A Dominant Mutation in the Pea <i>PHYA</i> Gene Confers Enhanced Responses to Light and Impairs the Light-Dependent Degradation of Phytochrome A

Weller, James L.; Batge, Shona L.; Smith, Jennifer J.; Kerckhoffs, L. H. J.; Sineshchekov, V.A.; Murfet, Ian C.; Reid, James B.

doi:10.1104/pp.103.036103

Cited by 47 publications

(32 citation statements)

References 67 publications

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“…More recent studies have identified genes necessary for promotion of flowering under inductive LD photoperiods, including PHYA (Weller et al, 2004) and LATE1, the ortholog of Arabidopsis GI (Hecht et al, 2007), but the primary physiological role and molecular nature of the SN, DNE, and PPD loci have remained unclear. Here, we show that DNE is necessary for the normal rhythmic regulation of circadian clock genes and identify DNE as the pea ortholog of Arabidopsis ELF4.…”

Section: Discussionmentioning

confidence: 99%

DIE NEUTRALIS and LATE BLOOMER 1 Contribute to Regulation of the Pea Circadian Clock

et al. 2009

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The DIE NEUTRALIS (DNE) locus in garden pea (Pisum sativum) was previously shown to inhibit flowering under noninductive short-day conditions and to affect a graft-transmissible flowering signal. In this study, we establish that DNE has a role in diurnal and/or circadian regulation of several clock genes, including the pea GIGANTEA (GI) ortholog LATE BLOOMER 1 (LATE1) and orthologs of the Arabidopsis thaliana genes LATE ELONGATED HYPOCOTYL and TIMING OF CHLOROPHYLL A/B BINDING PROTEIN EXPRESSION 1. We also confirm that LATE1 participates in the clock and provide evidence that DNE is the ortholog of Arabidopsis EARLY FLOWERING4 (ELF4). Circadian rhythms of clock gene expression in wild-type plants under constant light were weaker in pea than in Arabidopsis, and a number of differences were also seen in the effects of both DNE/ELF4 and LATE1/GI on clock gene expression. Grafting studies suggest that DNE controls flowering at least in part through a LATE1-dependent mobile stimulus, and dne mutants show elevated expression of a FLOWERING LOCUS T homolog under short-day conditions. However, the early flowering of the dne mutant is not associated with altered expression of a previously described CONSTANS-like gene. Collectively, our results characterize the clock system and reveal its importance for photoperiod responsiveness in a model legume.

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Section: Discussionmentioning

confidence: 99%

DIE NEUTRALIS and LATE BLOOMER 1 Contribute to Regulation of the Pea Circadian Clock

et al. 2009

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“…each photoreceptor, although few data are available to test the conservation of gene function in seed plants other than Arabidopsis. Nonetheless, the available data from Brassica, cucumber (Cucumis sativus), pea (Pisum sativum), tobacco (Nicotiana tabacum), tomato (Solanum lycopersicum), maize (Zea mays), and rice (Oryza sativa) suggest that the functions of phyA and phyB are generally conserved and were established prior to the split of eudicots and monocots (Ballaré et al, 1991;Childs et al, 1991;Devlin et al, 1992;Weller et al, 2004;Sheehan et al, 2007;Takano et al, 2005Takano et al, , 2009, although independently duplicated phyB may subdivide functions differently than is seen in Arabidopsis phyB and phyD (e.g., Hudson et al, 1997;Kerckhoffs et al, 1999;Weller et al, 2000;Sheehan et al, 2007). Functional data from dicots that diverge deeper than the eudicot/monocot split in the angiosperm phylogenetic tree are needed to infer that their functions are conserved in all angiosperms, but this is a reasonable hypothesis.…”

Section: Conservation Of Phya and Phyb Functionmentioning

confidence: 99%

Evolutionary Studies Illuminate the Structural-Functional Model of Plant Phytochromes

Mathews

2010

The Plant Cell

107

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A synthesis of insights from functional and evolutionary studies reveals how the phytochrome photoreceptor system has evolved to impart both stability and flexibility. Phytochromes in seed plants diverged into three major forms, phyA, phyB, and phyC, very early in the history of seed plants. Two additional forms, phyE and phyD, are restricted to flowering plants and Brassicaceae, respectively. While phyC, D, and E are absent from at least some taxa, phyA and phyB are present in all sampled seed plants and are the principal mediators of red/far-red-induced responses. Conversely, phyC-E apparently function in concert with phyB and, where present, expand the repertoire of phyB activities. Despite major advances, aspects of the structural-functional models for these photoreceptors remain elusive. Comparative sequence analyses expand the array of locus-specific mutant alleles for analysis by revealing historic mutations that occurred during gene lineage splitting and divergence. With insights from crystallographic data, a subset of these mutants can be chosen for functional studies to test their importance and determine the molecular mechanism by which they might impact light perception and signaling. In

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“…Although the majority of phytochrome mutants so far identified have been loss-of-function alleles (for a recent list of phy mutant alleles, see Supplemental Table 2 in ), alleles of phytochromes with increased sensitivity to light and weak constitutive photomorphogenesis (COP) phenotypes have also been reported (Kretsch et al, 2000;Weller et al, 2004;Dieterle et al, 2005;Mateos et al, 2006). The latter are still dependent on light for function, so their functions cannot be analyzed without activating other photobiological processes.…”

Section: Introductionmentioning

confidence: 99%

Light-Independent Phytochrome Signaling Mediated by Dominant GAF Domain Tyrosine Mutants ofArabidopsisPhytochromes in Transgenic Plants

2007

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The photoreversibility of plant phytochromes enables continuous surveillance of the ambient light environment. Through expression of profluorescent, photoinsensitive Tyr-to-His mutant alleles of Arabidopsis thaliana phytochrome B (PHYBY276H) and Arabidopsis phytochrome A (PHYAY242H) in transgenic Arabidopsis plants, we demonstrate that photoconversion is not a prerequisite for phytochrome signaling. PHYBY276H-expressing plants exhibit chromophore-dependent constitutive photomorphogenesis, light-independent phyBY276H nuclear localization, constitutive activation of genes normally repressed in darkness, and light-insensitive seed germination. Fluence rate analyses of transgenic plants expressing PHYBY276H, PHYAY242H, and other YGAF mutant alleles of PHYB demonstrate that a range of altered light-signaling activities are associated with mutation of this residue. We conclude that the universally conserved GAF domain Tyr residue, with which the bilin chromophore is intimately associated, performs a critical role in coupling light perception to signal transduction by plant phytochromes.

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A Dominant Mutation in the Pea PHYA Gene Confers Enhanced Responses to Light and Impairs the Light-Dependent Degradation of Phytochrome A

Cited by 47 publications

References 67 publications

DIE NEUTRALIS and LATE BLOOMER 1 Contribute to Regulation of the Pea Circadian Clock

DIE NEUTRALIS and LATE BLOOMER 1 Contribute to Regulation of the Pea Circadian Clock

Evolutionary Studies Illuminate the Structural-Functional Model of Plant Phytochromes

Light-Independent Phytochrome Signaling Mediated by Dominant GAF Domain Tyrosine Mutants ofArabidopsisPhytochromes in Transgenic Plants

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