Population Genetics and Forensic Efficiency of 30 InDel Markers in Four Chinese Ethnic Groups Residing in Sichuan

Wang, Fei; He, Guanglin; Wang, Zheng; Wang, Mengge; Liu, Jing; Zou, Xing; Wang, Shouyu; Song, Mengyuan; Ye, Ziwei; Xie, Mingkun; Hou, Yiping

doi:10.1080/20961790.2020.1737470

Cited by 2 publications

(2 citation statements)

References 41 publications

(45 reference statements)

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“…To maximize the human identification effectiveness of InDel in Chinese population, novel InDels with higher heterozygosity levels should be identified to improve the marker set. In the present study, we select 34 autosomal InDel loci with the minimum allele frequency (MAF) ≥ 0.25 in East Asia, which show no overlap with the InDel markers from the Investigator ® DIPplex kit [ 34 , 35 ]. Based on the six-dye fluorescence labeling technology, a multiplex InDel kit containing all the above InDels is constructed and commercialized for Chinese population [ 36 ].…”

Section: Introductionmentioning

confidence: 99%

Developmental Validation of the Novel Six-Dye GoldeneyeTM DNA ID System 35InDel Kit for Forensic Application

Yang

et al. 2021

Forensic Sciences Research

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Insertion/deletion polymorphisms (InDels) have been treated as a prospective and helpful genetic marker in the fields of forensic human identification, anthropology and population genetics for the past few years. In this study, we developed a six-dye multiplex typing system consisting of 34 autosomal InDels and Amelogenin for forensic application. The contained InDels were specifically selected for Chinese population with the MAF ≥ 0.25 in East Asia, which do not overlap with the markers of Investigator ® DIPplex kit. The typing system was named as Goldeneye TM DNA ID System 35InDel Kit, and a series of developmental validation studies including repeatability/reproducibility, concordance, accuracy, sensitivity, stability, species specificity and population genetics were conducted on this kit. We confirmed that the 35InDel kit is precise, sensitive, species specific and robust for forensic practice. Moreover, the 35InDel kit is capable of typing DNA extracted from forensic routine case-type samples as well as degraded samples and mixture samples. All markers are proved to be highly polymorphic with an average observed heterozygosity (He) of 0.4582. The combined power of discrimination (CPD) is 0.999 999 999 999 978 and the combined power of exclusion in duos (CPE D ) and trios (CPE T ) are 0.978 837 and 0.999573, respectively, which are higher than those of the Investigator® DIPplex kit. Thus, the Goldeneye TM DNA ID System 35InDel kit is suitable for forensic human identification and could serve as a supplementary typing system for paternity testing.

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Section: Introductionmentioning

confidence: 99%

Developmental Validation of the Novel Six-Dye GoldeneyeTM DNA ID System 35InDel Kit for Forensic Application

Yang

et al. 2021

Forensic Sciences Research

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“…As the biallelic DNA length polymorphism in human genome, InDels possess highlighted merits such as wide distributions in human genome, low mutation rates, small amplified fragments, absence of stutter peaks in comparisons with STRs and available for capillary electrophoresis (CE) platform [ 5 , 6 ]. InDels complement the disadvantages of STRs, which have become the promising genetic markers in the applications of HID [ 7 , 8 ] and biogeographic ancestry predictions [ 9 ] since the initial InDel map was reported in 2006 [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

An Interpretation of the Genetic Polymorphism and Population Genetic Background of Ankang Han Population via a Novel InDel Panel

Cui

Jin

Fang

et al. 2021

Forensic Sciences Research

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In this research, genotyping data of 43 InDel loci in 311 Han individuals in Ankang City, Shaanxi Province, China were detected using a self-developed five-dye multiplex amplification panel. The allelic frequencies and forensic parameters of all InDel loci were calculated. The combined power of discrimination and probability of exclusion values were 0.999 999 999 999 999 998 827 39 and 0.999 887 424, respectively, which demonstrated that this 43-InDel panel was powerful for individual identifications in Ankang Han population. Moreover, genetic distances, pairwise F ST values, principal component analyses, phylogenetic trees and STRUCTURE analyses were performed to investigate the genetic affinities between Ankang Han and reference groups. Population genetic investigations indicated that Ankang Han population had a close genetic relationship with Southern Han population compared with other reference groups.

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Population Genetics and Forensic Efficiency of 30 InDel Markers in Four Chinese Ethnic Groups Residing in Sichuan

Cited by 2 publications

References 41 publications

Developmental Validation of the Novel Six-Dye GoldeneyeTM DNA ID System 35InDel Kit for Forensic Application

Developmental Validation of the Novel Six-Dye GoldeneyeTM DNA ID System 35InDel Kit for Forensic Application

An Interpretation of the Genetic Polymorphism and Population Genetic Background of Ankang Han Population via a Novel InDel Panel

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