Simultaneous Determination of Reserpine and Hydrochlorothiazide in Two-Component Tablet Formulations by High-Performance Liquid Chromatography

Butterfield, A.G.; Lovering, Edward G; Sears, R.W.

doi:10.1002/jps.2600670520

Cited by 16 publications

(1 citation statement)

References 18 publications

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“…Simultaneous estimation of RES and HCTZ in tablet dosage form was performed by high-performance liquid chromatography (HPLC) using polythiazide as internal standard [ 6 ]. Simultaneous estimation of HCTZ and H.HCl was done by HPLC using dibutylamine phosphate buffer and ACN as mobile phase [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Estimation of Hydrochlorothiazide, Hydralazine Hydrochloride, and Reserpine Using PCA, NAS, and NAS-PCA

2015

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In this study, new and feasible UV-visible spectrophotometric and multivariate spectrophotometric methods were described for the simultaneous determination of hydrochlorothiazide (HCTZ), hydralazine hydrochloride (H.HCl), and reserpine (RES) in combined pharmaceutical tablets. Methanol was used as a solvent for analysis and the whole UV region was scanned from 200–400 nm. The resolution was obtained by using multivariate methods such as the net analyte signal method (NAS), principal component analysis (PCA), and net analyte signal-principal component analysis (NAS-PCA) applied to the UV spectra of the mixture. The results obtained from all of the three methods were compared. NAS-PCA showed a lot of resolved data as compared to NAS and PCA. Thus, the NAS-PCA technique is a combination of NAS and PCA methods which is advantageous to obtain the information from overlapping results.

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Section: Introductionmentioning

confidence: 99%