Fluorescence and photochemical properties of phytochromes A and B in etiolated pea seedlings

Sineshchekov, V.A.; Ogorodnikova, Ouliana B.; Weller, James L.

doi:10.1016/s1011-1344(99)00061-5

Cited by 23 publications

(33 citation statements)

References 27 publications

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“…Growth and harvest of plant material and spectrophotometric measurements of phytochrome were carried out according to previously described methods (Weller et al, 1995;Sineshchekov et al, 1999).…”

Section: Spectrophotometric Measurementsmentioning

confidence: 99%

A Dominant Mutation in the Pea PHYA Gene Confers Enhanced Responses to Light and Impairs the Light-Dependent Degradation of Phytochrome A

Weller

Batge²,

Smith³

et al. 2004

Plant Physiology

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Phytochrome A (phyA) is an important photoreceptor controlling many processes throughout the plant life cycle. It is unique within the phytochrome family for its ability to mediate photomorphogenic responses to continuous far-red light and for the strong photocontrol of its transcript level and protein stability. Here we describe a dominant mutant of garden pea (Pisum sativum) that displays dramatically enhanced responses to light, early photoperiod-independent flowering, and impaired photodestruction of phyA. The mutant carries a single base substitution in the PHYA gene that is genetically inseparable from the mutant phenotype. This substitution is predicted to direct the replacement of a conserved Ala in an N-terminal region of PHYA that is highly divergent between phyA and other phytochromes. This result identifies a region of the phyA photoreceptor molecule that may play an important role in its fate after photoconversion.

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Section: Spectrophotometric Measurementsmentioning

confidence: 99%

A Dominant Mutation in the Pea PHYA Gene Confers Enhanced Responses to Light and Impairs the Light-Dependent Degradation of Phytochrome A

Weller

Batge²,

Smith³

et al. 2004

Plant Physiology

View full text Add to dashboard Cite

show abstract

“…The shape of the spectrum of the old light-treated roots was very close to that of the phyAphyB-deficient pea mutant [13]. (c, f) -seedlings grown under far-red light (k a P 720 nm).…”

Section: Equipment and Measurement Proceduresmentioning

confidence: 69%

“…3: Spectrum of 11-day-old wild-type pea root sections at its base after saturating red (633 nm) illumination to convert Pr into the far-red absorbing form (Pfr). Spectrum 3 is very close to the spectrum of etiolated seedlings of the double mutant [13] lacking phyA and phyB (phyAphyB) suggesting that the sample 3 contains almost no phytochrome. The spectrum is taken as a spectrum of background emission for corrections of the experimental spectra 1 and 2 to get real spectra of phytochrome (see Fig.…”

Section: Equipment and Measurement Proceduresmentioning

confidence: 80%

“…The experimental approach was the same as that used earlier (see [11][12][13][14]17,18,21,22] and the literature cited therein). To obtain the phytochrome parameters in the sample, low-temperature (85 K) emission spectra (wavelength of the excitation, k e , 610 or 650 nm) were taken under two different experimental settings (see Figs.…”

Section: Equipment and Measurement Proceduresmentioning

confidence: 99%

“…With the use of in situ low-temperature fluorescence spectroscopy, two phyA native species, phyA 0 and phyA 00 , were found in experiments on phyBdeficient mutants (of cucumber [11], Arabidopsis [12] and pea [13]) and on roots of the wild type tobacco, which according to [6] contains almost no phyB (V. Sineshchekov, unpublished results). The most pronounced phenomenological difference of the two phyA species, which allows their quantitative assay in plant tissues, is high and low extent (c 1 ) of the Pr conversion into the first photoproduct, lumi-R, %0.5 for phyA 0 and %0.05 for phyA 00 (Pr 0 and Pr 00 phenomenological types, respectively).…”

Section: Introductionmentioning

confidence: 99%

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