Four new metabolites from the endophytic fungus Diaporthe lithocarpus A740

Natural Products from Endophytic Fungi Associated with Rubiaceae Species

“…(Pleosporaceae) isolated from medicinal plant M. officinalis produced two new metabolites, isobenzofuranone A 61 Their structures contain a 12-carbon atoms polyketide skeleton unit-linked, through an amide bond, with a derivative of L-isoleucine, a rare compound class [116]. The endophytic fungus D. pseudomangiferae retrieved from leaves of Sabicea cinerea species found along forest edges in the French Guiana, produces four metabolites: mycoepoxydiene (114) and altiloxin A (115), as well as enamidin (116) and eremofortin F (117) [117]. A filamentous fungus of the genus Diaporthe associated with the seeds of Cinchona ledgeriana, from West Java-Indonesia, produces cinchona alkaloids: quinine (118), quinidine (119), cinchonidine (120) and cinchonine (121), upon cultivation in a synthetic liquid medium [118][119][120][121][122].…”

“…Bioassay-guided fractionation of ethyl acetate extract from D. pseudomangifera, by cytotoxic effects against mammalian cancer cells allowed the isolation of the mycoepoxydien (114), which showed cytotoxic activity with IC 50 values of 7.5, 17.7, and 15.8 µM against against human uterine cervical carcinoma KB and MDA-MB-435 cells, respectively. The compound eremofortin F (117) was cytotoxic on KB (IC 50 = 13.9 µM) and MRC5 (IC 50 = 12.2 µM) cells [117].…”

“…The compounds isolated from the endophytic fungus T. koningiopsis were evaluated for cytotoxic activity against HepG-2, MCF-7, SF-268 and A549 cells lines. [114].…”

“…However, the compounds exhibited weak antibiotic activity with inhibition in concentrations above 250 μg mL −1 . The endophytic fungus, D. lithocarpus, yielded tenllone I ( 101 ), a new benzophenone derivative; two new eremophilane derivatives, lithocarins B ( 102 ) and C ( 103 ); a new monoterpenoid, lithocarin D ( 104 ); tenellone H ( 105 ); and phomopene ( 106 ) [ 114 ]. Studies of endophytic fungus of Nigerian medicinal plants led to isolation of multiforisin I ( 107 ) and 4-hydroxyphenylacetic acid ( 108 ) of Neurospora discreta (Sordariaceae) from leaves of M. lucida ( Figure 9 ) [ 115 ].…”

“…The compounds from D. lithocarpus , another endophyte also isolated from M. officinalis , were tested for their cytotoxic activity by the sulforhodamine B method on four human tumor cell lines (SF-268, MCF-7, HepG-2 and A549). The compounds lithocarin B ( 102 ), lithocarin C ( 103 ), and tenellone H ( 105 ) presented IC 50 values ranging from 30 to 100 μM in the four tumor cell lines selected [ 114 ].…”