Fluorogenic “Photoclick” Labeling and Imaging of DNA with Coumarin-Fused Tetrazole in Vivo

Wu, Yi; Guo, Guanlun; Zheng, Judun; Xing, Da; Zhang, Tao

doi:10.1021/acssensors.8b00565

Cited by 43 publications

(47 citation statements)

References 53 publications

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“… 8 A TMPRSS2-expressing VeroE6 cell line is highly susceptible to SARS-CoV-2 infection, making it ideal for virus isolation and cytopathic testing. 18 Photoclickable fluorogenic probes enable the visualization of specific DNA or RNA with precise spatiotemporal control in their native cellular environment, 19 facilitating the diagnosis of the viral cytopathic effect. However, virus isolation and visualization are impractical for large-scale diagnosis of COVID-19, since SARS-CoV-2 requires at least 3 days to inflict obvious cytopathic effects on selected cell lines.…”

Section: Diagnostic Approaches To Covid-19mentioning

confidence: 99%

Current and Perspective Diagnostic Techniques for COVID-19

et al. 2020

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Since late December 2019, the coronavirus pandemic (COVID-19; previously known as 2019-nCoV) caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been surging rapidly around the world. With more than 1,700,000 confirmed cases, the world faces an unprecedented economic, social, and health impact. The early, rapid, sensitive, and accurate diagnosis of viral infection provides rapid responses for public health surveillance, prevention, and control of contagious diffusion. More than 30% of the confirmed cases are asymptomatic, and the high false-negative rate (FNR) of a single assay requires the development of novel diagnostic techniques, combinative approaches, sampling from different locations, and consecutive detection. The recurrence of discharged patients indicates the need for long-term monitoring and tracking. Diagnostic and therapeutic methods are evolving with a deeper understanding of virus pathology and the potential for relapse. In this Review, a comprehensive summary and comparison of different SARS-CoV-2 diagnostic methods are provided for researchers and clinicians to develop appropriate strategies for the timely and effective detection of SARS-CoV-2. The survey of current biosensors and diagnostic devices for viral nucleic acids, proteins, and particles and chest tomography will provide insight into the development of novel perspective techniques for the diagnosis of COVID-19.

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Section: Diagnostic Approaches To Covid-19mentioning

confidence: 99%

Current and Perspective Diagnostic Techniques for COVID-19

et al. 2020

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“…Live cell labelling of DNA was reported to be efficient and fluorogenic with a water-soluble tetrazole and upon irradiation by 350 nm light. 115 …”

Section: Discussionmentioning

confidence: 99%

Labelling of DNA and RNA in the cellular environment by means of bioorthogonal cycloaddition chemistry

2020

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“…VdU was also used in combination with the fluorogenic photo-click reaction. 212 Here, a coumarin-fused tetrazol and UV irradiation led to DNA labeling in cells and zebrafish. A recent addition to nucleosides for metabolic DNA labeling is vinyl thioether-modified thymidine (VTdT, Fig.…”

Section: Feeding Nucleoside Pre-cursorsmentioning

confidence: 97%