Formation of interspecies fusants of Agaricus bisporus and Agaricus bitorquis mushroom by protoplast fusion

Singh, Rana Inder; Kanojiya, Aarti; Singh, Sandhu Sardul

doi:10.1007/s12088-007-0066-y

Cited by 8 publications

(4 citation statements)

References 10 publications

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“…The appressed colonized protoclones with growth rates of less than 0.96 mm/d exhibited a slow spawn run and failed to complete the spawn-running process, even after 45 days. These results also agreed well with the findings of Singh et al [41]. In this study, all the appressed protoclones produced structures like primordia in one or two out of three replicates.…”

Section: Colony Growth Rate and Spawn Runsupporting

confidence: 94%

Efficiency of RAPD and ISSR Markers in Differentiation of Homo- and Heterokaryotic Protoclones of Agaricus bisporus

Mahmudul

Bian²

2010

J. Microbiol. Biotechnol.

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Morphologically, nine different slow-growing protoclones were screened from regenerated protoplasts of heterokaryotic Agaricus bisporus. As such, the present study is the first report on differentiating homo-and heterokaryotic protoclones using random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers. Among 80 primers tested, the seven ISSR and seven RAPD primers selected for the analysis generated a total of 94 ISSR and 52 RAPD fragments, respectively. The ISSR fingerprinting also detected more polymorphic loci (38.29%) than the RAPD fingerprinting (34.61%). A principal coordinate analysis (PCA) was employed to evaluate the resolving power of the markers as regards differentiating protoclones. As a result, the mean polymorphism information content (PIC) for each marker system (i.e., 0.787 for RAPD and 0.916 for ISSR) suggested that ISSR is more effective for determining polymorphisms. The dendrograms constructed using RAPD, ISSR, and an integrated RAPD and ISSR marker system were highly correlated with one another as revealed by a high Mantel correlation (r= 0.98). The pairwise similarity index values also ranged from 0.64 to 0.95 (RAPD), 0.67 to 0.98 (ISSR), and 0.67 to 0.98 (RAPD and ISSR), whereas the mean similarity index values of 0.82, 0.81, and 0.84 were obtained for the RAPD, ISSR, and combined data, respectively. As there was a good correspondence between the RAPD and ISSR similarity matrices, ISSR would appear to be an effective alternative to RAPD in the genetic diversity assessment and accurate differentiation of homo-and heterokaryotic protoclones of A. bisporus.

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Section: Colony Growth Rate and Spawn Runsupporting

confidence: 94%

Efficiency of RAPD and ISSR Markers in Differentiation of Homo- and Heterokaryotic Protoclones of Agaricus bisporus

Mahmudul

Bian²

2010

J. Microbiol. Biotechnol.

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“…Appressed colonized protoclones with growth rates less 0.96 mm/d exhibited slow spawn run and failed to complete the spawn-running process even after 45 days. Results are in agreement with the findings of Singh et al (2007). In our study, all appressed protoclones produced structures like primordia in one or two out of three replicates.…”

supporting

confidence: 93%

Screening of homokaryotic protoclones of <i>Agaricus bisporus</i> (J. Lge) Imbach by colony characters and ISSR markers

2010

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Among ten slow-growing protoclones of Agaricus bisporus (J. Lge) Imbach, all appressed colonies showed slower growth rate and spawn run, and inability to produce fruiting bodies in substrate. Seven of 40 inter-simple sequence repeat (ISSR) primers amplified 78 reproducible fragments, 48.93% were polymorphic, each producing 7 to 16 bands ranging from 0.10 to 2.10 kbp, sufficient to differentiate the protoclones from each other. Appressed protoclones were homoallelic at a number of loci that were heteroallelic in the parent, suggesting that they represented rare homokaryons. Thus, using morphological characters along with ISSR, polymorphisms could be useful for quick, easy, and accurate in distinguishing homo-and heterokaryotic isolates.

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“…[8][9][10][11] Breeding studies, and even protoplast fusion techniques, have been used to develop interspecies fusants of A. bitorquis and A. bisporus, searching for strains with high yields and resistance to diseases and fungicides. [12][13][14] Both mushroom species require almost the same cultivation practices, although A. bitorquis prefers higher temperatures and CO 2 levels. A. bitorquis is grown in a mesophilic temperature range of 20 to 30 °C, making it a very important mushroom especially for tropical countries.…”

Section: Introductionmentioning

confidence: 99%

“…Despite slight phylogenetic differences, the biology of Agaricus bitorquis is little different from that of Agaricus bisporus (Lange) Imbach . Breeding studies, and even protoplast fusion techniques, have been used to develop interspecies fusants of A. bitorquis and A. bisporus , searching for strains with high yields and resistance to diseases and fungicides . Both mushroom species require almost the same cultivation practices, although A. bitorquis prefers higher temperatures and CO 2 levels.…”

Section: Introductionmentioning

confidence: 99%

Cultivation of Agaricus bitorquis mushroom as an strategy for the Integrated Pest Management of the myceliophagous mite Microdispus lambi

Navarro¹,

López‐Serrano

Escudero-Colomar

et al. 2020

Pest Management Science

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BACKGROUNDThe phorid fly Megaselia halterata Winnertz (Diptera: Phoridae) is the principal vector of Microdispus lambi (Acari: Pygmephoroidea) in Spanish Agaricus bisporus Lange (Imbach) mushroom farms. This myceliophagous mite does not appear to be a pest in Agaricus bitorquis (Quél.) Sacc mushroom crops. This study explores the role of phorid flies as vectors of Microdispus lambi in Agaricus bitorquis mushroom crops.RESULTSThe incidence of M. lambi in A. bitorquis growing substrates did not reach appreciable levels at any point during the growing cycle. The presence of phorid flies in A. bitorquis farms was normally higher than that in the case of Agaricus bisporus Lange (Imbach) species. The percentage of phorid vectors did not statistically differ between both Agaricus crops during infection periods. However, by the end of the crop, this percentage had increased only in A. bisporus crops, coinciding with a high incidence of mites in the substrate of this mushroom species; Megaselia halterata emerging from the mushroom substrate of A. bitorquis summer crops did not carry mites as they were absent from compost and casing.CONCLUSIONM. halterata is a pest in Spanish A. bitorquis mushroom crops, meanwhile M. lambi, its phorectic mite, has shown not to be a pest of this species mushroom farms during the spring–summer growing season. A. bitorquis crops could potentially be used as an IPM measure to decrease the incidence and prevent the propagation of the myceliophagous mite M. lambi in A. bisporus mushroom growing farms. © 2020 Society of Chemical Industry

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Formation of interspecies fusants of Agaricus bisporus and Agaricus bitorquis mushroom by protoplast fusion

Cited by 8 publications

References 10 publications

Efficiency of RAPD and ISSR Markers in Differentiation of Homo- and Heterokaryotic Protoclones of Agaricus bisporus

Efficiency of RAPD and ISSR Markers in Differentiation of Homo- and Heterokaryotic Protoclones of Agaricus bisporus

Screening of homokaryotic protoclones of <i>Agaricus bisporus</i> (J. Lge) Imbach by colony characters and ISSR markers

Cultivation of Agaricus bitorquis mushroom as an strategy for the Integrated Pest Management of the myceliophagous mite Microdispus lambi

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