Fluorescence polarization gene assay for HIV-DNA based on the use of dendrite-modified gold nanoparticles acting as signal amplifiers

Liang, Shuping; He, Guicheng; Tian, Jianniao; Zhao, Yi; Zhao, Shulin

doi:10.1007/s00604-018-2673-1

Cited by 18 publications

(7 citation statements)

References 33 publications

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“…As a result, the rotation speed of the labeled DNA probe slowed down, causing an increase in fluorescence polarization and system sensitivity. Experimental results showed that HIV-DNA could be detected in concentrations as low as 73 pM, and that when HIV-DNA concentrations was within 150 pM to 6 nM, HIV-DNA concentration and FP had a good linear relationship [ 37 ].…”

Section: Nanostructured Fp-based Biosensorsmentioning

confidence: 99%

Nanomaterials Used in Fluorescence Polarization Based Biosensors

Zhang

Tang

Chen

et al. 2022

IJMS

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Fluorescence polarization (FP) has been applied in detecting chemicals and biomolecules for early-stage diagnosis, food safety analyses, and environmental monitoring. Compared to organic dyes, inorganic nanomaterials such as quantum dots have special fluorescence properties that can enhance the photostability of FP-based biosensing. In addition, nanomaterials, such as metallic nanoparticles, can be used as signal amplifiers to increase fluorescence polarization. In this review paper, different types of nanomaterials used in in FP-based biosensors have been reviewed. The role of each type of nanomaterial, acting as a fluorescent element and/or the signal amplifier, has been discussed. In addition, the advantages of FP-based biosensing systems have been discussed and compared with other fluorescence-based techniques. The integration of nanomaterials and FP techniques allows biosensors to quickly detect analytes in a sensitive and cost-effective manner and positively impact a variety of different fields including early-stage diagnoses.

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Section: Nanostructured Fp-based Biosensorsmentioning

confidence: 99%

Nanomaterials Used in Fluorescence Polarization Based Biosensors

Zhang

Tang

Chen

et al. 2022

IJMS

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“…First, 13 nm AuNPs were prepared by sodium citrate reduction according to a previously reported method. − Briefly, 2 mL of 1% HAuCl 4 mixed with 49 mL of H 2 O was heated to boiling with continuous stirring. Subsequently, 570 μL of 10% sodium citrate was quickly added under vigorous stirring, and then the solution was continued to boil for 15 min after the color of the solution changed from light yellow to deep red.…”

Section: Experiments Sectionmentioning

confidence: 99%

DNA Logic Nanodevices for Real-Time Monitoring of ATP in Lysosomes

Wang

et al. 2021

Anal. Chem.

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DNA logic nanodevices have prospects in molecular recognitions but still face challenges in achieving DNA computation-controlled regulation in specific compartments of living cells. By incorporating the i-motif sequence and ATP aptamers into a Y-shaped DNA (Y-DNA) structure, and applying gold nanoparticles (AuNPs) as the transporting carrier, herein we present a new type of DNA logic nanodevices to monitor the ATP levels in lysosomes of living cells. Triple energy transfers including dual fluorescent resonance energy transfers (FRETs) and a nanometal surface energy transfer (NSET) occurred in the DNA logic nanodevices. It was identified that the proposed nanodevices perform an AND logic operation to output FRET signals only when an endogenous proton and ATP simultaneously exist in the cellular microenvironment. Owing to the use of the i-motif sequence, the nanodevices have lysosome-recognizing capacity without causing alkalization of the acidic organelle, making DNA computationcontrolled regulation at the level of cellular organelles achievable. These DNA logic nanodevices show high application prospects in lysosome-related cellular function and disease treatment.

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“…To enhance the diagnosis sensitivity, numerous nanomaterials such as carbon nanotubes [91], AuNPs [92], and Ag nanoparticle [93], etc were utilized to amplify fluorescence polarization signals. Liang and coworkers constructed a fluorescence polarization assay system for diagnosis of HIV-DNA through AuNPs self-assembled by the functional dendritic macromolecules [94]. Firstly, AuNPs loaded by two DNA probes as reporters were prepared.…”

Section: Fluorescence Polarization Assaymentioning

confidence: 99%

Nanotechnology based strategies for HIV-1 and HTLV-1 retroviruses gene detection

Mozhgani

Kermani

Norouzi

et al. 2020

Heliyon

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Early detection of retroviruses including human T-cell lymphotropic virus and human immunodeficiency virus in the human body is indispensable to prevent retroviral infection propagation and improve clinical treatment. Until now, diverse techniques have been employed for the early detection of viruses. Traditional methods are timeconsuming, resource-intensive, and laborious performing. Therefore, designing and constructing a selective and sensitive diagnosis system to detect serious diseases is highly demanded. Genetic detection with high sensitivity has striking significance for the early detection and remedy of disparate pathogenic diseases. The nucleic acid biosensors are based on the identification of specific DNA sequences in biological samples. Nanotechnology has an important impact on the development of sensitive biosensors. Different kinds of nanomaterials include nanoparticles, nanoclusters, quantum dots, carbon nanotubes, nanocomposites, etc., with different properties have been used to improve the performance of biosensors. Recently, DNA nanobiosensors are developed to provide simple, fast, selective, low-cost, and sensitive detection of infectious diseases. In this paper, the research progresses of nano genosensors for the detection of HIV-1 and HTLV-1 viruses, based on electrochemical, optical, and photoelectrochemical platforms are overviewed.

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Fluorescence polarization gene assay for HIV-DNA based on the use of dendrite-modified gold nanoparticles acting as signal amplifiers

Cited by 18 publications

References 33 publications

Nanomaterials Used in Fluorescence Polarization Based Biosensors

Nanomaterials Used in Fluorescence Polarization Based Biosensors

DNA Logic Nanodevices for Real-Time Monitoring of ATP in Lysosomes

Nanotechnology based strategies for HIV-1 and HTLV-1 retroviruses gene detection

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