Fraksi Kloroform Ekstrak Buah Mentimun (Cucumis sativus L.) Sebagai Anti Bakteri terhadap Staphylococcus epidermidis

Abstract

Chloroform Fraction of Extract Cucumber Fruit (Cucumis sativus L.) as of Antibacterial Staphylococcus epidermidis. The use of antibiotics in the community massively trigger resistance to bacteria, to that need for research on other alternatives, especially herbal medicine as an antibacterial. One of the medicinal plants are cucumbers that are widely available in Indonesia. The purpose of this study was to scientifically prove the antibacterial activity of chloroform fraction cucumber extract against Staphylococcus epidermidis. Extraction cucumber done by maceration, then extract fractionated with chloroform. The fraction was used the concentration of 5%, 10%, 15%, 20% and 25%. Then tested the antibacterial power and determination of the MIC. Results of test antibacterial of the extract chloroform of cucumber against S. epidermidis showed a zone of inhibition at concentrations of 5%, 10%, 15%, 20%, 25% diameter respectively 5.86 mm, 6.5 mm, 7.05 mm, 7.43 mm, and 9.92 mm, in addition to it tested the determination of minimum inhibitory concentration, minimum inhibitory concentrations of chloroform fraction extract cucumber fruit against S. epidermidis is at a concentration of 3%. The results obtained from the culture media growth on NA which means the fraction of the chloroform extract of cucumber in 3% is bacteriostatic. Keywords: Cucumber fruit, Staphylococcus epidermidis, Chloroform Abstrak: Fraksi Kloroform Ekstrak Buah Mentimun (Cucumis sativus L.) Sebagai Antibakteri terhadap Staphylococcus epidermidis.Penggunaan antibiotik secara besar-besaran di masyarakat memicu terjadinya resistensi terhadap bakteri, Untuk itu perlu adanya penelitian tentang alternatif lain terutama obat herbal sebagai antibakteri. Satu di antara tanaman berkhasiat obat adalah mentimun yang banyak terdapat di Indonesia.Tujuan penelitian ini adalah untuk membuktikan daya antibakteri fraksi kloroform ekstrak buah mentimun terhadap Staphylococcus epidermidis. Ekstraksi buah mentimun dilakukan dengan cara maserasi dengan etanol 70%, selanjutnya ekstrak difraksinasi dengan kloroform. Fraksi yang digunakan adalah konsentrasi 5%, 10%, 15%, 20%, dan 25%. Kemudian dilakukan uji daya antibakteri dan penentuan KHM. Hasil penelitian uji daya antibakteri fraksi kloroform ekstrak buah mentimun terhadap bakteri Staphylococcus epidermidis menunjukan adanya zona hambat pada konsentrasi 5%, 10%, 15%, 20%, 25% dengan diameter masing-masing 5.86mm, 6.5mm, 7.05mm, 7.43mm dan 9.92mm. Selain itu, konsentrasi hambat minimum fraksi kloroform ekstrak buah mentimun terhadap S. epidermidis terdapat pada konsentrasi 3%. Dari hasil kultur ulang didapatkan pertumbuhan pada media NA yang berarti fraksi kloroform ekstrak buah mentimun pada konsentrasi 3% bersifat bakteriostatik.

Potensi Biofungisida Ekstrak Akar, Batang dan Daun Mentimun (Cucumis sativus L.) terhadap Fusarium oxysporum

“…Proses maserasi dilakukan selama 24 jam dengan pengadukan 6 jam sekali. Remaserasi dilakukan sebanyak tiga kali sampai diperoleh ekstrak yang kemudian akan diuapkan menggunakan evaporator dan oven dengan suhu 30 0 C sampai diperoleh ekstrak kental (Viogenta et al, 2017) .…”

Detection of Secondary Metabolites in Cucumber (Cucumis sativus) Leaves and Its Potential as Candidates for Acne Drug Using Histochemical Analysis and In Silico Study

“…One of the plants that has been proven to be efficacious as an acne medicine is cucumber (Cucumis sativus). Apart from being consumed by the public, cucumbers are also widely used to cure diseases, one of which is acne [5], previous studies have explained a lot about cucumber as an acne medicine, but there is still little research that discusses the benefits of the leaves. Phytochemical screening of cucumber leaf chloroform extract contains alkaloids, glycosides, steroids, flavonoids, saponins, and tannins [6], based on the results of the study, there were several phenolic compounds identified in cucumber leaves including cucurbit-asides B, cucurbit-asides C, and ferredoxin and also flavone glycosides such as isovitexin and saponarin which can act as antiinflammatory, anti-allergic, antimicrobial and antiviral.…”