Screening of homokaryotic protoclones of <i>Agaricus bisporus</i> (J. Lge) Imbach by colony characters and ISSR markers

Abstract

Among ten slow-growing protoclones of Agaricus bisporus (J. Lge) Imbach, all appressed colonies showed slower growth rate and spawn run, and inability to produce fruiting bodies in substrate. Seven of 40 inter-simple sequence repeat (ISSR) primers amplified 78 reproducible fragments, 48.93% were polymorphic, each producing 7 to 16 bands ranging from 0.10 to 2.10 kbp, sufficient to differentiate the protoclones from each other. Appressed protoclones were homoallelic at a number of loci that were heteroallelic in the parent, suggesting that they represented rare homokaryons. Thus, using morphological characters along with ISSR, polymorphisms could be useful for quick, easy, and accurate in distinguishing homo-and heterokaryotic isolates.

Distinguishing Homokaryons and Heterokaryons in Medicinal Polypore Mushroom Wolfiporia cocos (Agaricomycetes) Based on Cultural and Genetic Characteristics

“…In Agaricus bisporus , the traditional identification of homokaryotic strains is generally dependent on their morphological characteristics and isozyme differences ( Li and Fang, 2002 ). The SSIs identified as homokaryons exhibited an inability to produce fruiting bodies and a slower and commonly less vigorous spawn run process, and they grew more slowly than heterokaryons in A. bisporus ( Nazrul et al, 2010 ). Homokaryons grew more slowly, appearing more uniform in culture than heterokaryons, and could not form basidiocarps in culture in Phellinus weirii ( Hansen, 1979 ).…”