Fluorometric Determination of Reserpine and Related Compounds by Reaction with Vanadium Pentoxide

Urbányi, Tibor; Stober, Henry

doi:10.1002/jps.2600591226

Cited by 20 publications

(1 citation statement)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Reserpine is converted to 3,4-dehydroreserpine, which exhibits intense green–yellow fluorescence that can be directly detected by a fluorescence detector. Many oxidants, such as nitrous acid [12], cerium (IV) sulfate [16], vanadium pentoxide [24], periodate ion [25] and 2-iodoxybenzoate [26], have been used for reserpine derivatization. Although existing derivatization techniques have their own merits, they are time-consuming and lack sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Fluorimetric detection of reserpine in mouse serum through online post-column electrochemical derivatization

Chen

et al. 2018

R. Soc. open sci.

View full text Add to dashboard Cite

A novel method combining high-performance liquid chromatography with online post-column electrochemical derivatization and fluorescence detection was established for the detection of reserpine in mouse serum. Reserpine separation was conducted using a C18 column with 5 mM H3PO4 and acetonitrile (55/45, v/v) as eluent. Reserpine was then electro-oxidized into a strongly fluorescent compound using an electrolytic cell device. Detection parameters, such as potential and fluorescence wavelength, were optimized. The linearity of the proposed method ranged from 0.01 to 5.0 mg l−1 with a correlation coefficient of 0.9997. The limit of qualification (S/N = 10) and limit of detection (S/N = 3) were 9.7 and 2.9 µg l−1, respectively. Resperine recoveries from spiked blank and drug-treated mouse serum samples ranged from 92.0 to 115%.

show abstract