Fluorimetric detection of reserpine in mouse serum through online post-column electrochemical derivatization

Abstract

A novel method combining high-performance liquid chromatography with online post-column electrochemical derivatization and fluorescence detection was established for the detection of reserpine in mouse serum. Reserpine separation was conducted using a C18 column with 5 mM H3PO4 and acetonitrile (55/45, v/v) as eluent. Reserpine was then electro-oxidized into a strongly fluorescent compound using an electrolytic cell device. Detection parameters, such as potential and fluorescence wavelength, were optimized. The linearity of the proposed method ranged from 0.01 to 5.0 mg l−1 with a correlation coefficient of 0.9997. The limit of qualification (S/N = 10) and limit of detection (S/N = 3) were 9.7 and 2.9 µg l−1, respectively. Resperine recoveries from spiked blank and drug-treated mouse serum samples ranged from 92.0 to 115%.

An Immunosensor for the Detection of ULBP2 Biomarker

“…For our biosensor, the input signal is the amount of concentration of analyte present in the measuring medium, and the output signal is the impedance response at different frequencies using the EIS technique. The reported limit of detection (LOD) is the lowest concentration of an analyte in a sample that can be detected by the biosensor, and it was calculated using the following equation: LOD = 3 , where is the standard deviation of the response, and b is the slope of the linear regression line [ 27 , 28 , 29 ].…”