Food- and gender-dependent pharmacokinetics of paeoniflorin after oral administration with Samul-tang in rats

Hwang, Youn–Hwan; Kim, Taesoo; Cho, Won‐Kyung; Jang, Doorye; Ha, Jung-Ho; Yeul, Jin

doi:10.1016/j.jep.2012.04.032

Cited by 18 publications

(17 citation statements)

References 41 publications

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“…That is possibly due to the significantly higher expression level of UGT2B1 in female rat livers than that in male ( p < 0.001; Takeuchi et al, ). However paeoniflorin showed gender‐dependent PK profiles, different from the published report (Hwang et al, ); AUC, t 1/2 and peak concentration values of female rats were greater than those of male rats, which may be caused by drug interactions from the different formula or/and the different dosages administered. In the literature, the rats were given a single oral dose of Samul‐tang (composed of four herbal medicines) equivalent to 80 mg/kg of paeoniflorin (Hwang et al, ), while in this study, 11.68 mg/kg of paeoniflorin from HGKYC (composed of 12 herbal medicines) was administered orally to rats.…”

Section: Resultscontrasting

confidence: 87%

“…However paeoniflorin showed gender‐dependent PK profiles, different from the published report (Hwang et al, ); AUC, t 1/2 and peak concentration values of female rats were greater than those of male rats, which may be caused by drug interactions from the different formula or/and the different dosages administered. In the literature, the rats were given a single oral dose of Samul‐tang (composed of four herbal medicines) equivalent to 80 mg/kg of paeoniflorin (Hwang et al, ), while in this study, 11.68 mg/kg of paeoniflorin from HGKYC (composed of 12 herbal medicines) was administered orally to rats. Schisandrin in male rats could not be detected under the experimental conditions, while in female rats it showed a classic PK profile of oral administration (showed in Figure ), which was mainly caused by the differences in metabolism between male and female rats.…”

Section: Resultscontrasting

confidence: 87%

“…A simple and rapid method for the simultaneous determination of the five compounds by HPLC‐MS/MS using positive–negative scanning ion mode was developed and validated on the basis of US Food and Drug Administration bioanalytical method validation guidance (US Department of Health and Human Services Food and Drug Administration, ). It was successfully used to study the pharmacokinetics of the five compounds in rats, and the results indicated that the herb–herb interactions affected the PK profiles of the five compounds compared with the available references (Fong et al, ; Hwang et al, ; Tong et al, ; Xu et al, ), which needs to be further studied in future work.…”

Section: Discussionmentioning

confidence: 99%

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Simultaneous determination of wogonin, oroxylin a, schisandrin, paeoniflorin and emodin in rat serum by HPLC–MS/MS and application to pharmacokinetic studies

Chen

Wei

Luo

et al. 2017

Biomedical Chromatography

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Wogonin and oroxylin A in Scutellariae Radix, schisandrin in Chinensis Fructus, paeoniflorin in Moutan Cortex and emodin in Polygoni Cuspidate Rhizome et Radix are anti-inflammatory active compounds. A method for simultaneous determination of the five compounds in rat was developed and validated using high-performance liquid chromatography with tandem mass spectrometry (HPLC-MS/MS). The separation was performed on a Symmetry C column (4.6 × 50 mm, 3.5 μm) with acetonitrile and 0.1% formic acid aqueous solution as the mobile phases. The detection was performed using multiple-reaction monitoring with electrospray ionization source in positive-negative ion mode. The calibration curves showed good linearity (r ≥ 0.9955). The lower limit of quantification (LLOQ) was 5 ng/mL for wogonin and schisandrin, 10 ng/mL for oroxylin A and emodin, and 15 ng/mL for paeoniflorin, respectively. The relative standard deviations of intraday and interday precisions were <11.49 and 14.28%, respectively. The extraction recoveries and matrix effects were acceptable. The analytes were stable under the experiment conditions. The validated method has been successfully applied to pharmacokinetic studies of the five compounds in rats after oral administration of Hu-gan-kan-kang-yuan capsule. This paper would be a valuable reference for pharmacokinetic studies of Chinese medicine preparations containing the five compounds.

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Section: Resultscontrasting

confidence: 87%

Section: Resultscontrasting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

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Simultaneous determination of wogonin, oroxylin a, schisandrin, paeoniflorin and emodin in rat serum by HPLC–MS/MS and application to pharmacokinetic studies

Chen

Wei

Luo

et al. 2017

Biomedical Chromatography

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“…Samul-tang was prepared in accordance with the method previously described. [17] Brownish powder (403.5 ± 5.4 g) of Samul-tang was obtained and stored at 4°C before use. The amounts of nodakenin, nodakenetin, decursinol, and decursin/decursinol angelate in Samul-tang were 2.29 ± 0.14, 0.08 ± 0.01, 0.094 ± 0.01, and 5.24 ± 0.12 mg/g extract, respectively, according to previous report.…”

Section: Methodsmentioning

confidence: 99%

High-performance liquid chromatography determination and pharmacokinetics of coumarin compounds after oral administration of Samul-Tang to rats

et al. 2014

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Background:Samul-tang has been traditionally used for the treatment of cardiovascular, gynecologic, cutaneous, and chronic inflammation disorders. Although coumarin compounds do have various pharmacological activities and the same may be present in Samul-tang, however there is little information about it.Objective:A simple and sensitive high-performance liquid chromatography (HPLC) method has been developed for the determination of nodakenin, nodakenetin, decursin, decursinol, and decursinol angelate in rat plasma. To obtain a better understanding for pharmacological properties of Samul-tang, pharmacokinetic study of coumarin compounds was performed after oral administration of Samul-tang in rats.Materials and Methods:Chromatographic separation of the analytes was successfully achieved on a Phenomenex Luna C18 column (4.6 mm×250 mm, 5 μm) using a mobile phase composed of acetonitrile water with a gradient elution at a flow rate of 1 mL/min. Noncompartmental analysis was performed.Results:Calibration curves for all analytes had good linearity (r2 <0.999) in a wide linear range. The lower limit of quantification (LLOQ) ranged from 0.05 to 0.1 μg/mL. The variation of intra- and interday assay was less than 15%. Nodakenin, nodakenetin, and decursinol were determined in rat plasma after oral administration of Samul-tang.Conclusion:This developed and validated HPLC method was successfully applied to the pharmacokinetic study of three coumarin compounds in rats, given as a single oral administration of Samul-tang. These pharmacokinetic data of the nodakenin, nodakenetin, and decursinol could offer a new point of view to evaluate the pharmacological effects of Samul-tang.

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“…Using PKSolver program, the noncompartmental pharmacokinetic analysis was conducted in accordance with Hwang et al [14]. The absolute bioavailability of ciprofloxacin ( F ) after berberine- and HR-treatment was determined as F = (AUC p.o.…”

Section: Methodsmentioning

confidence: 99%

Effects of Berberine and Hwangryunhaedok-Tang on Oral Bioavailability and Pharmacokinetics of Ciprofloxacin in Rats

Hwang

Cho

Jang

et al. 2012

Evidence-Based Complementary and Alternative Medicine

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Hwangryunhaedok-Tang (HR) and berberine-containing single herbs are used to treat bacterial infection and inflammatory diseases in eastern Asia. The combination of berberine-containing herbal medicines and ciprofloxacin can be an excellent antibacterial chemotherapy against multidrug resistance bacteria. To evaluate the pretreatment effect of berberine and HR, vehicle, berberine (25 and 50 mg/kg/day), and HR (1.4 g/kg/day) were daily administered to rats for five consecutive days. On day 6, ciprofloxacin was administered (10 mg/kg, i.v. and 20 mg/kg, p.o.) to rats. To assess cotreatment effect of berberine and ciprofloxacin, berberine (50 mg/kg) and ciprofloxacin (20 mg/kg) were coadministered by single oral gavage. Pharmacokinetic data were estimated by noncompartmental model. Compared with ciprofloxacin alone (control group), coadministration of berberine (50 mg/kg) and ciprofloxacin significantly decreased C max of ciprofloxacin (P < 0.05). In addition, the pretreatment of berberine (50 mg/kg/day) and HR (1.4 g/kg/day) significantly decreased C max and AUC0→∞, compared with control group (P < 0.05). The oral bioavailability of ciprofloxacin was reduced by cotreatment of berberine and pretreatment of berberine and HR. Our results suggest that the expression of P-glycoprotein and organic anion and/or organic cation transporters (OAT/OCT) could take a role in reduced oral bioavailability of ciprofloxacin by berberine and HR.

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Food- and gender-dependent pharmacokinetics of paeoniflorin after oral administration with Samul-tang in rats

Cited by 18 publications

References 41 publications

Simultaneous determination of wogonin, oroxylin a, schisandrin, paeoniflorin and emodin in rat serum by HPLC–MS/MS and application to pharmacokinetic studies

Simultaneous determination of wogonin, oroxylin a, schisandrin, paeoniflorin and emodin in rat serum by HPLC–MS/MS and application to pharmacokinetic studies

High-performance liquid chromatography determination and pharmacokinetics of coumarin compounds after oral administration of Samul-Tang to rats

Effects of Berberine and Hwangryunhaedok-Tang on Oral Bioavailability and Pharmacokinetics of Ciprofloxacin in Rats

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