THE MECHANISM OF &lt;I&gt;IN VIVO&lt;/I&gt; VAGINAL TTC REDUCTION STIMULATED BY ESTROGEN IN SPAYED MICE

Hori, Takashi; Miyake, Tamotsu

doi:10.1507/endocrj1954.12.181

Cited by 2 publications

(3 citation statements)

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“…The results indicate that formazan particles are located mainly in the superficial cells and the density of deposition depends on the development of granular layer under there. This confirms the conclusion of previous reports (Hori andMiyake, 1964 and1965). These histological findings are roughly summarized in Table 2, showing that all the estrogen-induced early changes in vaginal histology are prevented by MER-25 administered simultaneously, whereas progesterone and testoterone affect rather later stage of estrogen action to inhibit the differentiation of epithelial cells, especially the keratinization.…”

Section: Resultssupporting

confidence: 91%

“…The site of TTC reduction in the vagina and its mechanism, have been clarified by Hori andMiyake (1964 and1965) who indicate that the TTC reduction takes place in the superficial cells and this is closely connected with the epithelial differentiation following the proliferation of basal cells which appears to be a morphological change primarily induced by estrogen stimulation. This is strongly supported also by the present experimentation showing that the difference between the 3 compounds MER-25, progesterone and testosterone, in their patterns of anti-estrogenic response manifests according to the time of injection when the vaginal TTC reduction is used as a criteria (Table 1).…”

Section: Resultsmentioning

confidence: 99%

“…In our previous port (Hori and Miyake, 1965), it was concluded that the estrogen-stimulated 2,3,5-triphenyltetrazolium chloride (TTC) reduction in vagina is an earlier transient phenomenon in the process of vaginal cornification, in which the superficial cells are brought into the anaerobic condition resulting from the rapid differentiation of epithelium. Further studies were conducted to see whether such an intimate relationship between the differentiation of vaginal epithelium and the increase of TTC reduction in superficial cells may exist even under the influences of anti-estrogenic substances, and to use of this phenomenon for the analysis of mode of action of various anti-estrogens.…”

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The Use of in Vivo Vaginal TTC Reduction of Spayed Mice for the Study of Mode of Action of Anti-Estrogens

Hori

Miyake

1966

Endocrinol Japon

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SYNOPSISThe study was made on the relationship between in vivo vaginal TTC reduction and the epithelial histology of mouse vagina under the influences of various antiestrogens such as MER-25, progesterone and testosterone. MER-25 markedly inhibited the estrogen-stimulated vaginal TTC reduction when administered simultaneously, but the inhibition was reduced or abolished according to the injection interval between estradiol and MER-25 being prolonged for 3, 6 or 12 hrs. Progesterone and testosterone, however, produced almost consistent inhibition without regard to the time of administration within a period of 12 hrs. after the estrogen injection. Histological investigations on the vaginal epithelium also demonstrated that MER-25 almost completely blocked an earlier response to estrogen, primary proliferation of epithelial cells, while progesterone and testosterone allowed the epithelial proliferation in a relatively normal manner.These results indicate that MER-25 only inhibits earlier step of estrogen action, whereas either progesterone or testosterone inhibits rather later stage. It is found that there is close correlation between the ability of formazan production in the superficial cells and differentiation of vaginal epithelium under influences of these anti-estrogenic substances, supporting the previous explanation for the mechanism of hormonal regulation of vaginal TTC reduction.

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Section: Resultssupporting

confidence: 91%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

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