Forensic evaluation of the QIAshredder/QIAamp DNA extraction procedure

Castella, Vincent; Dimo-Simonin, N.; Brandt-Casadevall, C.; Mangin, Patrice

doi:10.1016/j.forsciint.2005.11.012

Cited by 59 publications

(28 citation statements)

References 12 publications

(19 reference statements)

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“…Direct binding of agents to single-stranded or double-stranded DNA can prevent amplification" [31]. Each nucleated cell contains two copies of an individual's genome and can be used to generate a DNA profile under optimal conditions [32]. DNA can be isolated substantially from any part of human body.…”

Section: Resultsmentioning

confidence: 99%

Short Tandem Repeat Typing Using Endogenous DNA Isolated from Human Fingernail

Shehab¹

2018

JNUS

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Genomic DNA was isolated from variable biological sources. Nails contain genomic DNA but there is limitation in isolating of genetic material from this tissue due to the special composition and structure of this tissue in which DNA embedded in keratinized cells. This study aimed to investigate the capability of using DNA that isolated from fingernails in forensic analysis. Thirty fingernails clipping were collected from three groups (10 fingernail samples for children, 10 fingernail samples for adolescent and 10 fingernail samples for adult) in addition to thirty buccal swabs collected from the same volunteers as reference samples. Both fingernail clipping and buccal swabs were subjected to DNA extraction using phenol/chloroform extraction. All samples were typed for fifteen autosomal STR loci along with amelogenin, using the Applied Biosystems AmpFiSTR® Identifiler™ kit. Our finding showed that input of 5 mg nail material (1 to 2 of fingernail clippings) gives a variable yield of DNA concentration also there was no significant difference in The mean of concentration for samples that isolated from whole fingernail and those isolated from fingernail after cut into small pieces for children groups while there were significant difference for adult and adolescent groups (0.107702, 0.07544 and 0.000192 respectively).

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Section: Resultsmentioning

confidence: 99%

Short Tandem Repeat Typing Using Endogenous DNA Isolated from Human Fingernail

Shehab¹

2018

JNUS

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“…for two separate DNA extractions, one of which was realized with a standard phenol/chloroform protocol. All other DNA extractions were performed with the QIAshredder/ QIAamp method [3] using single swabs, pooled double swabs or small objects. DNA extracts were concentrated with Microcon 30 centrifugal filters (Millipore AG) in order to get 25 ml final volume.…”

Section: Methodsmentioning

confidence: 99%

DNA profiling success and relevance of 1739 contact stains from caseworks

Castella

Mangin

2008

Forensic Science International: Genetics Supplement Series

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“…Silica-based extraction methods have become widely available as part of a commercial kit, making them more costly than either phenol or Chelex ® , but they have the advantage of being open to automation. The silica-based material 10 is embedded in a layer at the base of a spin column, through which fluid containing DNA can pass. The DNA binds to the silica membrane, but all the molecules that do not possess a strong negative charge pass through the membrane and are discarded.…”

Section: +mentioning

confidence: 99%

Forensic DNA profiling: state of the art

Linacre¹,

Templeton²

2014

RRFMS

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DNA profiling is now a routine test, yet it has only been 26 years since its first use. During these intervening years, the science of DNA profiling has undergone many changes, and it will continue to do so in the future. DNA profiling in the laboratory starts with DNA extractions and is followed by quantification of the DNA, amplification of up to 23 human specific short tandem repeat loci, and separation on capillary columns. The final stage is interpretation of the data leading to reporting the DNA evidence to a court. Each of these steps has seen improvements leading to higher sensitivity from minute trace samples. Genetic markers on the X and Y chromosomes are now used for both criminal and civil investigations, along with mitochondrial DNA. Mitochondrial DNA has been used in forensic science since the 1990s because of its high copy number and particular maternal inheritance. These sex chromosome markers and mitochondrial DNA were used effectively to determine the identity or ancestry of individuals, and now other genetic markers can be used to determine certain phenotypic traits to a high degree of accuracy. Genetic testing adapted from medical and pharmaceutical sciences, such as next-generation DNA sequencing, will soon be applied to mainstream forensic science, opening new avenues in criminal investigations. This review aims to cover these key events and advancements in the field from both a historical view and current practice. Keywords: forensic science, DNA profiling, short tandem repeats, mitochondrial DNA, phenotypic testing, next-generation sequencing Opening commentsDNA profiling has revolutionized the process of forensic human identification. It is of note that this is a relatively recent addition to the forensic science tool kit and that it is constantly undergoing developments. The aim of this review is to take the reader through the processes of DNA typing, starting with DNA isolation from biological material through novel approaches and phenotypic testing. The review is written for those in forensic medicine; therefore, certain knowledge is assumed, but equally, aspects that are specific to forensic science will be emphasized and placed into the right context. For those who wish more information than can be provided within the scope of this review, there are books ranging from introductory texts 1 to those that are more comprehensive.2,3

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Forensic evaluation of the QIAshredder/QIAamp DNA extraction procedure

Cited by 59 publications

References 12 publications

Short Tandem Repeat Typing Using Endogenous DNA Isolated from Human Fingernail

Short Tandem Repeat Typing Using Endogenous DNA Isolated from Human Fingernail

DNA profiling success and relevance of 1739 contact stains from caseworks

Forensic DNA profiling: state of the art

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