Cervical cancer detection by time-resolved spectra of blood components

Kalaivani, R.; Masilamani, V.; AlSalhi, Mohamad S.; Devanesan, Sandhanasamy; Ramamurthy, P.; Palled, Siddanna R.; Ganesh, K M

doi:10.1117/1.jbo.19.5.057011

Cited by 12 publications

(14 citation statements)

References 17 publications

(31 reference statements)

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“…These values are less for the control group but at their maximum for cancer patients. Normal porphyrin was elevated for the patients, consistent with previous results (see Table 4 ) ( Kalaivani et al, 2014 ). Weber and co-workers (2013) use the methods of fluorescence spectroscopy to examine and measure the uptake of DOX chemotherapeutic drug in human breast cancer cells.…”

Section: Discussionsupporting

confidence: 92%

Magnetic field potential effects on the doxorubicin therapeutic activity in Ehrlich tumor growth

Ghannam

Al-Otaibi

Alanazy

et al. 2021

Saudi Journal of Biological Sciences

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Aim Therapeutic choices for cancer patients include many combinations of therapeutic protocols. The present study aimed to investigate and discuss the combined effects of magnetic field and chemotherapy treatment on Ehrlich tumor-induced growth in Swiss albino mice. The benefits of both treatments are discussed and interpreted. Methods Fifty adult male mice were randomly divided into two groups; ten mice in the first group served as control group and forty in the second group which received a single dose IP injection of tumor fluid (0.02 Ml) to induce tumor. Ten days post injection to allow the tumor to growth, the 40 mice were sub- divided in to 4 sub-groups 10 mice pre each to introduce the treatment. Results The results indicated tumor growth inhibition regarding mean tumor volume variation (ml) presented. All treatments display tumor growth prevention effect compared to control untreated mice. Treatment with Dox + 7G (MF) exposure exhibited a significant inhibition of tumor growth than that treated alone with DOX or magnetic field; 82% inhibition for DOX + MF 7 G against 60% for 7 G , and 31% for DOX only. Optical density data show a higher values of the molar absorption coefficient for all treated groups than untreated one. The fluorescence emission spectra of Hb show an emission peaks λ em at 465, 515, and 639 nm. Hematological examination might indicate to discriminative effects to RBCs, WBCs or/and Hb for all treated groups. Moreover, treatment with Dox + 7G MF shows a proper discriminative effects than that treatment with DOX or magnetic field only. Osmotic fragility (OF) test indicates that the combination between drug and magnetic field have nontoxic effect against RBCs membrane. Conclusion Our findings support further exploration of the potential of magnetic fields in cancer therapeutics, either as adjunct or primary therapy. It may be due to enhancing the drug interaction with tumor cells which increase the therapeutic index of DOX and resulted in increased anti-tumor activity against Ehrlich tumor models. These benefits promote the use of the magnetic field in cancer with chemotherapy over the other traditional treatment agents this highly adapted manner can be used in improving the clinical treatment protocol and fights against cancer.

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Section: Discussionsupporting

confidence: 92%

Magnetic field potential effects on the doxorubicin therapeutic activity in Ehrlich tumor growth

Ghannam

Al-Otaibi

Alanazy

et al. 2021

Saudi Journal of Biological Sciences

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“…Masilamani et al observed enhanced fluorescence bands (one around 590 nm and another around 630 nm) in blood samples for several different types of cancers, such as gastric cancer, breast cancer, and Hodgkin's lymphoma [40]. Kalaivani et al found elevated porphyrin fluorescence in the blood components of cervical cancer patients [36]. It indicates that porphyrins accumulate more in tumor parts.…”

Section: Discussionmentioning

confidence: 97%

“…The spectra were normalized to 100% at the peak maximum and then calculated with curve-fitting. The wide spectra were contributed by several endogenous fluorophores, such as free NADH, protein-bound NADH, free FAD, basic form, and neutral form of porphyrins [36], tryptophan, collagen, and elastin. The fluorescence of protein-bound FAD is very weak [37] and not considered in this study.…”

Section: Autofluorescence Spectra Of Lung Tissue Samplesmentioning

confidence: 99%

Autofluorescence Imaging and Spectroscopy of Human Lung Cancer

et al. 2016

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Lung cancer is one of the most common cancers, with high mortality rate worldwide. Autofluorescence imaging and spectroscopy is a non-invasive, label-free, real-time technique for cancer detection. In this study, lung tissue sections excised from patients were detected by laser scan confocal microscopy and spectroscopy. The autofluorescence images demonstrated the cellular morphology and tissue structure, as well as the pathology of stained images. Based on the spectra study, it was found that the majority of the patients showed discriminating fluorescence in tumor tissues from normal tissues. Therefore, autofluorescence imaging and spectroscopy may be a potential method for aiding the diagnosis of lung cancer.

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“…There has been some reports using native fluorescence spectroscopy for cervical cancer detection on blood samples [26,27], urine samples [27], animal models [28], cell lines [29,30], human cervical tissue sections [24,31,32] and in vivo by colposcopy [33]. Most of the studies reported the intensity difference between healthy and cancerous samples, but the sensitivities in the literature are variable, ranging from 62% to 92% [26,33].…”

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confidence: 99%

Label‐free imaging and spectroscopy for early detection of cervical cancer

Jing

Wang

et al. 2018

Journal of Biophotonics

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The label-free imaging and spectroscopy method was studied on cervical unstained tissue sections obtained from 36 patients. The native fluorescence spectra of tissues are analyzed by the optical redox ratio (ORR), which is defined as fluorescence intensity ratio between NADH and FAD, and indicates the metabolism change with the cancer development. The ORRs of normal tissues are consistently higher than those of precancer or cancerous tissues. A criterion line of ORR at 5.0 can be used to discriminate cervical precancer/cancer from normal tissues. The sensitivity and specificity of the native fluorescence spectroscopy method for cervical cancer diagnosis are determined as 100% and 91%. Moreover, the native fluorescence spectroscopy study is much more sensitive on the healthy region of cervical precancer/cancer patients compared with the traditional clinical staining method. The results suggest label-free imaging and spectroscopy is a fast, highly sensitive and specific method on the detection of cervical cancer.

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Cervical cancer detection by time-resolved spectra of blood components

Cited by 12 publications

References 17 publications

Magnetic field potential effects on the doxorubicin therapeutic activity in Ehrlich tumor growth

Magnetic field potential effects on the doxorubicin therapeutic activity in Ehrlich tumor growth

Autofluorescence Imaging and Spectroscopy of Human Lung Cancer

Label‐free imaging and spectroscopy for early detection of cervical cancer

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