DNA Logic Nanodevices for Real-Time Monitoring of ATP in Lysosomes

Li, Li Li; Lv, Wen; Wang, Yao; Li, Yuan Fang; Li, Chun Mei; Huang, Cheng

doi:10.1021/acs.analchem.1c02829

Cited by 12 publications

(10 citation statements)

References 45 publications

(56 reference statements)

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“…DNA biocomputing nanodevices (DBNs), owing to high programmability, multiple logic inputs, and good biocompatibility, have been widely applied in molecular imaging. − However, they suffer from seldom focusing on molecular events and poor spatial selectivity in tumor cells. − Furthermore, DNA logic nanodevices for spatially selective imaging of tumor cells have been initially tried and successfully constructed, but there are problems such as low detection sensitivity and slow operation.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Response of Mitochondrial MicroRNAs to Identify Cell Apoptosis with Multiple Responsive Intelligent DNA Biocomputing Nanodevices

Cheng

et al. 2023

Anal. Chem.

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Challenges remained in precisely real-time monitoring of apoptotic molecular events at the subcellular level. Herein, we developed a new type of intelligent DNA biocomputing nanodevices (iDBNs) that responded to mitochondrial microRNA-21 (miR-21) and microRNA-10b (miR-10b) simultaneously which were produced during cell apoptosis. By hybridizing two hairpins (H1 and H2) onto DNA nanospheres (DNSs) that had been previously modified with mitochondria-targeted triphenylphosphine (TPP) motifs, iDBNs were assembled in which two localized catalytic hairpins self-assembly (CHA) reactions occurred upon the co-stimulation of mitochondrial miR-21 and miR-10b to perform AND logic operations, outputting fluorescence resonance energy transfer (FRET) signals for sensitive intracellular imaging during cell apoptosis. Owing to the spatial confinement effects of DNSs, it was discovered that iDBNs had a high efficiency and speed of logic operations by high local concentrations of H1 and H2, making the simultaneous real-time responses of mitochondrial miR-21 and miR-10b reliable and sensitive during cell apoptosis. These results demonstrated that iDBNs were simultaneously responsive to multiple biomarkers, which greatly improved the detection accuracy to identify the cell apoptosis, demonstrating that iDBNs are highly effective and reliable for the diagnosis of major disease and screening of anticancer drugs.

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Section: Introductionmentioning

confidence: 99%

Simultaneous Response of Mitochondrial MicroRNAs to Identify Cell Apoptosis with Multiple Responsive Intelligent DNA Biocomputing Nanodevices

Cheng

et al. 2023

Anal. Chem.

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“…A similar Y-motif DNA circuit incorporating with mere ATP aptamer has also been applied for real-time monitoring of ATP in lysosomes. [131] Very recently, Chao et al designed a pH-responsive DNAzyme walker that enabled multilayer DNA cascades for precise sensing of intracellular biomolecules (Figure 5C). [132] The four-stranded DNAzyme walker precursor was anchored on the surface of AuNP decorated with substrate strands.…”

Section: Ph-responsive Dna Circuit For Bioimagingmentioning

confidence: 99%

Stimuli‐Responsive DNA Circuits for High‐Performance Bioimaging Application

Shang

et al. 2023

Advanced Sensor Research

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Probing endogenous molecules in living entities is significant to help decipher biological functions and exploit novel theranostics. DNA circuits that can recognize molecular inputs of interest and transduce them into readable signal outputs in an isothermal and autonomous manner have been actively pursued as versatile toolkits for intracellular biosensing research. Tremendous efforts are being devoted to developing integrated DNA circuits with high sensitivity, while spatiotemporal selectivity is often overlooked in the construction of functional DNA circuitry systems. This requires the development of stimuli‐responsive DNA circuits that can be activated on‐demand from the initial sensing‐blunt state to the sensing‐ready state under a programmable manner, for achieving precise bioimaging with high spatiotemporal control. In this review, an overview of recent advances in the construction of stimuli‐responsive DNA circuits that respond to particular triggers, including external physical stimuli and endogenous biological cues, and their spatiotemporally controllable molecular bioimaging applications is provided. The current challenges and potential solutions of these stimuli‐responsive DNA circuits for their future developments in this emerging field are also discussed.

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“…For example, the fast cutting of nuclease and DNAzyme can greatly improve the walking speed of the DNA walker, but enzyme-driven DNA walkers often require the addition of enzymes or auxiliary metal ions from the outside to provide walking power in intracellular imaging, which complicates the cell treatment and makes it difficult to control the distribution of each component of the DNA walkers in live cells, increasing the signal error rate. To address this problem, endogenous adenosine triphosphate (ATP)-driven DNA walkers have been developed recently. , As the energy source of vital cellular life activities, ATP abound in the tumor microenvironment ( TME ) − which can be an efficient cofactor to power the DNA walkers.…”

Section: Introductionmentioning

confidence: 99%

Endogenous Adenosine Triphosphate-Assisted Three-Dimensional DNA Walker Assembled on Soft Nanoparticles for Intracellular MicroRNA Imaging

et al. 2023

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DNA walkers, a sophisticated type of nanomachines, exhibit intelligent application in biosensing with high programmability and flexibility but usually need additional auxiliary driving force, particularly when walking on hard surfaces. Herein, we construct a three-dimensional (3D) DNA walker on the soft surface of DNA nanospheres (DSs) by using a single-stranded DNA (ssDNA), which is powered by endogenous adenosine triphosphate (ATP) of live cells, so as to sensitively image microRNA (miRNA) in the tumor microenvironment. When the DS walker enters into live cells, miR-21, a general overexpressed biomarker in cancer cells, binds with the blocking strand (B), releasing the walking strand (W) and triggering an ATPpropelled walking reaction. The walking of the DS walker then generates an increasing Cy3 fluorescence signal that indicates the content of miR-21 with about 2.73-fold increase in sensitivity and about 157-fold decrease in the detection limit. Notably, the assembly of the DS walker on soft nanoparticles needs just an easy hybridization process, which facilitates the operation. Meanwhile, this endogenous ATP-powered 3D DNA walker walking on the soft surface performs real-time in situ imaging of miR-21 in live cells, which not only avoids the complex cell treatment and signal error induced by additional auxiliary factors, but also shows high promise of designing programmable DNA nanomachines.

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DNA Logic Nanodevices for Real-Time Monitoring of ATP in Lysosomes

Cited by 12 publications

References 45 publications

Simultaneous Response of Mitochondrial MicroRNAs to Identify Cell Apoptosis with Multiple Responsive Intelligent DNA Biocomputing Nanodevices

Simultaneous Response of Mitochondrial MicroRNAs to Identify Cell Apoptosis with Multiple Responsive Intelligent DNA Biocomputing Nanodevices

Stimuli‐Responsive DNA Circuits for High‐Performance Bioimaging Application

Endogenous Adenosine Triphosphate-Assisted Three-Dimensional DNA Walker Assembled on Soft Nanoparticles for Intracellular MicroRNA Imaging

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