Folate-modified, indocyanine green-loaded lipid-polymer hybrid nanoparticles for targeted delivery of cisplatin

Abstract

Cisplatin is a potent antitumor drug, which is widely applied in clinical cancer treatment. However, cisplatin can hardly distinguish between healthy tissue and tumor tissue, resulting in serious toxic side effects. Indocyanine green (ICG) is a FDA-approved near-infrared (NIR) fluorescence dye which has been used in photothermal therapy and optically mediated diagnostic, but the application of ICG is limited by its concentration-dependent aggregation, poor aqueous stability in vitro, lack of target specificity and rapid elimination from the body. Herein, to overcome these limitations of cisplatin and ICG, we fabricated folate-modified, cisplatin, ICG-loaded lipid-polymer hybrid nanoparticles (FCINPs) using a single-step sonication method. The FCINPs exhibited well-defined monodispersity, significant stability and excellent NIR penetration ability. The intracellular uptake experiment showed that the targeting efficacy of the FCINPs was more effective in folate receptors (FRs) over-expressing MCF-7 cells than FRs negative A549 cells. In addition, compared with chemo or photothermal treatment alone, the treatment of FCINPs in combination with 808 nm NIR laser irradiation can significantly induce the apoptosis and necrosis of MCF-7 cells. These findings indicated that the FCINPs would be a promising nanosized drug formulation for tumor-targeted therapy in the future.

<p>Lipid-polymer hybrid nanoparticles for controlled delivery of hydrophilic and lipophilic doxorubicin for breast cancer therapy</p>

“…Moreover, the polymeric carriers offer prolonged and controlled drug release from the system and excellent stability profiles. Despite all of these properties, both liposomes and polymeric carrier systems have limitations in terms of drug stability, drug leakage, low loading capacity, and compatibility 8…”

<p>Chlorins e6 loaded silica nanoparticles coated with gastric cancer cell membrane for tumor specific photodynamic therapy of gastric cancer</p>

“…The amine SLN was prepared using a water-in-oil microemulsion according to a previousreport 30. In brief, the water-in-oil microemulsion was firstly prepared and stirred properly to obtain transparent solution.…”

<p>Lipid&ndash;polymer hybrid nanoparticles as a next-generation drug delivery platform: state of the art, emerging technologies, and perspectives</p>

“…Moreover, treatment of targeted NPs with 808 nm near infra-red laser irradiation induced substantial cell death by apoptosis and necrosis of MCF-7 cells compared with standard care therapy. 76 Using an ESE technique, a DOX-encapsulated LPHNP was constructed with PLGA-lecithin-DSPE-PEG and further decorated with DSPE-PEG-folate. It had been shown that folate-targeted NPs confer higher uptake and cytotoxicity in MCF-7 cells compared with nontargeted NPs.…”