Fluorescent-Antibody Approach to Study of Rhizobia in Soil

Schmidt, E. L.; Bankole, Rufus O.; Bohlool, B. Ben

doi:10.1128/jb.95.6.1987-1992.1968

Cited by 197 publications

(82 citation statements)

References 15 publications

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“…Strains of rhizobia were obtained as described (9) and have been carried in our laboratory for several years, as were the other cultures used in this study. The usual culture medium for the rhizobia was a modified yeast extract-mannitol-salts solution (1).…”

Section: Methodsmentioning

confidence: 99%

Immunofluorescent polar tips of Rhizobium japonicum: possible site of attachment or lectin binding

Bohlool¹,

Schmidt²

1976

J Bacteriol

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Rhizobium japonicum USDA 31 demonstrated marked polarity by binding homologous fluorescent antibody (FA) heavily on one end of the cell. FA prepared against R. japonicum strains 110 and 138, and against R. trifolii TA1 cross-reacted with strain 31 only in the polar tip region. No polar immunofluorescing tips could be seen with FA against two other strains of R. japonicum or with those against several unrelated microorganisms. Common antigens localized only in a polar region were seen in many rhizobia stained with R. japonicum 31 FA: 22 of 23 strains of R. japonicum, 10 of 17 strains of R. trifolii, 3 of 7 strains of R. melitolii, 3 of 6 strains of R. phaseoli, and 3 of 9 strains of R. leguminosarum had some cells with detectable polar tips. The proportion of R. japonicum 31 cells with polar tips was high throughout the growth cycle. Polar tip staining was not affected by drastic cell treatments. A function was proposed for the polar tip region as a site for attachment. R. japonicum 31 cells attached to each other in a tip-to-tip fashion and endwise to fungal hyphae with the polar tip in contact with the hyphal wall. Binding of fluorescein isothiocyanate-labeled soybean lectin to certain strains of R. japonicum gave additional evidence of polarity. Polar binding of both antibody and lectin may provide insights into relationships between rhizobia and roots of host legumes.

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Section: Methodsmentioning

confidence: 99%

Immunofluorescent polar tips of Rhizobium japonicum: possible site of attachment or lectin binding

Bohlool¹,

Schmidt²

1976

J Bacteriol

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“…Thus, by the use of FAT soil ecologists have been able to detect soil fungi such as Aspergillusjavus (Schmidt & Bankole, 1962), Arthrobotrys conoides (Eren & Pramer, 1966) ancl soil bacteria, like Bacillusspp. (Hill & Gray, 1967) and Rhizobium japonicum (Schmidt, Bankole & Bohlool, 1968). To date, this method has not been applied to detect and identify animal pathogens in soil.…”

Section: Discussionmentioning

confidence: 99%

On the Growth and Survival of Clostridium septicum in Soil

García¹,

McKay²

1969

Journal of Applied Bacteriology

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Clostridium septicum was inoculated into soils treated in different ways and samples were removed periodically for microbial anaIyses by one or more of the following methods : plate counts, fluorescent antibody staining, dehydrogenase activity and manometric techniques. Mass sporulation of the vegetative cells was not triggered initially when inoculated into amended soils. Moreover, the vegetative forms persisted in both sterile and unsterile soils for up to 30 days in incubated samples. Glucose, pyruvate, peptone and manure enhanced the growth and metabolic activity of CZ. septicuw in soil. Wyoming bentonite tended to decrease dehydrogenase activity whereas Georgia kaolinite had no detectable effect. The significance of organic matter addition and type of clay mineral on the ecology of CZ. septicurn is discussed. . (1949). The occurrence of vegetative cells of Clostridium perfringem in soils.

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“…Fluorescent-antibody studies. Preparation of fluorescent-antibody against purified B. stearothermophilus cell walls was done by C. B. Fliermans (E. I. DuPont de Nemours, Aiken, S.C.), following the procedure of Schmidt et al (31). The antigen was suspended in phosphate-buffered saline to give a 1-mg/ ml concentration.…”

mentioning

confidence: 99%

Effect of restrictive temperature on cell wall synthesis in a temperature-sensitive mutant of Bacillus stearothermophilus

Mulks¹,

Souza²,

Boylen³

1980

J Bacteriol

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A temperature-sensitive mutant of Bacillus stearothermophilus, TS-13, was unable to grow above 58°C, compared to 720C for the wild type. Actively growing TS-13 cells lysed within 2 h when exposed to a restrictive temperature of 65°C. Peptidoglycan synthesis stopped within 10 to 15 min postshift before a shut down of other macromolecular syntheses. Composition of preexisting peptidoglycan was not altered, nor was new peptidoglycan of aberrant composition formed. No significant difference in autolysin activity was observed between the mutant and the wild type at 650C. Protoplasts of TS-13 cells were able to synthesize cell wall material at 520C, but not at 650C. This wall material remained closely associated 710C, with its temperature-sensitive mutant, TS-13, whose range is 37 to 580C. It has been shown (20, 35) that this temperature-sensitive mutant is unable to regulate both fatty acid and phospholipid metabolism in a normal fashion at high temperatures. Above the restrictive temperature of 580C, it undergoes lysis. We present here evidence that lysis in this mutant is caused by the cessation of cell wall synthesis due to 413 on August 1, 2020 by guest

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Fluorescent-Antibody Approach to Study of Rhizobia in Soil

Cited by 197 publications

References 15 publications

Immunofluorescent polar tips of Rhizobium japonicum: possible site of attachment or lectin binding

Immunofluorescent polar tips of Rhizobium japonicum: possible site of attachment or lectin binding

On the Growth and Survival of Clostridium septicum in Soil

Effect of restrictive temperature on cell wall synthesis in a temperature-sensitive mutant of Bacillus stearothermophilus

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