Histological analysis of the effects of thymoquinone on testicular damage in pentylenetetrazole‐induced temporal lobe epilepsy model

Abstract

Epilepsy is a neurological disease, affecting approximately 50 million people, with a prevalance of 1% in the world (Shokri et al., 2015;Zupec-Kania & Spellman, 2008). Temporal lobe epilepsy is the most common form of this disease (Engel, 2001).Reproductive and sexual function disorders are frequent in male and female patients with epilepsy (Luef & Madersbacher, 2015;Webber et al., 1986). 90% of males with epilepsy were reported to have abnormal sperm count, morphology and motility (Montouris & Morris, 2005). Both epilepsy and antiepileptic drugs play a role in the pathophysiology of reproductive disorders (Herzog, 2008;Rattya et al., 2001). Studies have shown alterations in endocrine system and hormonal levels as a result of epileptiform activity (Hauser & Beghi, 2008;Veliskova & Desantis, 2013). Abnormal serum testosterone and gonadotropin concentrations, abnormal LH response to GnRH stimulation and increased serum prolactin levels were reported to be related to epileptic discharges (Montouris & Morris, 2005).Nigella sativa (N. sativa), belonging to Ranunculaceae family, is a plant frequently used in modern and alternative medicine (Bamosa et al., 2015). Thymoquinone (C 10 H 12 O 2 , 2-isopropyl-5-methyl 1, 4-benzoquinone) is the most important bioactive compound found

Ameliorative Potentials of Bombax costatum Pellegr. & Vuillet Chloroform Stem Bark Extract on Pentylenetetrazole-induced Testicular Damage in Wistar Rats

“…Also, tubular vacuolization with the lumen empty of spermatozoa noticed. This has been supported in a study carried out by (Karakaya et al, 2021) where PTZ administration resulted in a severe testicular tissue damage. The histological appearance of the group that was given BC followed by PTZ (Figure 2c) showed no changes in their tubular architecture when compared with the control group.…”

Testicular Apoptosis and Proliferation in Relation to Body Mass Index and m-TESE Success in Nonobstructive Azoospermic Men

“…Proliferative index (PI) was calculated by counting the positive cells in 10 randomly selected seminiferous tubules from a section of each sample at 400x magnification under the microscope and dividing them by the total number of cells (PI: PCNA positive cell count/total cell count × 100%) [28]. The sections were examined and photographed with an Olympus DP72 camera-attached Olympus BX51 light microscope under ×40 objective.…”