Fluorescence spectra of blood and urine for cervical cancer detection

Abstract

In the current study, the fluorescence emission spectra (FES) and Stokes shift spectra (SSS) of blood and urine samples of cervical cancer patients were obtained and compared to those of normal controls. Both spectra showed that the relative intensity of biomolecules such as porphyrin, collagen, Nicotinamide adenine dinucleotide, and flavin were quite out of proportion in cervical cancer patients. The biochemical mechanism for the elevation of these fluorophores is not yet definitive; nevertheless, these biomolecules could serve as tumor markers for diagnosis, screening, and follow-up of cervical cancers. To the best of our knowledge, this is the first report on FES and SSS of blood and urine of cervical cancer patients to give a sensitivity of 80% and specificity of 78%.

Autofluorescence Imaging and Spectroscopy of Human Lung Cancer

“…Masilamani et al found that the ratio of I 630 nm /I 585 nm in blood or urine samples was about one for normal controls and 2-3 for advanced cervical cancer patients [51]. Similar findings were also reported in blood samples of gastric cancer and breast cancer patients [40].…”

Cervical cancer detection by time-resolved spectra of blood components

“…So the porphyrin concentration is more in the diseased blood components. [8][9][10] The porphyrin which gets accumulated abnormally on the tumor tissue experiences different local environment, such as aggregation, higher acidity and also bonding to the intercellular matrix. All these could lead to enhanced lifetime, 14,15 in tissue as shown by others and in blood, as shown in this paper.…”

“…As the steady-state fluorescence spectra for blood samples of cancer patients have been studied extensively, [8][9][10][11][12] the discussion will focus only on one pertinent observation. In the field of fluorescence spectroscopy, the relative intensities of different peaks are more important than the actual, absolute intensities.…”

“…We reported in our previous papers [8][9][10] on the results of steady-state measurements of blood components for cancer diagnosis using porphyrin as a biomarker. The results showed a significant discrimination accuracy to classify the diseased blood from normal blood.…”

Fluorescence spectral classification of iron deficiency anemia and thalassemia

“…4,5 Earlier, in a series of papers, we had shown the efficacy of spectral technique for diagnosis of different types of cancers. 6 As a logical extension, we demonstrate here the potential of spectral differentiation between IDA and β Thal patients. This method could prove useful to identify and separate IDA samples from those of Thal.…”