DNA profiling success and relevance of 1739 contact stains from caseworks

Avoiding Errors and Pitfalls in Evidence Sampling for Forensic Genetics

“…The second step is to complete the swabbing by the application of dry swabs to recapture the moisture containing hydrated cells. Co-extraction of these swabs to enhance overall retrieval of DNA is recommended (Castella and Mangin, 2008;Pang and Cheung, 2007). The moistening agent can be sterile water, 0, 01% sodium dodecyl sulphate (Wickenheiser, 2002) or isopropanol (Hansson et al, 2009).…”

Evaluation of Low Trace DNA Recovery Techniques from Ridged Surfaces

“…The swabs are then combined prior to the extraction phase. This technique is generally used on non-absorbent surfaces [3,4].…”

“…This technique is generally used on absorbent surfaces, such as clothing, as the wet swab can cause a 'wicking' effect in which the DNA material is drawn down through the fabric away from the surface; therefore making it difficult recover the DNA material. Although the majority of ridged surfaces are not considered to be absorbent, this technique is included for completeness [3,4].…”

Knowledge on DNA Success Rates to Optimize the DNA Analysis Process: From Crime Scene to Laboratory

“…Different studies have given some insight into DNA success rates . For example, in a previous study, blood stains and saliva traces from cigarette ends show a high success rate in providing the CJS with DNA typing results .…”

“…To reduce this uncertainty, a thorough analysis on DNA success rates is the necessary first step toward creating a decision support system. Different studies have given some insight into DNA success rates (9)(10)(11)14). For example, in a previous study, blood stains and saliva traces from cigarette ends show a high success rate in providing the CJS with DNA typing results (9).…”