1986
DOI: 10.3147/jsfp.21.39
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Fluorescent antibody technique compared to standard media culture for detection of pathogenic bacteria for yellowtail and amberjack.

Abstract: During a one year period, 355 net pen cultured yellowtail (Seriola quinqueradiata) and amber jack (Seriola dumerili) mortalities were examined for bacterial infections. A comparison of detec tion of pathogenic bacteria by direct fluorescent antibody bechnique (FAT) and media culture was performed.Fluorescein labeled rabbit immunoglobulin G (IgG : Fl) was prepared against Streptococcus sp., Pasteurella piscicida, Nocardia kampachi and Vibrio anguillarum . Heat fixed smears of kidney from each mortality were sta… Show more

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Cited by 14 publications
(7 citation statements)
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“…There have been a few reports de scribing the sensitivity and specificity of FAT to the pathogens from infected fishes (KAWAHARA et al, 1986;KUSUDA and KAWAHARA, 1987).…”
Section: Discussionmentioning
confidence: 99%
“…There have been a few reports de scribing the sensitivity and specificity of FAT to the pathogens from infected fishes (KAWAHARA et al, 1986;KUSUDA and KAWAHARA, 1987).…”
Section: Discussionmentioning
confidence: 99%
“…Indeed, P. damselae subsp. piscicida has been mentioned in the past as a significant pathogen of greater amberjack in Japan (Kubota et al, 1970; Kawahara et al, 1986), although it has not been yet isolated from its Mediterranean counterpart. Notably, the pathogen has been experimentally transferred, producing the common characteristics of the disease in challenged greater amberjack farmed in the Mediterranean region (Alcaide et al, 2000).…”
Section: Bacteriamentioning
confidence: 99%
“…Todd Hewitt medium, brain heart infusion or heart infusion media containing 0.5% glucose at 25-30°C are normally used for the isolation of the bacteria in the laboratory. A definite diagnosis requires serological typing and fluorescent antibody techniques (Kawahara et al 1986). …”
Section: Diagnosismentioning
confidence: 99%