“…Traditional detection techniques, including mass spectrometry, high-performance liquid chromatography, and electrochemistry, have been widely applied in the detection of RSS. − However, these methods usually require sophisticated preprocessing of test samples, and their real-time imaging applicability is restricted by the long detection times and complicated techniques that they require. , Fluorescent probes, which are simple, highly selective and sensitive, non-invasive, non-destructive, and real-time capable in living systems, are considered to be an effective molecular tool for the detection and imaging of RSS in plant systems. − RSS-responsive fluorescent probes based on a variety of fluorescent materials, including inorganic materials, nanoparticles, graphene, and gene-encoded fluorescent detectors, have been extensively developed and widely used in the detection and imaging in biological systems. − In comparison to these materials, organic small-molecule fluorescent probes with structural diversity are easily modified to intensify the detecting performance, high reproducibility, and response mechanisms. − …”