Fragmented Fat: A new method for harvesting and processing of lipograft

Mendes, Flávio Henrique; Viterbo, Fausto; Deffunne, Elenice; Domingues, Maria Aparecida Custódio; Golim, Márjorie de Assis; Gabas, José Marcos; Rossoni, Renan Roldi; Nunes, Helga Caputo

doi:10.1016/j.bjps.2019.02.001

Cited by 3 publications

(5 citation statements)

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“…This fragmentation process has been previously published and scientifically validated by in vivo and in vitro methodological studies that found no statistically significant difference in biological behavior between fragmented fat and regular lipoaspirate. 8,9…”

Section: Methodsmentioning

confidence: 99%

“…This fragmentation process has been previously published and scientifically validated by in vivo and in Plastic and Reconstructive Surgery • March 2022 vitro methodological studies that found no statistically significant difference in biological behavior between fragmented fat and regular lipoaspirate. 8,9 Details of the fragmentation device and process, as well as the grafting surgical technique, are widely demonstrated within our Video, which provides a complete case description. [See Video (online), which shows a full demonstration of the fragmentation device, including assembly and function, as well as its clinical application, with gluteal autoaugmentation by means of fragmented fat transfer during a belt lipectomy.]…”

Section: Fragmentation Techniquementioning

confidence: 99%

“…no statistically significant difference between fragmented fat and syringe lipoaspirate. 8,9 The purpose of this study was to assess the initial clinical evidence on fragmented fat transfer to restore volume and enhance deflated gluteal contour in a sample of massive weight loss patients.…”

Section: Disclosure: the Authors Have No Financial Interest To Declar...mentioning

confidence: 99%

“…Those findings were scientifically validated by two previous publications describing in vitro methods (histologic analysis, immunohistochemical analysis, trypan blue testing, flow cytometric analysis, and cell culturing) and in vivo methods (mass, volume, and histologic analysis of xenografts in mice) that found no statistically significant difference between fragmented fat and syringe lipoaspirate. 8,9…”

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confidence: 99%

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Fragmented Fat Transfer for Massive Weight Loss Gluteal Contouring

Mendes

Viterbo

2022

Plastic &Amp; Reconstructive Surgery

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Summary: A recently introduced novel fragmentation technique has made it possible to convert fat tissue from apronectomy into large-scale viable lipograft, providing a glimpse of an alternative to traditional liposuction and its harvesting limitations. The purpose of this study was to assess the initial clinical evidence on fragmented fat transfer to restore volume and enhance deflated gluteal contours in a sample of massive weight loss patients. The authors retrospectively reviewed medical records of 20 women who received autologous buttock fat grafts that were harvested and fragmented following belt lipectomies and/or fleur-de-lis abdominoplasties. The average total volume subcutaneously grafted into the buttocks was 720 ml (bilateral), ranging from 500 ml to 960 ml. No local gluteal complications, such as seroma, infection, fat necrosis, or subcutaneous nodulations, were reported. Photographic observation of postoperative results over a 6- to 14-month follow-up period showed clear enhancement of body contouring in all patients, especially in those in whom fat transfer was combined with circumferential lifts. Fat fragmentation has proved useful and effective in providing great amounts of viable fat graft in a safe, simple, and reproducible technique without noticeable complications. The method eliminates the organic response to the insult of liposuction while using the entirety of formerly discarded apronectomy tissue to provide fine, transferable, autologous fat without increasing operative time. Although preliminary, the authors’ clinical findings with this new technique support further studies of fragmented fat as an alternative to liposuction in gluteal fat grafting. Additional potential applications, including in wound healing and regenerative medicine, are worth exploring. CLINICAL QUESTION/LEVEL OF EVIDENCE: Therapeutic, IV.

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Section: Methodsmentioning

confidence: 99%

Section: Fragmentation Techniquementioning

confidence: 99%

Section: Disclosure: the Authors Have No Financial Interest To Declar...mentioning

confidence: 99%

mentioning

confidence: 99%

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Fragmented Fat Transfer for Massive Weight Loss Gluteal Contouring

Mendes

Viterbo

2022

Plastic &Amp; Reconstructive Surgery

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“…An undoubted advantage is that significant quantities of adipose panniculus are removed with lipectomy and liposuction in all post-bariatric surgery procedures. As previously described, dermolipectomy specimens can be used as sources of regenerative cellular components and processed in order to obtain autologous injectable grafts [55]. Instead of eliminating the removed fatty deposits, they can be fragmented by means of a special grinder and the material collected in 60-cc Luer-Lok syringes closed at both ends and then centrifuged for 3 min at 850 g. This phase of manipulation of the AT and the concentration of the cellular components necessary for the preparation of the regenerative cocktail is carried out while the surgery continues without lengthening operating time.…”

Section: Regenerative-assisted Post-bariatric Surgery (Rapbs)mentioning

confidence: 99%

New perspectives in regenerative medicine and surgery: the bioactive composite therapies (BACTs)

et al. 2021

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Regenerative medicine and surgery is a rapidly expanding branch of translational research in tissue engineering, cellular and molecular biology.To date, the methods to improve cell intake, survival, and isolation need to comply with a complex and still unclear regulatory frame, becoming everyday more restrictive and often limiting the effectiveness and outcome of the therapeutic choices. Thus, the authors developed a novel 360° regenerative strategy based on the synergic action of several new components called the bioactive composite therapies (BACTs) to improve grafted cells intake, and survival in total compliance with the legal and ethical limits of the current regulatory frame.The rationale at the origin of this new technology is based on the evidence that cells need supportive substrate to survive in vitro and this observation, applying the concept of translational medicine, is true also in vivo. Bioactive composite mixtures (BACMs) are tailor-made bioactive mixtures containing several bioactive components that support cells’ survival and induce a regenerative response in vivo by stimulating the recipient site to act as an in situ real bioreactor. Many different tissues have been used in the past for the isolation of cells, molecules, and growth factors, but the adipose tissue and its stromal vascular fraction (SVF) remains the most valuable, abundant, safe, and reliable source of regenerative components and particularly of adipose-derived stems cells (ADSCs). The role of plastic surgeons as the historical experts in all the most advanced techniques for harvesting, manipulating, and grafting adipose tissue is fundamental in this constant process of expansion of regenerative procedures. In this article, we analyze the main causes of cell death and the strategies for preventing it, and we present all the technical steps for preparing the main components of BACMs and the different mixing modalities to obtain the most efficient regenerative action on different clinical and pathological conditions. The second section of this work is dedicated to the logical and sequential evolution from simple bioactive composite grafts (BACGs) that distinguished our initial approach to regenerative medicine, to BACTs where many other fundamental technical steps are analyzed and integrated for supporting and enhancing the most efficient regenerative activity. Level of Evidence: Not gradable

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Frag Fat: Fragmented Fat for Lipografting

Mendes,

Viterbo

2023

Body Contouring

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Fragmented Fat: A new method for harvesting and processing of lipograft

Cited by 3 publications

References 3 publications

Fragmented Fat Transfer for Massive Weight Loss Gluteal Contouring

Fragmented Fat Transfer for Massive Weight Loss Gluteal Contouring

New perspectives in regenerative medicine and surgery: the bioactive composite therapies (BACTs)

Frag Fat: Fragmented Fat for Lipografting

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