Forensic parameters of the Investigator DIPplex kit (Qiagen) in six Mexican populations

Abstract

Allele frequencies and statistical parameters of forensic efficiency for 30 deletion-insertion polymorphisms (DIPs) were estimated in six Mexican populations. For this purpose, 421 unrelated individuals were analyzed with the Investigator DIPplex kit. The Hardy-Weinberg and linkage equilibrium was demonstrated for this 30-plex system in all six populations. We estimated the combined power of discrimination (PD ≥ 99.999999%) and combined power of exclusion (PE ≥ 98.632705%) for this genetic system. A low but significant genetic structure was demonstrated among these six populations by pairwise comparisons and AMOVA (F ST ≥ 0.7054; p ≤ 0.0007), which allows clustering populations in agreement with geographical criteria: Northwest, Center, and Southeast.

Population genetic structure analysis and forensic evaluation of Xinjiang Uigur ethnic group on genomic deletion and insertion polymorphisms

“…InDel data from 21 previously published groups including 9 Chinese groups: Beijing Han, Tibetan, Kazak, Urumchi Uigur (represented previously reported samples from Urumchi of Xinjiang) (Wei et al 2014 ), Guangdong Han (Hong et al 2013 ), Shanghai Han, She (Wang et al 2014 ), Yi (Zhang et al 2015 ) and Xibe (Meng et al 2015 ); 6 Mexican groups: Chihuahua Mexican, Mexico Mexica, Jalisco Mexican, Veracruz Mexican, Yucatan Mexican and Mexican Amerindian (Martínez-Cortés et al 2015 ); South Korean (Korea) (Seong et al 2014 ); Dane (Denmark) (Friis et al 2012 ); Two Spanish groups: Basque and Central Spanish (Martín et al 2013 ); Uruguayan (Uruguay) (Saiz et al 2014 ); and Hungarian (Hungary) (Kis et al 2012 ) were collected for population genetic analysis.…”

Autosomal InDel polymorphisms for population genetic structure and differentiation analysis of Chinese Kazak ethnic group

“…Interpopulation differentiations were compared using the analyses of molecular variance (AMOVA) method. As shown in Supplementary Table 2 , we calculated pairwise p values between the studied Kazak group and 23 previously published populations including Kazak1 [ 7 ], Uigur [ 7 ], Yi [ 10 ], Xibe [ 11 ], Tujia [ 12 ], South Korean [ 13 ], She [ 14 ], two Tibetan groups [ 15 ], three Han populations [ 7 , 14 , 16 ] in different regions, six Mexican groups [ 17 ], four European groups and Uruguayan group [ 18 – 21 ] (The geographical locations of the studied Kazak and other reference populations were shown in Supplementary Figure 2 ) based on allele frequencies of 30 InDel loci. The least differences were observed between the studied Kazak group and Kazak1, Uigur groups with significant differences at 1, 6 loci, respectively.…”

Population Genetics and Forensic Efficiency of 30 InDel Markers in Four Chinese Ethnic Groups Residing in Sichuan

“…To comprehensively explore the patterns of genetic affinity between our studied populations and worldwide reference populations, we merged our newly obtained population data with those of 61 worldwide populations. The final database used in the population comparison study included 6483 East Asian individuals from 38 populations [4,5,[8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26], 494 South Asians from three populations [27][28][29], 300 Vietnamese of Southeast Asia [30], 194 West Asians from two populations [31], 621 African individuals from five populations [27,30,32], 984 European individuals from eight populations [4,[31][32][33][34][35] and 1 071 South Americans from eight populations [36,38]. The detailed geographical regions and population sizes of these included populations are submitted in Supplementary Table S1.…”