Fluorochloridone perturbs blood-testis barrier/Sertoli cell barrier function through Arp3-mediated F-actin disruption

Liu, Luqing; Zhang, Yubin; Chang, Xiuli; Li, Rui; Wu, Chunhua; Tang, Liming; Zhou, Zhijun

doi:10.1016/j.toxlet.2018.07.001

Cited by 19 publications

(11 citation statements)

References 20 publications

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“…The current study demonstrated that the integrity of tight junction in rat testis of ADR-treated group was substantially damaged. Tight junction proteins such as cloudin-11, occludin, and ZO1 play a critical role in maintaining BTB integrity [40]. Expression levels of BTB junction proteins claudin-11, occludin, and ZO1 were downregulated in the ADR group.…”

Section: Discussionmentioning

confidence: 99%

Cross-talk between ER stress and mitochondrial pathway mediated adriamycin-induced testicular toxicity and DA-9401 modulate adriamycin-induced apoptosis in Sprague–Dawley rats

et al. 2019

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Background DA-9401 was prepared as a mixture of Chinese medicinal herb extracts from roots of Morinda officinalis How (Rubiaceae), outer scales of Allium cepa L. (Liliceae) and seeds of Cuscuta chinensis Lamark (Convolvulaceae). The present study was designed to investigate the possible protective role of DA-9401 in adriamycin (ADR)-induced testicular toxicity associated with oxidative stress, endoplasmic reticulum (ER) stress, and apoptosis. Methods Fifty healthy 8-week-old male Sprague–Dawley rats were equally divided into five groups. The first CTR group was treated with normal saline 2 ml/day by gavage. The second was treated with DA-100 (DA-9401 100 mg/kg/day). The third (ADR) group received ADR (2 mg/kg/once a week) intraperitoneally, while the combination of ADR and DA-9401 was given to the fourth ADR + DA-100 (100 mg/kg/day p.o) group and fifth ADR + DA-200 (200 mg/kg/day p.o) group. At the end of the 8-week treatment period, body weight, reproductive organ weights, fertility rate, pups per female were recorded, and serum were assayed for hormone concentrations. Tissues were subjected to semen analysis, histopathological changes, interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), oxidative stress markers and expression levels of endoplasmic reticulum (ER) stress markers, apoptosis markers, tight junction protein markers, steroidogenic acute regulatory protein (StAR), cation channel of sperm (CatSper) and glycogen synthase kinase-3 (GSK-3) by western blot. Results DA-9401 administration to ADR-treated rats significantly decreased serum luteinizing hormone (LH) and follicle-stimulating hormone (FSH) levels, interleukin-6, TNF-α, MDA level, ROS/RNS level, ER stress response protein levels, tunnel positive cells, cleaved caspase-3, and Bax/Bcl2 ratio. Moreover, pretreatment with DA-9401 significantly increased body weight, reproductive organ weights, fertility rate, pups per female, Johnsen’s score, spermatogenic cell density, sperm count and sperm motility, serum testosterone concentration, testicular superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx), tight junction protein markers, star protein level, CatSper, and GSK-3 level. Conclusions ADR treatment can markedly impair testicular function and induce testicular cell death presumably by causing significant changes in oxidative stress, ER stress, and mitochondrial pathway. DA-9401 exerts beneficial effects against oxidative stress, ER stress, and mitochondria-mediated cell death pathway in testis tissue by up-regulating expression levels of tight junction protein markers, steroidogenic acute regulatory protein, GSK-3 alpha, and cation channels of sperm. Electronic supplementary material The online version of this article (10.1186/s12935-019-0805-2) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Cross-talk between ER stress and mitochondrial pathway mediated adriamycin-induced testicular toxicity and DA-9401 modulate adriamycin-induced apoptosis in Sprague–Dawley rats

et al. 2019

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“…To our knowledge, there is no study in the literature showing the effect of epileptic seizures on blood–testis barrier. Other studies showed decreased expression of ZO‐1 after exposure to low‐dose radiation (Son et al., 2015), flutamide (Chojnacka et al., 2016), fluorochloridone (Liu et al., 2018) and bisphenol A and diethylhexylphthalate (Salian et al., 2009; Sobarzo et al., 2009). In the present study, it was observed that ZO‐1 immunoreactivity was decreased and even absent in some areas in the seminiferous tubules, and the structure of blood‐testis barrier was disrupted after PTZ‐induced seizures.…”

Section: Discussionmentioning

confidence: 95%

Histological analysis of the effects of thymoquinone on testicular damage in pentylenetetrazole‐induced temporal lobe epilepsy model

2021

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Epilepsy is a neurological disease, affecting approximately 50 million people, with a prevalance of 1% in the world (Shokri et al., 2015;Zupec-Kania & Spellman, 2008). Temporal lobe epilepsy is the most common form of this disease (Engel, 2001).Reproductive and sexual function disorders are frequent in male and female patients with epilepsy (Luef & Madersbacher, 2015;Webber et al., 1986). 90% of males with epilepsy were reported to have abnormal sperm count, morphology and motility (Montouris & Morris, 2005). Both epilepsy and antiepileptic drugs play a role in the pathophysiology of reproductive disorders (Herzog, 2008;Rattya et al., 2001). Studies have shown alterations in endocrine system and hormonal levels as a result of epileptiform activity (Hauser & Beghi, 2008;Veliskova & Desantis, 2013). Abnormal serum testosterone and gonadotropin concentrations, abnormal LH response to GnRH stimulation and increased serum prolactin levels were reported to be related to epileptic discharges (Montouris & Morris, 2005).Nigella sativa (N. sativa), belonging to Ranunculaceae family, is a plant frequently used in modern and alternative medicine (Bamosa et al., 2015). Thymoquinone (C 10 H 12 O 2 , 2-isopropyl-5-methyl 1, 4-benzoquinone) is the most important bioactive compound found

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“…This disrupting effect appears to be mediated initially by changes in the expression and localization of actin bundling proteins ezrin and fascin 1 in Sertoli cells. We have already known that actin bundling proteins leads to regulate germ cell migration during spermatogenesis [22]. These changes, in turn, perturb F-actin organization at the BTB, rendering actin filament bundles, the hallmark ultrastructure of the BTB in mammalian testes [23], which are unable to assume their bundled configuration to support BTB integrity.…”

Section: Discussionmentioning

confidence: 99%

PFOS ile tedavi edilen Sertoli hücre kültüründe ezrin ve fascin 1'in araştırılması: İn vitro bir çalışma

Güngör‐Ordueri

2020

Acta Medica Alanya

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Amaç: Bu çalışmada, Ezrin ve Fascin 1'in in vivo olarak baskılanması sonucundaki bulgularla birlikte, Kan testis bariyeri (KTB) yapısının bozulması ve primer Sertoli hücre kültüründe, Perflorooktansülfonat (PFOS) muamelesinden sonra F-aktin demetlenmesinin gösterilmesi amaçlanmaktadır. Çalışma Tasarımı: Primer Sertoli hücre izolasyonu, kontrol ve PFOS ile muamele edilen (20μM) gruplarla yapıldı. Sertoli hücre konsantrasyonu her iki deney için 0.5 x 106 hücre/ml olacak şekilde hazırlandı. Metod: Hem Fascin 1'in Sertoli hücrelerinde Ezrin ile birlikte lokalizasyonunu değerlendirmek üzere çift etiketli immünofloresan analizi yapıldı, hem de seminifer tübül lizatlarından aktin ve Ezrin proteinlerinin Fascin 1 proteini ile spesifik protein-protein etkileşimini tanımlamak için Co-IP deneyi uygulandı. Bulgular: İlk olarak, ektoplazma özelleşmesi bileşenleri olan Ezrin ve Fascin 1 in Sertoli hücrelerinde ko-lokalize olduğu ve birbirleri ile etkileşime girdiği gösterilmiştir. İkinci olarak ise PFOS ile muamele edilen Sertoli hücrelerinde Ezrin ve Fascin 1 dislokasyonu gösterilmiştir. 20μM PFOS uygulandığında aktin tabanlı hücre iskeleti KTB'deki hüce-hücre bağlantı bölgelerindeki aktin düzenleyici proteinlerin yayılmasını ve/veya lokalizasyonunu destekleyememiştir. Tartışma: Sonuç olarak bu bulgular, PFOS aracılı Sertoli hücre bozulmasında, Ezrin ve Fascin 1'in KTB bütünlüğünü korumak için F-aktin organizasyonunu düzenleyerek birlikte çalışabileceğini desteklemektedir.

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Fluorochloridone perturbs blood-testis barrier/Sertoli cell barrier function through Arp3-mediated F-actin disruption

Cited by 19 publications

References 20 publications

Cross-talk between ER stress and mitochondrial pathway mediated adriamycin-induced testicular toxicity and DA-9401 modulate adriamycin-induced apoptosis in Sprague–Dawley rats

Cross-talk between ER stress and mitochondrial pathway mediated adriamycin-induced testicular toxicity and DA-9401 modulate adriamycin-induced apoptosis in Sprague–Dawley rats

Histological analysis of the effects of thymoquinone on testicular damage in pentylenetetrazole‐induced temporal lobe epilepsy model

PFOS ile tedavi edilen Sertoli hücre kültüründe ezrin ve fascin 1'in araştırılması: İn vitro bir çalışma

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