Fluorometric assay of histamine in tissues and body fluids: Choice of the purification procedure and identification in the nanogram range

An improved method for the determination of histamine release in man: Its application in studies with propanidid and thiopentone

“…below 3 ng/ml). The high 'histamine values' were due to substances fluorescing after condensation with o-phthaldialdehyde which were not, or not sufficiently, separated from histamine during the purification procedure (Lorenz et al, 1970a). The less than satisfactory methods used in previous investigations makes necessary revision of all results on histamine release in humans which are based upon plasma histamine assay.…”

“…The whole supernatant was filtered through a filter paper and was either used immediately for histamine assay or stored in the freezer at -20°C, and histamine assayed several days later. Histamine was determined on a Zeiss spectrofluorometer ZFM 4 at a temperature of 21-+0.5°C according to Lorenz et al (1970aLorenz et al ( , 1972) (combined method).…”

“…However, with an improved, highly sensitive and specific method for plasma histamine determination (Lorenz et al, 1970a;Lorenz et al, 1972) it has been possible, to show histamine release in man by 2 anesthetic drugs often used in European clinics: propanidid and thiopentone. Recent information of anaphylactoid incidents during anesthesia with both substances led us to this investigation.…”

Histamine and peptic ulcer: A prospective study of mucosal histamine concentration in duodenal ulcer patients and in control subjects suffering from various gastrointestinal diseases

“…Histamine was determined according to a modification of our Dowex-method (Lorenz et al, 1970). The modification was necessary in order to increase the sensitivity of the assay for measuring the amine only in a few milligrams of tissue .…”

“…Using a modification of the fluorometric method for histamine determination in tissues (Lorenz et al, 1970), this amine could be determined in all gastric biopsy specimens investigated Lorenz et al, 1976b). The specificity of the assay has been assured by the usual tests of identity (Lorenz, 1975;Lorenz et al, 1976), while the precision and relative accuracy of this technique have been shown by carrying out quality control experiments within the day and from day to day Rohde et al, 1976b).…”