Fluorescent probe based on carbon dots/silica/molecularly imprinted polymer for lysozyme detection and cell imaging

Fang, Mengyao; Zhuo, Kelei; Chen, Yujuan; Zhao, Yajing; Bai, Guangyue; Wang, Jianji

doi:10.1007/s00216-019-01960-6

Cited by 41 publications

(16 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As a result, the stable and reusable properties have increased the long shelf-life usability of MIPs. Some studies reported the shelf life of MIPs is a minimum of 6 weeks [132] and a maximum of 6 [133] to 12 months [134] for MIP-based biosensors stored at 25 • C. This method is considered a versatile and hopeful technique that can recognize both biological and chemical analytes, including nucleotides [135], amino acids [136], enzymes [137], proteins [138], phosphoproteins [139], viruses [140], bacteria [141], pollutants [142], dyes [143], food poisons [144], pesticides [145], and toxins [146,147].…”

Section: Molecular Imprinting Techniquementioning

confidence: 99%

Recent Advances in Quartz Crystal Microbalance Biosensors Based on the Molecular Imprinting Technique for Disease-Related Biomarkers

2022

View full text Add to dashboard Cite

The molecular imprinting technique is a quickly developing field of interest regarding the synthesis of artificial recognition elements that enable the specific determination of target molecule/analyte from a matrix. Recently, these smart materials can be successfully applied to biomolecule detection in biomimetic biosensors. These biosensors contain a biorecognition element (a bioreceptor) and a transducer, like their biosensor analogs. Here, the basic difference is that molecular imprinting-based biosensors use a synthetic recognition element. Molecular imprinting polymers used as the artificial recognition elements in biosensor platforms are complementary in shape, size, specific binding sites, and functionality to their template analytes. Recent progress in biomolecular recognition has supplied extra diagnostic and treatment methods for various diseases. Cost-effective, more robust, and high-throughput assays are needed for monitoring biomarkers in clinical settings. Quartz crystal microbalance (QCM) biosensors are promising tools for the real-time and quick detection of biomolecules in the past two decades A quick, simple-to-use, and cheap biomarkers detection technology based on biosensors has been developed. This critical review presents current applications in molecular imprinting-based quartz crystal microbalance biosensors for the quantification of biomarkers for disease monitoring and diagnostic results.

show abstract

Section: Molecular Imprinting Techniquementioning

confidence: 99%

Recent Advances in Quartz Crystal Microbalance Biosensors Based on the Molecular Imprinting Technique for Disease-Related Biomarkers

2022

View full text Add to dashboard Cite

show abstract

“…Thus, the use of carbon-based materials (CBM) as a matrix provides high versatility on the selection of polymers and high robustness to the final sensor. Different types of CBM have been explored in the synthesis of MIPs, such as graphene oxide [ 85 , 86 , 87 ], carbon dots [ 88 , 89 , 90 , 91 , 92 ], and carbon nanotubes [ 93 , 94 , 95 , 96 , 97 ]. Most of these application employs CBM as a substrate to support the MIP, that can be synthesized in different shapes and sizes, depending on the selected CBM.…”

Section: Analytical Applications Of Mip Nanoparticlesmentioning

confidence: 99%

Molecularly Imprinted Polymer Micro- and Nano-Particles: A Review

2020

View full text Add to dashboard Cite

In recent years, molecularly imprinted polymers (MIPs) have become an excellent solution to the selective and sensitive determination of target molecules in complex matrices where other similar and relative structural compounds could coexist. Although MIPs show the inherent properties of the polymers, including stability, robustness, and easy/cheap synthesis, some of their characteristics can be enhanced, or new functionalities can be obtained when nanoparticles are incorporated in their polymeric structure. The great variety of nanoparticles available significantly increase the possibility of finding the adequate design of nanostructured MIP for each analytical problem. Moreover, different structures (i.e., monolithic solids or MIPs micro/nanoparticles) can be produced depending on the used synthesis approach. This review aims to summarize and describe the most recent and innovative strategies since 2015, based on the combination of MIPs with nanoparticles. The role of the nanoparticles in the polymerization, as well as in the imprinting and adsorption efficiency, is also discussed through the review.

show abstract

“…Lysozyme is a native protein with ellipsoidal structure due to the interaction of hydrophobic groups [ 32 ]. The lysozyme level in body fluid is closely related to leukemia [ 33 ], renal disease [ 34 ], meningitis [ 35 ] and other diseases [ 36 ]. For example, the increase of lysozyme concentration in urine may be caused by renal tubular cell damage [ 37 ].…”

Section: Introductionmentioning

confidence: 99%

Multi-Factors Cooperatively Actuated Photonic Hydrogel Aptasensors for Facile, Label-Free and Colorimetric Detection of Lysozyme

Shen

Shi

et al. 2022

Biosensors

View full text Add to dashboard Cite

Responsive two-dimensional photonic crystal (2DPC) hydrogels have been widely used as smart sensing materials for constructing various optical sensors to accurately detect different target analytes. Herein, we report photonic hydrogel aptasensors based on aptamer-functionalized 2DPC poly(acrylamide-acrylic acid-N-tert-butyl acrylamide) hydrogels for facile, label-free and colorimetric detection of lysozyme in human serum. The constructed photonic hydrogel aptasensors undergo shrinkage upon exposure to lysozyme solution through multi-factors cooperative actuation. Here, the specific binding between the aptamer and lysozyme, and the simultaneous interactions between carboxyl anions and N-tert-butyl groups with lysozyme, increase the cross-linking density of the hydrogel, leading to its shrinkage. The aptasensors’ shrinkage decreases the particle spacing of the 2DPC embedded in the hydrogel network. It can be simply monitored by measuring the Debye diffraction ring of the photonic hydrogel aptasensors using a laser pointer and a ruler without needing sophisticated apparatus. The significant shrinkage of the aptasensors can be observed by the naked eye via the hydrogel size and color change. The aptasensors show good sensitivity with a limit of detection of 1.8 nM, high selectivity and anti-interference for the detection of lysozyme. The photonic hydrogel aptasensors have been successfully used to accurately determine the concentration of lysozyme in human serum. Therefore, novel photonic hydrogel aptasensors can be constructed by designing functional monomers and aptamers that can specifically bind target analytes.

show abstract

Fluorescent probe based on carbon dots/silica/molecularly imprinted polymer for lysozyme detection and cell imaging

Cited by 41 publications

References 43 publications

Recent Advances in Quartz Crystal Microbalance Biosensors Based on the Molecular Imprinting Technique for Disease-Related Biomarkers

Recent Advances in Quartz Crystal Microbalance Biosensors Based on the Molecular Imprinting Technique for Disease-Related Biomarkers

Molecularly Imprinted Polymer Micro- and Nano-Particles: A Review

Multi-Factors Cooperatively Actuated Photonic Hydrogel Aptasensors for Facile, Label-Free and Colorimetric Detection of Lysozyme

Contact Info

Product

Resources

About