Simultaneous determination of wogonin, oroxylin a, schisandrin, paeoniflorin and emodin in rat serum by HPLC–MS/MS and application to pharmacokinetic studies

Abstract

Wogonin and oroxylin A in Scutellariae Radix, schisandrin in Chinensis Fructus, paeoniflorin in Moutan Cortex and emodin in Polygoni Cuspidate Rhizome et Radix are anti-inflammatory active compounds. A method for simultaneous determination of the five compounds in rat was developed and validated using high-performance liquid chromatography with tandem mass spectrometry (HPLC-MS/MS). The separation was performed on a Symmetry C column (4.6 × 50 mm, 3.5 μm) with acetonitrile and 0.1% formic acid aqueous solution as the mobile phases. The detection was performed using multiple-reaction monitoring with electrospray ionization source in positive-negative ion mode. The calibration curves showed good linearity (r ≥ 0.9955). The lower limit of quantification (LLOQ) was 5 ng/mL for wogonin and schisandrin, 10 ng/mL for oroxylin A and emodin, and 15 ng/mL for paeoniflorin, respectively. The relative standard deviations of intraday and interday precisions were <11.49 and 14.28%, respectively. The extraction recoveries and matrix effects were acceptable. The analytes were stable under the experiment conditions. The validated method has been successfully applied to pharmacokinetic studies of the five compounds in rats after oral administration of Hu-gan-kan-kang-yuan capsule. This paper would be a valuable reference for pharmacokinetic studies of Chinese medicine preparations containing the five compounds.

Determination of Schisandrin A and Schisandrin B in Traditional Chinese Medicine Preparation Huganpian Tablet by RP-HPLC

“…UV Detection Wavelength for Schisandrin A and Schisandrin B The UV absorption of Schisandrin A and Schisandrin B is reported in a wide range between 210 to 250 nm. 8,16) In this work, wavelengths between 210-250 nm were compared for UV detection of Schisandrin A and Schisandrin B. The results showed that the detection sensitivity decreased with the wavelength increasing; 210 nm gave highest detection sensitivity.…”

“…Chromatographic methods are most commonly used for qualitative identification or quantitation of those natural dibenzocyclooctadiene lignans in raw herbal materials, and medicine or biological matrix samples. [6][7][8][9][10][11][12][13][14][15][16][17][18][19] In this work, a HPLC method was developed for separation and quantitation of Schisandrin A and Schisandrin B, with methanol-water as mobile phase, and UV detection. The present method was used to determine Schisandrin A and Schisandrin B in Huganpian Tablets sample.…”

Pharmacokinetics of B-Ring Unsubstituted Flavones

“…The PK of baicalin, wogonoside, and oroxylin A glucuronide, showed obvious differences between administration as single herb (Huangqin-Tang) or as a compound prescription, with an increase in C max and AUC 0—lim , particularly for wogonoside [246]. In the PK analysis of a Chinese herbal mixture (Hu-gan-kan-kang-yuan capsule), the half-lives of wogonin and oroxylin A were much longer (around 40 h for both) than those reported in the literature, a difference probably also related to the influences of the other components of the formulation [247]. Co-administration of extracts from Scutellariae herba and Acacia catechu (L.f.) Willd.…”

Simultaneous Determination of Multiple Active Components from Bushen Pills and Application in a Pharmacokinetic Study in Rats

“…Schizandrin (SCH, Figure 1(c)), which exhibited antioxidization, enhancing immunity, and antitumor effects, is a major bioactive lignan in Fructus Schisandrae Chinensis [14,15]. Up to now, some methods based on LC-MS or LC-MS/MS have been previously described to quantify PF, BBR, and SCH in the preparations or biological samples in other TCM formula or single herbs [16][17][18][19][20]. However, there is still no report on determining the three active ingredients simultaneously for content determination or pharmacokinetic investigations.…”