Formulation and Evaluation of Pectin-Calcium Chloride Beads of Azathioprine for Colon Targeted Drug Delivery System

Abstract

Objective: Calcium pectinate is an insoluble hydrophilic material used for sustained release delivery. The objective of the study was formulation and evaluation pectin-calcium chloride beads of azathioprine.Methods: Calibration curves of azathioprine were prepared in 0.1N HCl solution (pH-1.2) and phosphate buffer (pH-6.8 and pH-7.4). Fourier transform infrared spectroscopy and differential scanning calorimetry was used to determine compatibility between azathioprine and excipients. Formulation and optimization of calcium-pectinate beads were performed. The coating of the optimized batch was performed with eudragit S100. Micrometric properties, scanning electron microscopy, in vitro azathioprine release, and stability study was performed. Dissolution kinetic study was assessed for various kinetics models of the optimized batch. The Wilcoxon test followed by the Dunnett's multiple comparison tests were performed between uncoated and coated beads in vitro dissolution profile of optimized batch in 0.1N HCl after 2 h. Results:Fourier transform infrared spectroscopy and differential scanning calorimetry had identical peaks with that of pure azathioprine in compatibility study. Scanning electron microscopy of an uncoated optimized batch of beads indicated the smooth and uniform surface of prepared beads. Eudragit S100 coating was decreased release of azathioprine release in 0.1N HCl after 2 h (p = 0.049, q = 3.533). Korsmeyer/Peppa's model was applied with release exponent higher than 1. In vitro percentage cumulative azathioprine release was identical before and after stability study. Conclusion:Calcium pectinate beads of azathioprine, a multi-particulate dosage form using pH-dependent approaches were prepared.

Bipolymeric Pectin Millibeads Doped with Functional Polymers as Matrices for the Controlled and Targeted Release of Mesalazine

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Bioavailability and Dissolution Enhancement of Glyburide Nanosuspension

“…poloxamer 407 with formulation codes from GLY-1 to GLY-12. The best formulation had been identified and further subjected to both in vitro and in vivo studies [3][4][5]. The present study brought out the best formulation with an enhanced dissolution and bioavailability.…”

Formulation and in-vitro Evaluation of Vildagliptin Microspheres Using Pectin and Xanthan Gum as Polymers

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“…Fourier transform infrared spectroscopy (FTIR) study [11] The FTIR is a technique used to obtain an infrared spectrum of absorption of target material, that can be used to identify the functional groups and molecular structure. Pure vildagliptin and vildagliptin with a mixture of polymer FTIR spectra were recorded.…”