Formulation Optimization, Characterization and in Vitro Anti-Cancer Activity of Curcumin Loaded Nanostructured Lipid Carriers

Abstract

Objective: The present study was aimed at preparing stable lyophilized curcumin loaded nanostructured lipid carriers (NLCs). The optimized lyophilized curcumin loaded NLCs were characterized and evaluated for various quality control parameters.
 Methods: The optimized curcumin loaded NLCs were prepared by modified hot emulsification using precirol ATO 5 (PRE), capmul MCM C8 EP (CAP) as solid and liquid lipids, respectively. The combination of tween 80 (T80) and solutol HS 15 (SHS) were used as an emulsifier. The NLCs dispersion was lyophilized into powder form to improve the thermodynamic stability of the formulation. The lyophilized curcumin loaded NLCs were evaluated for particle size, size distribution, zeta potential, entrapment efficiency (EE), drug loading, assay, in vitro drug release, crystallinity and surface morphology studies.
 Results: The optimized lyophilized curcumin loaded NLCs have a mean particle size of 286.2±11.5 nm with a size distribution of 0.288±0.011, a zeta potential of 0.247±0.025 mV with high entrapment of 98.20±1.53 % and drug loading of 2.50±0.21 %. The X-ray diffraction and endothermic peaks confirmed the maximum encapsulation of curcumin in lipid matrices. The particles were spherical with smooth surface morphology. In vitro release studies showed sustained release for up to 24 h. The cytotoxicity against human lung cancer line A-549 for curcumin-loaded NLCs was confirmed with positive control adriamycin (ADR).
 Conclusion: Curcumin-loaded NLCs prepared had a nanosize particle distribution with maximum entrapment efficiency. Dispersion stability was increased by the lyophilisation process. The solid lyophilized powder is reconstituted for oral delivery.

Lipid-Polymer Hybrid Nanoparticles for Topical Drug Delivery System

“…In contrast the second method, the drugs are conjugate to the LPHNP system with the help of hydrolyzable linker and this drug-conjugate linker gets hydrolyzed upon reaching the cancer cell resulting in the drug are released separately. According to Sengupta et al, the LPHNPs for multi-drug delivery containing an antiangiogenesis and another chemotherapeutic drug, resulting one inhibit the growth of tumor cells by cutting the blood supply to the cell and another kill the existing tumor cell 48 .…”

“…Ex. The delivery of Small interfering Ribonucleic acid to the cancer cells that initiate the RNA interference pathway to block the protein expression into the tumor initiation and progression 48 . The formulation method for developing a Small interfering Ribonucleic acid delivery system is some as a Deoxyribonucleic acid (DNA) delivery system (Polyplexes and Lipoplexes) 42 but Deoxyribonucleic acid (DNA) delivery has some limitations like poor stability during oral or systemic administration thus to overcome this problem develop a new system called Small interfering Ribonucleic acid (SiRNA) development.…”

Nanostructured Lipid Carriers for Transdermal Drug Delivery

“…In addition, AFM provides nanometer resolution structural, mechanical, and functional information about NLCs surfaces [46]. The NLCs usually appeared spherical in the TEM or SEM micrographs [47]. However, some studies found that the shape of NLCs was nonspherical, mainly cuboid.…”

Formulation and Optimization of Hydroxyurea Loaded Nanostructured Lipid Carriers Using Design of Experiment for the Effective Treatment of Ovarian Cancer

“…The NLC solution was first centrifuged for 20 min at 14000 rpm. The free drug content was then determined by diluting the supernatant solution and using UV spectrophotometry at wavelength 214 nm (15). DEE was calculated using the following equation: DEE % = Total HU taken − Free HU Total HU taken × 100…”

“…In cancer therapy, NLCs have recently emerged as a multifunctional platform for drug delivery. Many advantages of this delivery system have been reported in earlier research, including excellent entrapment efficiency, good stability, and sustained release of drugs at specific rhythmic intervals [12][13][14][15].…”