FORMAZAX FORMATION AS A MEASURE OF HORMONE INFLUENCE ON THE METABOLIC ACTIVITY OF RAT PROSTATE¹

THE MECHANISM OF <I>IN VIVO</I> VAGINAL TTC REDUCTION STIMULATED BY ESTROGEN IN SPAYED MICE

“…The ability of various tissues to reduce tetrazolium salts has been utilized as a parameter for the estimation of metabolic activity of the tissues responding to steroid hormones (Martin, 1960;Rosenkrantz et al, 1961;Schultze, 1964). Among these, it should be noticed that in vivo reduction of 2,3,5-triphenyltetrazolium chloride(TTC) in the vagina of ovariectomized mice, as described by Martin (1960), is a highly sensitive and specific index for the action of estrogen applied not only locally (Martin, 1960) but also systemically (Hori, 1964a), and thus considered as a useful tool for biochemical approach to an earlier reaction of the tissue to estrogen or anti-estrogen.…”