinensis L.) – An important underutilized plant

Efficient extraction of cucurbitacins from Diplocyclos palmatus (L.) C. Jeffrey: Optimization using response surface methodology, extraction methods and study of some important bioactivities

Intra- and inter-specific variation in alarm pheromone produced bySolenopsisfire ants

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