Fluorescent Sensors Based on Aptamer Self-Assembly

Stojanović, Milan N.; Prada, Paloma de; Landry, Donald W.

doi:10.1021/ja0022223

Cited by 416 publications

(448 citation statements)

References 12 publications

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“…A conventional three-electrode system was used: a gold electrode of interest as working electrode, a saturated calomel electrode (SCE) as reference electrode, and platinum foil as auxiliary electrode. Cyclic voltammetry (CV) was implemented in 5 mL of 10 mM PBS (pH 7.4) containing 5 mM Fe(CN) 6 3-/Fe(CN) 6 4-and 0.1 M KCl at a scan rate of 100 mV/s. For target protein detection, 20 µL of PDGF-BB solution with a certain concentration was placed onto the sensing interface to incubate for 1 h. After rinsing with the stock buffer, AC voltammogram was performed in 1M NaClO 4 at a frequency of 1 Hz, and the peak current was used to evaluate the response characteristics of sensing interface.…”

Section: Methodsmentioning

confidence: 99%

“…Recently, a new class of recognition element for proteins named aptamer has been widely studied due to its numerous advantages such as good stability, simple synthesis, easy labeling and high affinity and specificity when binding with targets [3]. Lots of aptamer based biosensing methods have been delevoped including colorimetric, fluorescent, electrochemical, and so on [4][5][6][7][8]. Thereinto the electrochemical strategies have attracted the most attention because of their numerous merits including high sensitivity, low cost, small dimensions and compatibility with microfabrication technology [9,10].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Structure-switching hairpin probe based electrochemical aptasensor for highly sensitive detection of protein

Hu¹,

Zhang²,

Lü³

et al. 2015

Proceedings of the 5th International Conference on Advanced Design and Manufacturing Engineering

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Abstract. Using platelet derived growth factor B chain (PDGF-BB) as the model analyte, a structure-switching hairpin probe based electrochemical biosensing strategy for highly sensitive detection of protein is proposed in this work. Aptamer probe hybridized with the immobilized hairpin structured signal probe to form DNA duplex. Upon binding with target protein, competition between target molecule and the signal probe with the aptamer probe happened, inducing the signal probe from stretched duplex to hairpin structure. Under optimum experimental conditions, the target protein can be sensitively detected in a linear dynamic range from 0.1 ng/mL to 500 ng/mL with a low detection limit of 0.08 ng/mL.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Structure-switching hairpin probe based electrochemical aptasensor for highly sensitive detection of protein

Hu¹,

Zhang²,

Lü³

et al. 2015

Proceedings of the 5th International Conference on Advanced Design and Manufacturing Engineering

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show abstract

“…It can be split into two halves and binding still occurs when both fragments are present ( Figure 1B). [39][40][41][42][43] We first tested the effect of molecular imprinting if both halves were used as macromonomers. Each aptamer fragment was labeled on the 5-end with an acrydite group to co-polymerize into the gel.…”

Section: Molecular Imprinting Helps Aptamer Bindingmentioning

confidence: 99%

Molecularly Imprinted Polymers with DNA Aptamer Fragments as Macromonomers

Zhang

Liu

2016

ACS Appl. Mater. Interfaces

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Molecularly imprinted polymers (MIPs) are produced in the presence of a template molecule. After removing the template, the cavity can selectively rebind the template. MIPs are attractive functional materials with a low cost and high stability, but traditional MIPs often suffer from low binding affinity. This study employs DNA aptamer fragments as macromonomers to improve MIPs. The DNA aptamer for adenosine was first split into two halves, fluorescently labeled, and co-polymerized into MIPs. With a fluorescence quenching assay, the importance of imprinting was confirmed. Further studies were carried out using isothermal titration calorimetry (ITC).Compared to the mixture of the free aptamer fragments, their MIPs doubled the binding affinity.Each free aptamer fragment alone cannot bind adenosine, while MIPs containing each fragment are effective binders. We further shortened one of the aptamer fragments, and the DNA length was pushed to as short as six nucleotides, yielding MIPs with a dissociation constant of 27 µM adenosine. This study provides a new method for preparing functional MIP materials by combining high affinity biopolymer fragments with low cost synthetic monomers, allowing higher binding affinity and providing a method for signaling binding based on DNA chemistry.

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“…In the last decades, aptamers have attracted tremendous interest in therapeutic and bioanalytical applications, either used as an active separation material in chromatography or as recognition material in biosensing applications to replace commonly-used bio-receptors [40][41][42][43][44][45]. Aptamers are synthetic oligonucleotides sequences (30-100 nucleotides) with high affinity and specificity to recognize their cognate target molecules, ranging from small ions to large peptides.…”

Section: Aptamermentioning

confidence: 99%

Recent Advances in Electrochemical-Based Sensing Platforms for Aflatoxins Detection

et al. 2016

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Abstract:Mycotoxin are small (MW~700 Da), toxic secondary metabolites produced by fungal species that readily colonize crops and contaminate them at both pre-and post-harvesting. Among all, aflatoxins (AFs) are mycotoxins of major significance due to their presence in common food commodities and the potential threat to human health worldwide. Based on the severity of illness and increased incidences of AFs poisoning, a broad range of conventional and analytical detection techniques that could be useful and practical have already been reported. However, due to the variety of structural analogous of these toxins, it is impossible to use one common technique for their analysis. Numerous recent research efforts have been directed to explore alternative detection technologies. Recently, immunosensors and aptasensors have gained promising potential in the area of sample preparation and detection systems. These sensors offer the advantages of disposability, portability, miniaturization, and on-site analysis. In a typical design of an aptasensor, an aptamer (ssDNA or RNA) is used as a bio-recognition element either integrated within or in intimate association with the transducer surface. This review paper is focused on the recent advances in electrochemical immunoand aptasensing platforms for detection of AFs in real samples.

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Fluorescent Sensors Based on Aptamer Self-Assembly

Cited by 416 publications

References 12 publications

Structure-switching hairpin probe based electrochemical aptasensor for highly sensitive detection of protein

Structure-switching hairpin probe based electrochemical aptasensor for highly sensitive detection of protein

Molecularly Imprinted Polymers with DNA Aptamer Fragments as Macromonomers

Recent Advances in Electrochemical-Based Sensing Platforms for Aflatoxins Detection

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