The study was conducted at the farmers field in Sylhet under AEZ 20 during three consecutive years 2012-13, 2013-14 and 2014-15 to determine the productivity and profitability of cropping patterns viz., IP: improved pattern (Mung bean-T. aus-T. aman rice) and FP: farmer's pattern (Fallow-T. aus-T. aman rice) through incorporation of high yielding varieties and improved management practices. The experiment was laid out in randomized complete block design with six dispersed replications. Results showed that the improve pattern with management practices provided 10.85 and 14.32% higher grain yield of T. aus and T. aman rice, respectively; also contributed more T. aman mean rice equivalent yield (11.81 t ha -1 ) compared to farmer's pattern. Mean sustainable yield index (77.63%), production efficiency (47.88 kg ha -1 day -1 ), and land use efficiency (67.66%) were maximum in Mung bean-T. aus-T. aman rice cropping system. Similarly, the highest mean gross margin (Tk.126762 ha -1 ) with benefit cost ratio (2.10) was obtained from improved pattern. Three years results revealed that 42% extra cost provides an ample scope of considerable improvement of the productivity of improved pattern with the inclusion of Mungbean before T. aus rice.
An on-farm trial with four orange fleshed sweet potato varieties viz. BARI Mishti Alu-4, BARI Mishti Alu-8, BARI Mishti Alu-12, BARI Mishti Alu-13 including local check (Muli Alu) were evaluated at farming system research and development (FSRD) site, Jalalpur, Sylhet, Bangladesh during rabi season for two consecutive years (2016-2017 & 2017-2018) under Surma‐Kushiyara Floodplain of Bangladesh. The experiment was laid out in a randomized complete block design (RCBD) with six dispersed replications. The results revealed that the genotypes varied considerably in tuber root yields and yield attributing characters. Considering the average tuber root yield performance orange fleshed sweet potato varieties performed better than local variety (Muli Alu). However, among the varieties under studied BARI Mishti Alu-12 produced higher roots yield (40.63 t ha-1) than the other tested varieties. Considering economic return, the highest gross margin (Tk. 5,65,110 ha-1) and benefit cost ratio (5.50:1.00) was also obtained from sweet potato var. BARI Mishti Alu-12 and the lowest returnfrom var. BARI Mishti Alu-4 with benefit cost ratio of 3.54. Though the local cultivar (Muli Alu) produced lower root yields but it provided considerable gross margin (Tk.3,76,240 )and BCR (3.99:1.00 ) which closed to BARI Mishti Alu-12. It is due to higher demand of local cultivar with high price in the local market. Therefore, the genotypes BARI Mishti Alu-12 and local cultivar (Muli Alu) were found suitable for their better growth and yield under the acidic soils
Bangladesh Agron. J. 2018, 21(2): 59-65
Morphologically different fifteen slow growing single spore isolates (SSIs) were screened from germinated basidiospores of Agaricus bisporus; assumed to be homokaryons, and subjected to growth rate, spawn run, fruiting and inter-simple sequence repeat (ISSR) analysis for confirmation of true homokaryons. The present studies are the first report of fingerprinting on differentiating homo- and hetero-karyotic SSIs using ISSR markers. The patterns were highly polymorphic and very reproducible. Among 40 primers tested, 7 ISSR primers were selected for the analysis of genomic DNA and generated a total of 54 ISSR fragments, sufficient to differentiate the 15 isolates from each other. ISSR fingerprinting detected 46.30% polymorphic loci. All appressed homokaryons carried a subset of ISSR markers found in the heterokaryons, and these were not able to produce a fruiting body. A test of cross-fertility and the following fruiting trial proved that 3 of the 15 SSIs with different ISSR fingerprints were homokaryons. None of the discarded SSIs have any missing bands present in the parental heterokaryotic control; these are heterokaryons derived from non-sister nuclear pairs. It is revealed that homokaryons are probably restricted to the assumed morphological classes of SSIs. These results demonstrate that ISSR markers provide an efficient alternate for identification of homokaryons and suggest these markers be considered as new tools for the survey of Agaricus species.
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