High-performance liquid chromatography determination and pharmacokinetics of coumarin compounds after oral administration of Samul-Tang to rats

Abstract

Background:Samul-tang has been traditionally used for the treatment of cardiovascular, gynecologic, cutaneous, and chronic inflammation disorders. Although coumarin compounds do have various pharmacological activities and the same may be present in Samul-tang, however there is little information about it.Objective:A simple and sensitive high-performance liquid chromatography (HPLC) method has been developed for the determination of nodakenin, nodakenetin, decursin, decursinol, and decursinol angelate in rat plasma. To obtain a better understanding for pharmacological properties of Samul-tang, pharmacokinetic study of coumarin compounds was performed after oral administration of Samul-tang in rats.Materials and Methods:Chromatographic separation of the analytes was successfully achieved on a Phenomenex Luna C18 column (4.6 mm×250 mm, 5 μm) using a mobile phase composed of acetonitrile water with a gradient elution at a flow rate of 1 mL/min. Noncompartmental analysis was performed.Results:Calibration curves for all analytes had good linearity (r2 <0.999) in a wide linear range. The lower limit of quantification (LLOQ) ranged from 0.05 to 0.1 μg/mL. The variation of intra- and interday assay was less than 15%. Nodakenin, nodakenetin, and decursinol were determined in rat plasma after oral administration of Samul-tang.Conclusion:This developed and validated HPLC method was successfully applied to the pharmacokinetic study of three coumarin compounds in rats, given as a single oral administration of Samul-tang. These pharmacokinetic data of the nodakenin, nodakenetin, and decursinol could offer a new point of view to evaluate the pharmacological effects of Samul-tang.

Coumarins: The Antimicrobial agents

“…33 Al-Amiery et al have synthesized 4-[(5-mercapto-4-phenyl-4H-1,2,4triazol-3-yl)-methoxy]-2H-chromen-2-one as coumarin derivatives and their antifungal activity was determined based on the growth inhibition rates of the mycelia of strains of Aspergillus niger and Candida albicans in Potato Dextrose Broth medium (PDB) against concentrations ranging from 10 to 100µgml -1 . Two compounds (5,6) showed good activity as antifungals against fluconazole standard drug. 34 Behrami et al synthesized 8-amino-4,7-dihydroxy-chromen-2-one coumarin derivatives.…”

“…There are a number of reports that natural and synthetic Coumarin derivatives posses antimicrobial activity. [1][2][3][4][5][6][7][8][9][10][11][12][13][14] Novobiocin and Chlorobiocin are established antimicrobials containing a Coumarin (i.e.2H-1-benzopyran-2-ones) skeleton, there are many Coumarin derivatives which have been reported for anticoagulant, anti-inflammatory, anti-HIV, antioxidant, anti-allergic, anti-cancer, anti proliferative and antiviral activities. [15][16][17] It was found that when one biodynamic heterocyclic system was coupled with another heterocyclic system, enhanced biological activity was produced.…”

Simultaneous Determination of Decursin, Decursinol Angelate, Nodakenin, and Decursinol of Angelica gigas Nakai in Human Plasma by UHPLC-MS/MS: Application to Pharmacokinetic Study

“…Despite these pharmacological activities, pharmacokinetic (PK) studies for major components of AGN are limited. Several PK studies of AGN in mice [ 13 ], rats [ 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 ], and humans [ 23 ] have been published. Most of them have characterized PKs of one or two components of courmarins from extracted AGN or shown PKs of combined D and DA.…”

“…Most of them have characterized PKs of one or two components of courmarins from extracted AGN or shown PKs of combined D and DA. One publication has reported PKs for four coumarins of D, DA, nodakenin, and DOH [ 14 ]. However, only combined D and DA could be evaluated in that study because their analytical methods could not separate isomeric peaks of D and DA.…”

“…During the past few decades, many analytical methods using various quantitative analysis instruments—including HPLC-UV [ 13 , 14 , 15 , 16 , 17 , 20 ], LC-MS/MS [ 18 , 19 ], and UHPLC-MS/MS [ 23 ]—have been reported for coumarins from AGN root. Most of these publications have suggested analytical methods for one or two components of coumarins in rat plasma.…”